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Method for producing conjugated linolic acid by using linolic acid isomerase

A technology of linoleic acid isomerase and conjugated linoleic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, bulk chemical production, etc., can solve the problem of reducing product quality, limiting reaction rate, and high product cost and other issues to achieve the effect of reducing production costs and improving conversion rates

Active Publication Date: 2011-04-13
中农科合肥食品营养与健康创新研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, organic solvents have the following problems: first, the large multiphase mass transfer resistance limits the improvement of the reaction rate; second, the solvent residue reduces the product quality, which is not tolerated by the "green food" market; in addition, the higher organic solvent The price greatly increases the cost of the product

Method used

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  • Method for producing conjugated linolic acid by using linolic acid isomerase
  • Method for producing conjugated linolic acid by using linolic acid isomerase
  • Method for producing conjugated linolic acid by using linolic acid isomerase

Examples

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Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, utilize linoleic acid isomerase to produce conjugated linoleic acid

[0047] 1. Extraction and purification of linoleic acid isomerase

[0048] 1. Obtainment of linoleic acid isomerase fermentation broth

[0049] The fermentation medium used in this embodiment is prepared according to the following method:

[0050] (1) Preparation of sunflower oil emulsion: sunflower oil (Shanghai Jiage Food Co., Ltd., Duoli brand 100% pure sunflower oil) 300mg, Tween-80 (Tween-80) 0.36ml, deionized water 5ml , ultrasonic emulsification (25 ℃, ultrasonic frequency 40KHz) 10min, 0.22μm microporous filter membrane filter sterilization to obtain sunflower oil emulsion.

[0051] (2) Preparation of skim milk medium: 12 g of skim milk powder (Sanyuan Dairy Co., Ltd.) was dissolved in 100 ml of water, and sterilized at 115° C. for 10 min for later use.

[0052] (3) Preparation of fermentation medium: the sunflower oil emulsion of (1) was added to the skim milk medium of (2), s...

Embodiment 2

[0070] Embodiment 2, utilize linoleic acid isomerase to produce conjugated linoleic acid

[0071] 1. Obtainment of linoleic acid isomerase fermentation broth

[0072] The fermentation medium used in this embodiment is prepared according to the following method:

[0073] The fermentation medium used in this embodiment is prepared according to the following method:

[0074] (1) Preparation of sunflower oil emulsion: sunflower oil (Shanghai Jiage Food Co., Ltd., Duoli brand 100% pure sunflower oil) 400mg, Tween-80 (Tweenum 80) 0.44ml, deionized water 5ml, Ultrasonic emulsification (25° C., ultrasonic frequency 40KHz) for 10min, 0.22 μm microporous filter membrane filter sterilization, to obtain sunflower oil emulsion.

[0075] (2) Preparation of skim milk medium: 12 g of skim milk powder (provided by Beijing Sanyuan Dairy Co., Ltd.) was dissolved in 100 ml of water, and sterilized at 115° C. for 10 min for later use.

[0076] (3) Preparation of fermentation medium: the sunflow...

Embodiment 3

[0094] Embodiment 3, utilize linoleic acid isomerase to produce conjugated linoleic acid

[0095] 1. Obtainment of linoleic acid isomerase fermentation broth

[0096] The fermentation medium used in this embodiment is prepared according to the following method:

[0097] (1) Preparation of sunflower oil emulsion: sunflower oil (Shanghai Jiage Food Co., Ltd., Duoli brand 100% pure sunflower oil) 600mg, Tween-80 (Tween-80) 0.5ml, deionized water 5ml , ultrasonic emulsification (25 ℃, ultrasonic frequency 40KHz) 10min, 0.22μm microporous filter membrane filter sterilization to obtain sunflower oil emulsion.

[0098] (2) Preparation of skim milk medium: 12 g of skim milk powder (Sanyuan Dairy Co., Ltd.) was dissolved in 100 ml of water, and sterilized at 115° C. for 10 min for later use.

[0099] (3) Preparation of fermentation medium: the sunflower oil emulsion of (1) was added to the skim milk medium of (2), so that the final concentration of sunflower oil was 6 mg / ml to obtain...

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Abstract

The invention discloses a method to produce conjugate linolic acid with linolic acid isomerase, which comprises the following steps: reacting linolic acid and linolic acid isomerase in hypercritical carbon dioxide for 1-3h; setting pressure of the reaction at 25-35 Mpa and temperature at 32-44 deg. c; getting conjugate linolic acid. This invention use hypercritical fluid, hypercritical carbon dioxide as media in enzyme catalytic reaction, thereby decreasing the cost and increasing the conversion rate of conjugate linolic acid.

Description

technical field [0001] The present invention relates to a method for producing conjugated linoleic acid by utilizing linoleic acid isomerase. Background technique [0002] Conjugated Linoleic Acid (CLA) is a very interesting nutritional additive. A large number of in vitro and in vivo experiments have shown that CLA can induce energy utilization and lead to weight loss without storing fat; it can alleviate symptoms such as anorexia. , protein decomposition and other side effects of immune reactions; it has anti-mutation, inhibits the synthesis of proteins and nucleic acids in cancer cells, and CLA can also improve certain physiological states of humans, such as increasing the level of vitamin A in the body. Because CLA has rich nutritional value and the content in food is very low, how to prepare CLA with low cost and high purity has become the focus of research. The commercial production method of CLA is the alkali isomerization method of linoleic acid. The raw material of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/225C12R1/645C12R1/25C12R1/02C12P7/64C12R1/63
CPCY02P20/54
Inventor 吕加平王丽敏任星环江均平
Owner 中农科合肥食品营养与健康创新研究院有限公司
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