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Method for testing lactose hydrolysis ratio in low lactose milk

A technology for low-lactose milk and a determination method, which is applied in the field of determination of lactose hydrolysis rate in low-lactose milk, can solve the problems of high cost, poor accuracy, and difficult detection and analysis applications, and achieve the effects of low cost, high accuracy, and fast speed

Active Publication Date: 2007-09-12
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the HPLC method is based on a chromatographic instrument. Its biggest advantage is that the accuracy of the lactose hydrolysis rate is high, but the disadvantage is that the cost of each start-up is very high, and even large-scale dairy companies cannot accept this method; while others Several methods are also due to the need for special instruments and related supporting reagents, or because the detection and analysis cycle is too long and the accuracy of the measured results is poor, it is difficult to use these methods in actual practice. It is applied in the detection and analysis, and these methods for detecting the lactose hydrolysis rate of low-lactose milk must have a control sample, that is, the unhydrolyzed milk must be retained so that it can be used to determine the lactose concentration in the milk when the lactose hydrolysis rate is not hydrolyzed. It is not possible for finished product inspection

Method used

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  • Method for testing lactose hydrolysis ratio in low lactose milk
  • Method for testing lactose hydrolysis ratio in low lactose milk
  • Method for testing lactose hydrolysis ratio in low lactose milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A. Determination of the amount of hydrolysis of lactose in low-lactose liquid milk:

[0027] (1) Preparation of sample A: Take 1ml of lactose hydrolyzed milk, add 1ml of carrez reagent A and 1ml of carrez reagent B to precipitate protein, add 7ml of distilled water to dilute to 10 times, oscillate and let stand for about 10 minutes, then filter, and the filtrate is called sample A;

[0028] (2) Pipette 20 μL of 1g / L glucose standard solution and 20 μL of sample A into the working solution mixed with equal volumes of enzyme reagent and phenol reagent;

[0029] (3) After that, place it in a constant temperature water bath at 30°C, react for 30 minutes, measure the absorbance values ​​of the glucose standard solution and the sample at a wavelength of 500nm, and calculate the concentration of glucose in the filtrate:

[0030]

[0031] The concentration of this glucose is the concentration of glucose released by hydrolyzed lactose, so the concentration of hydrolyzed lact...

Embodiment 2

[0045] A. Determination of the amount of hydrolysis of lactose in low-lactose liquid milk:

[0046] (1) Preparation of sample A: take 10ml of lactose hydrolyzed milk, add 10ml of carrez reagent A and 10ml of carrez reagent B to precipitate protein, add 970ml of distilled water to dilute to 100 times, oscillate and let stand for about 20 minutes, then filter, and the filtrate is called sample A;

[0047] (2) Pipette 50 μL of 1g / L glucose standard solution and 50 μL of sample A into the working solution mixed with equal volumes of enzyme reagent and phenol reagent;

[0048] (3) After that, place it in a constant temperature water bath at 40°C, react for 15 minutes, measure the absorbance values ​​of the glucose standard solution and the sample at a wavelength of 520nm, and calculate the concentration of glucose in the filtrate:

[0049]

[0050] The concentration of this glucose is the concentration of glucose released by hydrolyzed lactose, so the concentration of hydrolyze...

Embodiment 3

[0064] A. Determination of lactose hydrolysis amount after hydrolysis of low-lactose milk powder:

[0065] (1) Preparation of sample A: Take 10g of milk powder and dilute it to a 100mL volumetric flask to make reduced milk powder, then take 5mL of reduced milk powder, add 5mL of carrez reagent A and 5mL of carrez reagent B to precipitate protein, then add 235mL of distilled water to dilute to 50 times, After oscillating, let stand for about 15 minutes, then filter, and the filtrate is sample A;

[0066] (2) Pipette 30 μL of 1g / L glucose standard solution and 30 μL of sample A into the mixed working solution of equal volumes of enzyme reagent and phenol reagent;

[0067] (3) After that, place it in a constant temperature water bath at 35°C, react for 20 minutes, measure the absorbance values ​​of the glucose standard solution and the sample at a wavelength of 510nm, and calculate the concentration of glucose in the filtrate:

[0068]

[0069] The concentration of this gluco...

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Abstract

This application discloses a glucose oxidase - spectrophotometric method of measuring low lactose creamery's lactose hydrolization rate. this method includes (1) determine lactose concentration after creamery hydrolization; (2) determine lactose concentration before creamery hydrolization;(3) calculate lactose hydrolization rate, hydrolyze rate =( lactose concentration after hydrolization / lactose concentration before hydrolization)X100%. This test method has feature of fast velocity, low cost, high accuracy.

Description

technical field [0001] The invention relates to a method for measuring the hydrolysis rate of lactose in low-lactose milk, more specifically, a method for measuring the hydrolysis rate of lactose in low-lactose milk by using the glucose oxidase-spectrophotometric method described in this application. Background technique [0002] Low-lactose milk uses lactase to hydrolyze lactose in milk to produce glucose and galactose. Therefore, the hydrolysis rate is an important production index to measure the degree of hydrolysis of low-lactose milk. The methods of measuring the hydrolysis rate of low-lactose milk introduced in the literature data report mainly are high-performance liquid chromatography (HPLC) (Gan Binbin, 2001), freezing point depression method (Ma Fuxia, 2002), iodometric method (Qin Lihu, 2002) 2003) and several others. Among them, the HPLC method is based on a chromatographic instrument. Its biggest advantage is that the accuracy of...

Claims

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Application Information

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IPC IPC(8): G01N21/78G06F19/00
Inventor 卜建斌云战友
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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