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Channel augmentative method for fiber sphere separating medium

A technology for separating matrix and cellulose, applied in chemical instruments and methods, other chemical processes, etc., can solve the problem of not developing a macroporous cellulose spherical separation matrix, and achieve easy control and amplification, less environmental pollution, and increased pore size. big effect

Inactive Publication Date: 2007-10-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Both cellulose and starch are very readily available industrial raw materials. So far, there has been no report on the combination of the two to develop a macroporous cellulose spherical separation matrix.

Method used

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  • Channel augmentative method for fiber sphere separating medium
  • Channel augmentative method for fiber sphere separating medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Add 50g cellulose xanthate viscose (containing cellulose 4.1g, CS 2 1.5g, NaOH 3.1g, viscosity is 6800cSt) and 1g concentration is the gelatinized tapioca starch of 5%, stir at room temperature for 20 minutes; Warm up to 30 ℃ and stir for half an hour; Add 300g of mixed oil phase prepared by pump oil and chlorobenzene (pump oil: chlorobenzene=5:1, mass ratio) and 2ml oleic acid, adjust the stirring speed to 500 rpm, keep the speed constant, suspend and disperse at 30°C for 45 minutes, heat up to 90°C within 15 minutes, keep warm After 1.5 hours, solidify to obtain white microspheres; filter the microspheres from the oil phase, wash with boiling water 5 times, add 2% amylase solution of 1 times the volume of the microspheres after cooling, react on a shaking table for 1 hour, and wash with water; The ethanol solution containing 30% acetic acid was regenerated for 1 hour, and then washed with 2 times the volume of methanol and a large amount of water to obtain about 34ml ...

Embodiment 2

[0038] Add 50g cellulose xanthate viscose (containing cellulose 4.1g, CS 2 1.5g, NaOH 3.1g, viscosity is 6800cSt) and 10g concentration are 5% gelatinized tapioca starch, stir at room temperature for 20 minutes; Warm up to 30°C and stir for half an hour; Add 300g of mixed oil phase prepared by pump oil and chlorobenzene (pump oil: chlorobenzene=5:1, mass ratio) and 2ml oleic acid, adjust the stirring speed to 500 rpm, keep the speed constant, suspend and disperse at 30°C for 45 minutes, heat up to 90°C within 15 minutes, keep warm After 1.5 hours, solidify to obtain white microspheres; filter the microspheres from the oil phase, wash with boiling water 5 times, add 2% amylase solution of 1 times the volume of the microspheres after cooling, react on a shaking table for 1 hour, and wash with water; The ethanol solution containing 30% acetic acid was regenerated for 1 hour, and then washed with 3 times the volume of methanol and a large amount of water to obtain about 38ml of ma...

Embodiment 3

[0040] Add 50g cellulose xanthate viscose (containing cellulose 4.1g, CS 2 1.5g, NaOH 3.1g, viscosity is 6800cSt) and 5g concentration is the gelatinized cornstarch of 8%, stir 20 minutes at room temperature; Warm up to 30 ℃ and stir for half an hour; Add 300g mixed oil phase prepared by pump oil and chlorobenzene (pump oil: chlorobenzene=5:1, mass ratio) and 2ml oleic acid, adjust the stirring speed to 500 rpm, keep the speed constant, suspend and disperse at 30°C for 45 minutes, heat up to 90°C within 15 minutes, keep warm After 1.5 hours, solidify to obtain white microspheres; filter the microspheres from the oil phase, wash with boiling water 5 times, add 2% amylase solution of 1 times the volume of the microspheres after cooling, react on a shaking table for 1 hour, and wash with water; The ethanol solution containing 30% acetic acid was regenerated for 1 hour, and then washed with 2 times the volume of methanol and a large amount of water to obtain about 36ml of macropor...

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Abstract

The invention discloses a pore channel expanding method for a cellulose sphere separated substrate, with steps of: 1) preparing compound water phase by mixing gelling starch solution and cellulose xanthate viscose and agitating the same uniformly; 2) thermosetting to form a ball shape by combining an oil phase and a surfactant to form an inverse suspension disperse system, and rising the temperature; 3) removing starch by washing the solidified microsphere by boiled water for five times, adding amylase solution after cooled, then table separating, and water washing; 4) regenerating to obtain the sphere separated substrate by washing the microsphere using organic solvent and regenerating via acid, thus a macropore cellulose sphere separated substrate is obtained. The cellulose sphere separated substrate of the invention has characteristics of large aperture and high pore-size distribution, in addition to advantages of good hydrophilicity, degree of sphericity, acid and alkali resistance and low cost. A test experiment shows that the substrate is applicable to biological macromolecule chromatographic separation medium.

Description

technical field [0001] The invention relates to a pore expansion method of a cellulose spherical separation matrix. Background technique [0002] The rapid development of biotechnology and industrialization has put forward higher and higher requirements for the bioseparation process. Biotechnology products are often of high purity and have special requirements for impurity components, while maintaining biological activity as much as possible. Chromatography is one of the most efficient methods for the separation of biological macromolecules. It is often used in the fine purification process to produce some high value-added biotechnology products. The key to chromatographic separation is the chromatographic medium, which determines the separation efficiency and production cost. At present, chromatographic separation media are mostly made of porous materials to increase the specific surface area and improve the adsorption capacity. [0003] Cellulose is one of the cheapest a...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30
Inventor 林东强夏海锋姚善泾
Owner ZHEJIANG UNIV
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