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Detecting method for fusarium oxysporum pathogenicless strain

A non-pathogenic technology of Fusarium oxysporum, applied in the field of detection of non-pathogenic strains of Fusarium oxysporum, can solve the problems of reduced protection and loss of competitiveness, etc.

Inactive Publication Date: 2007-09-19
BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

[0004] However, as the interference between microorganisms, it often changes due to the influence of various factors. For example, the non-pathogenic Fusarium oxysporum will restore its pathogenicity and lose its competitiveness, resulting in a decrease in protective effect. Therefore, in large areas In terms of application, a set of detection technology must be established to effectively identify non-pathogenic strains of Fusarium oxysporum, so that it can better utilize the disease resistance mechanism of the induced host of non-pathogenic Fusarium oxysporum to prevent plant wilting sick

Method used

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  • Detecting method for fusarium oxysporum pathogenicless strain
  • Detecting method for fusarium oxysporum pathogenicless strain
  • Detecting method for fusarium oxysporum pathogenicless strain

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Embodiment Construction

[0043] 1. Detection steps of Fusarium oxysporum isoenzymes

[0044] The tested strains and sources are shown in Table 1. For the collection of bacteria, after culturing each strain for 7 days, the mycelia were collected, rinsed repeatedly with distilled water, finally pressed dry with filter paper, and stored at -20°C for later use. For sample preparation, the mycelia were placed in a mortar, mixed with 10×Tris-glycine buffer (pH8.3) at a ratio of 1:2 (W / V), ground in an ice bath, refrigerated and centrifuged at 12000rpm for 20min, and taken The supernatant was mixed with an equal volume of 40% sucrose solution and stored in a freezer at -20°C.

[0045] Peroxidase (POD) isoenzyme electrophoresis, (1) Gel preparation: polyacrylamide gel electrophoresis, 4% stacking gel, 7% separating gel. (2) Sample application: use a sampler to apply samples, the loading volume of each sample is 100 μL, and 0.01% bromophenol blue is used as the front indicator. (3) Electrophoresis: After add...

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Abstract

The invention discloses a detecting method of avirulent strains of fusarium, which is characterized in including following steps: (a) detecting isozyme of fusarium; (2) detecting beta-glucosaccharase of fusarium; (c) sequence detecting ITS of fusarium; (d) detecting liquid culture of fusarium, the detecting method can discriminate avirulent strains of fusarium effectively to use thereof as immuno inoculation dose for curepreventing fusarium wilt.

Description

technical field [0001] The invention relates to a method for detecting non-pathogenic strains of Fusarium oxysporum. Background technique [0002] Fusarium wilt is a general term for a class of plant diseases that cause plants to die and wilt due to the attack of pathogenic bacteria. Fusarium wilt has two kinds of bacterial pathogens (such as banana bacterial wilt, etc.) and fungal pathogens. Fusarium wilt referred to in the present invention is a fungal pathogen, a worldwide fungal soil-borne disease caused by the parasitism of Fusarium fungi, wherein Fusarium oxysporum Schl. is one of the main pathogenic species. Fusarium oxysporum genus Fungi imperfecti, Moniliales, Tubercular, Fusarium. Since Snyder and Hansen (1940) put the species in the beautiful group of Fusarium Merge into 1 and become Fusarium oxysporum. Host range: (1) Economic crops: cotton, tung oil, castor, flax, etc.; (2) Solanaceae crops: tomato, pepper, eggplant, tobacco, potato, etc.; (3) Melon crops: cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/34C12Q1/68G01N27/447G01J3/00
Inventor 刘波朱育菁蓝江林肖荣凤林抗美郑雪芳曹宜苏明星史怀葛慈斌阮传清刘芸
Owner BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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