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Preparation method for black fungus blood fat-reducing products

A black fungus, lipid-lowering technology, applied in food preparation, food forming, fungi and other directions, can solve the problems of reducing the viscosity of the extract, cumbersome processing, etc., and achieve the effect of reducing serum total cholesterol

Active Publication Date: 2007-10-24
GUANGDONG YUEWEI EDIBLE FUNGI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic extraction process has a certain effect of reducing the viscosity of the extract and improving the extraction yield, but the process of this method is cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Preparation method 1 of black fungus lipid-lowering product

[0024] (1) Preparation of black fungus liquid strain.

[0025] ① Strain activation: Cut out broad bean-sized mycelium inoculum from the test tube of the mother seed, place it in the middle of the PDA slope, and culture it at 26°C for 7 days.

[0026] ②Primary seed cultivation: Based on 1000mL of culture medium, take 1000mL of wort juice (Bx3), 2g of peptone, 2g of KH2PO4, 1g of MgSO4, the pH is natural, and use a 500mL Erlenmeyer flask with a volume of 100mL. The culture solution was sterilized for 30 minutes under the pressure of 0.11MPa, and then the activated slant mother medium was cut into a size of 0.5×0.5cm in the aseptic inoculation room, inserted into 7-8 pieces / bottle, and finally in 28 Cultivate on a shaker at ℃ for 5 days, with a rotation speed of 80 r / min for the first 18 hours, and then change the rotation speed to 100 r / min after 18 hours.

[0027] ③Secondary seed culture: based...

Embodiment 2

[0032] Embodiment 2: Preparation method 2 of black fungus lipid-lowering product

[0033] (1) Preparation of black fungus liquid strain. Prepared according to the method described in Example 1.

[0034](2) Fermentation of black fungus mycelium. Based on 27L culture medium, take 1350g glucose, 2160g bran juice (add 10 times water to boil and filter to remove slag), 135g peptone, 54g KH2PO4, 27g MgSO4, 13.5g bubble enemy by weight percentage, pH is natural, and ferment in 40L liquid Make up the water in the tank to 27L, and then sterilize under the pressure condition of 0.11MPa for 30 minutes. When the culture liquid is cooled to about 30°C, add 1350mL of the above-mentioned secondary fermentation liquid, incubate at 25-30°C, control ventilation at 1:0.5, and cultivate at 150r / min for 3 days.

[0035] (3) The process of extracting and refining black fungus mycelium. After the fermentation is finished, the fermented mycelium and the fermented liquid are passed through the col...

Embodiment 3

[0038] Embodiment 3: Preparation method 3 of black fungus lipid-lowering product

[0039] (1) Preparation of black fungus liquid strain. Prepared according to the method described in Example 1.

[0040] (2) Fermentation of black fungus mycelium. Based on 27L culture medium, take 1350g corn flour, 2160g bran boiled juice (add 10 times water to boil and filter to remove slag), 135g beef extract, 54g KH2PO4, 27g MgSO4, 13.5g foam enemy, pH natural, in 40L Make up water to 27L in the liquid fermenter, then sterilize under 0.11MPa pressure condition for 30 minutes. When the culture liquid is cooled to about 30°C, add 1350mL of the above-mentioned secondary fermentation liquid, at 25-30°C, control the ventilation rate at 1:0.5, and cultivate at 120r / min for 4 days.

[0041] (3) The process of extracting and refining black fungus mycelium. After the fermentation is finished, the fermented mycelium and the fermented liquid are passed through the colloid mill repeatedly for 20 minu...

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PUM

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Abstract

The invention relates to a method for preparing black edible fungus anti-hyperglycemic product, belonging to field of preparing polysaccharide. The invention employs black edible fungus deep level liquid fermentation technique, extracts inter-cellular and extra-cellular polysaccharide from deep level fermentation culture, and getting final product through preparation with cell wall-broken treated black edible fungus powder. The product is characterized by apparent functions of reducing serum cholesterol, triglyceride and low-density lipoprotein, and can be used for subsidiary treatment or prevention for hyperlipoidemia.

Description

【Technical field】 [0001] The invention relates to a preparation method of a black fungus lipid-lowering product, and belongs to the field of preparation methods of polysaccharides. 【Background technique】 [0002] Black fungus (Auricularia auricula (Hook) Underw) is a precious colloidal fungus for both medicine and food in my country. Li Shizhen of the Ming Dynasty recorded in "Compendium of Materia Medica", "Indications, nourishing qi not hungry, making light of body and strengthening will, breaking grains to treat hemorrhoids". Fungus polysaccharide is the main active ingredient of black fungus, which has the functions of lowering blood cholesterol, anticoagulation, and anti-atherosclerosis. However, because black fungus polysaccharides mainly exist on the cell wall, and the cell wall is composed of fungal chitin components, which affects the absorption of active ingredients such as black fungus polysaccharides, so effective ingredients such as black fungus polysaccharides...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/29A23L1/307C12N1/14A23P1/06A23L31/00A23P10/40
Inventor 谢意珍李崇周静文张智李森柱
Owner GUANGDONG YUEWEI EDIBLE FUNGI TECH
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