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Method for producing conjugated linolic acid by using linolic acid isomerase

A technology of linoleic acid isomerase and conjugated linoleic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, bulk chemical production, etc., can solve the problem of reducing product quality, limiting reaction rate, and high product cost and other issues to achieve the effect of reducing production costs and improving conversion rates

Active Publication Date: 2007-10-31
中农科合肥食品营养与健康创新研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, organic solvents have the following problems: first, the large multiphase mass transfer resistance limits the improvement of the reaction rate; second, the solvent residue reduces the product quality, which is not tolerated by the "green food" market; in addition, the higher organic solvent The price greatly increases the cost of the product

Method used

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  • Method for producing conjugated linolic acid by using linolic acid isomerase
  • Method for producing conjugated linolic acid by using linolic acid isomerase
  • Method for producing conjugated linolic acid by using linolic acid isomerase

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1, utilize linoleic acid isomerase to produce conjugated linoleic acid

[0047] 1. Extraction and purification of linoleic acid isomerase

[0048] 1. Obtaining of linoleic acid isomerase fermentation broth

[0049] The fermentation medium used in this embodiment is prepared according to the following method:

[0050] (1) Preparation of sunflower oil emulsion: 300 mg of sunflower oil (Shanghai Jiage Food Co., Ltd., Duoli brand 100% pure sunflower oil), 0.36 ml of Tween-80 (Tween-80), 5 ml of deionized water , ultrasonic emulsification (25° C., ultrasonic frequency 40 KHz) for 10 min, and filter sterilization with a 0.22 μm microporous membrane to obtain a sunflower oil emulsion.

[0051] (2) Preparation of skim milk medium: 12 g of skim milk powder (Sanyuan Dairy Co., Ltd.) was dissolved in 100 ml of water, and sterilized at 115° C. for 10 minutes for later use.

[0052] (3) Preparation of fermentation medium: the sunflower oil emulsion of (1) was added to th...

Embodiment 2

[0070] Embodiment 2, utilize linoleic acid isomerase to produce conjugated linoleic acid

[0071] 1. Obtaining of linoleic acid isomerase fermentation broth

[0072] The fermentation medium used in this embodiment is prepared according to the following method:

[0073] The fermentation medium used in this embodiment is prepared according to the following method:

[0074] (1) Preparation of sunflower seed oil emulsion: 400 mg of sunflower oil (Shanghai Jiage Food Co., Ltd., Duoli brand 100% pure sunflower oil), Tween-80 (Tweenum 80) 0.44 ml, deionized water 5 ml, Ultrasonic emulsification (25° C., ultrasonic frequency 40 KHz) for 10 minutes, and filter sterilization with a 0.22 μm microporous membrane to obtain sunflower oil emulsion.

[0075] (2) Preparation of skim milk medium: 12 g of skim milk powder (provided by Beijing Sanyuan Dairy Co., Ltd.) was dissolved in 100 ml of water, and sterilized at 115° C. for 10 min for later use.

[0076] (3) Preparation of fermentation ...

Embodiment 3

[0094] Embodiment 3, utilize linoleic acid isomerase to produce conjugated linoleic acid

[0095] 1. Obtaining of linoleic acid isomerase fermentation broth

[0096] The fermentation medium used in this embodiment is prepared according to the following method:

[0097] (1) Preparation of sunflower seed oil emulsion: 600mg of sunflower seed oil (Shanghai Jiage Food Co., Ltd., Duoli brand 100% pure sunflower seed oil), 0.5ml of Tween-80 (Tween-80), 5ml of deionized water , ultrasonic emulsification (25° C., ultrasonic frequency 40 KHz) for 10 min, and filter sterilization with a 0.22 μm microporous membrane to obtain a sunflower oil emulsion.

[0098] (2) Preparation of skim milk medium: 12 g of skim milk powder (Sanyuan Dairy Co., Ltd.) was dissolved in 100 ml of water, and sterilized at 115° C. for 10 minutes for later use.

[0099] (3) Preparation of fermentation medium: the sunflower oil emulsion of (1) was added to the skim milk medium of (2), so that the final concentrat...

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Abstract

The invention discloses a method to produce conjugate linolic acid with linolic acid isomerase, which comprises the following steps: reacting linolic acid and linolic acid isomerase in hypercritical carbon dioxide for 1-3h; setting pressure of the reaction at 25-35MPa and temperature at 32-44 deg. c; getting conjugate linolic acid. This invention can decrease the cost and increase the conversion rate of conjugate linolic acid.

Description

technical field [0001] The invention relates to a method for producing conjugated linoleic acid by using linoleic acid isomerase. Background technique [0002] Conjugated linoleic acid (Conjugated Linoleic Acid, CLA) is a very interesting nutritional supplement. A large number of in vitro and in vivo experiments have proved that CLA can induce energy utilization and cause weight loss without storing fat; it can alleviate such as anorexia , proteolysis, and other side effects of immune reactions; it has anti-mutation, inhibits the synthesis of proteins and nucleic acids in cancer cells, and in addition, CLA can also improve certain physiological states of humans, such as increasing the level of vitamin A in the body. Since CLA has rich nutritional value and its content in food is very low, how to prepare CLA with low cost and high purity has become the focus of research. The commercial production method of CLA is the alkali isomerization method of linoleic acid. The raw mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12R1/02C12R1/225C12R1/63C12R1/645
CPCY02P20/54
Inventor 吕加平王丽敏任星环江均平
Owner 中农科合肥食品营养与健康创新研究院有限公司
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