Method of fixing glucose oxidase by organic modification sol-gel

Abstract

The present invention provides a method for fixing glucose oxidase using organic modified sol- gelatin. Mixing evenly of oxygen radix silane and dimethyl dimethoxy silane, adding HCl aqueous solution of same volume with tetramethyl silane, placing mixing liquor in bottle, covering with lid mounted with a hole, stirring in water bath, placing for 3-5min, reserving gel liquor, mixing prepared gel and polyvinyl alcohol water solution, placing for 3-5min, taking upper clear solution after lamination and mixing with phosphate buffer dissolved with glucose oxidase, applying it on surface of matrix, drying for molding. The invention successfully solves the problem of swelling property derived from using individual PVA embedment. The glucose oxidase is embedded in porous matrix hybridized by gel solution and PVA so it is not easy to be lost and can be stored in a long-term and keeps the activity and stability.

Description

technical field [0001] The invention belongs to a method for preparing an enzyme sensor by embedding enzymes in organically modified sol-gel, and more particularly relates to a method for immobilizing glucose oxidase in organically modified sol-gel. Background technique [0002] Enzyme-catalyzed reactions are characterized by high efficiency, rapidity, and high selectivity, so they have received extensive attention. However, unimmobilized enzymes are usually unstable and difficult to reuse, so immobilized enzyme technology is very necessary. At present, the common enzyme immobilization technologies at home and abroad mainly include: physical adsorption method (Liu et al., Biosensors & Bioelectronics 14 (2000) 883~893; Niculescu et al., Biosensors & Bioelectronics 19 (2004) 1175~1184), total Valence bonding method (Doretti et al., Biosensors & Bioelectronics 11 (1996) 365-373; Li et al., Biomaterials 19 (1998) 45-53), embedding method (Peter et al., Biosensors & Bioelectroni...

AI Technical Summary

Problems solved by technology

Although the immobilization of enzymes by physical adsorption is simple in operation, the stability is poor, the enzymes are easy to lose, and the service life is short; Strong chemical bonding reduces or even inactivates the immobilized enzyme; due to the above shortcomings, the embedding method has become the most widely used immobilization technology so far. Not easy to lose, the pore size and geometry of the membrane can be controlled, high stability, long-term storage

[0003] The sol-gel embedding method can maintain the liquid phase properties of immobilized reagents or microorganisms, which is very suitable for the immobilization of biomolecules or biologically active substances such as bacteria, but the sol-gel method also has problems such as leakage and poor stability.