Prepn process of collagen-based surface wound repairing membrane possessing tissue induction

A wound repair and inductive technology, applied in bandages, absorbent pads, medical science and other directions, can solve the problems of thin and fragile membranes, lack of dermal components, poor mechanical properties, etc., to avoid scarring, shorten wound healing time, Well-integrated effect

Inactive Publication Date: 2007-08-22
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

①Epidermal cell membrane: The disadvantage of this membrane (KC) is that the patient’s skin biopsy specimen must be taken, which requires 2-3 weeks of preparation time, lacks dermal components, and the membrane is thin and fragile [O'ConnerNE, Malliken JB, Bankaclilegcls, et al al. Grafting of burns with cultured epitheliunt prepared from autologous epidermal cells [J]. Lancet, 1981, 1: 75-78]
② Acellular dermal matrix: its disadvantage is that it may contain unclean antigenic substances, which may cause the risk of virus infection, and the material has poor pore penetration
③ Collagen sponge membrane: such as Chinese patents 94118836.1 and 01134743.0, its disadvantage is that the material does not contain cell growth factors, and its mechanical properties are poor, and it cannot resist infection; Chinese patent 200510022581. Microsphere slow-release technology, growth factors are easy to lose biological activity
④ Collagen gel: such as Chinese patents 02117585.3 and 03130382.X, the disadvantage is that the production process is complicated, the collagen gel will shrink by about 80%, the ability to resist collagenase degradation is poor, and it is easy to be infected by viruses and immune rejection. difficult to operate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Preparation of sustained-release microspheres

[0029] 50 parts of castor oil containing 1% Tween-80 is placed in a three-necked bottle, preheated to 50° C., and under stirring, 5 parts of gelatin with a total concentration of 5%: chitosan=9: 1 mixed solution are added dropwise, Stir for 10 minutes to form a water-in-oil emulsion and use an ice bath instead, continue stirring for 10 minutes, add 30 parts of acetone to the emulsion, stir the emulsion for 30 minutes, add 3 parts of 1% glutaraldehyde, and pre-modify for 1 hour. The obtained gelatin-based microspheres were washed twice with acetone and twice with isopropanol, collected by centrifugation (10000rmp, 15min, 4°C), and air-dried. Then immerse the gelatin microspheres in a solution containing 0.25% glutaraldehyde and 1% Tween-80, stir at a temperature of 4°C for 12 hours, and centrifuge to separate the obtained microspheres into a solution containing 10mM glycine and 1% Tween-80. In the solution, stir at 4°C ...

Embodiment 2

[0033] 1. Preparation of sustained-release microspheres

[0034] Put 80 parts of liquid paraffin containing 2% Span-80 in a three-necked flask, preheat to 50°C, add 8 parts of gelatin:collagen=8:2 mixed solution with a total concentration of 8% dropwise under stirring, and stir to form oil After the water-wrapped emulsion, switch to an ice bath and continue stirring for 10 minutes. Add 40 parts of acetone to the emulsion, stir the emulsion for 30 minutes, add 2 parts of 3% glutaraldehyde, and pre-modify for 1 hour. The obtained gelatin-based composite microspheres were washed twice with acetone and twice with isopropanol, collected by centrifugation (10000rmp, 15min, 4°C), and air-dried to obtain uncrosslinked microspheres. Immerse uncrosslinked gelatin-based composite microspheres in a solution containing 0.5% glutaraldehyde and 1% Span-80, stir at a temperature of 4°C for 12 hours, and centrifuge to separate the obtained microspheres into a solution containing 20mM glycine ...

Embodiment 3

[0038] 1. Preparation of sustained-release microspheres

[0039] Put 100 parts of olive oil containing 3% Span-80 in a three-necked bottle, preheat to 50°C, and add dropwise a total concentration of 10% gelatin: collagen: chitosan=8:1:1 mixed solution under stirring 20 parts, after stirring to form a water-in-oil emulsion, use an ice bath instead, and continue stirring for 10 minutes. Add 50 parts of acetone to the emulsion, stir the emulsion for 30 minutes, add 1 part of 5% glutaraldehyde, and pre-modify for 1 hour. The obtained gelatin-based composite microspheres were washed twice with acetone and twice with isopropanol, collected by centrifugation (10000rmp, 15min, 4°C), and air-dried to obtain uncrosslinked microspheres. Immerse the uncrosslinked gelatin-based composite microspheres in a solution containing 1% glutaraldehyde and 1% Span-80, stir for 12 hours at a temperature of 4°C, and centrifuge to separate the obtained microspheres into a solution containing 50mM glyc...

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Abstract

The present invention is preparation process of collagen-based surface wound repairing membrane possessing tissue induction function, and features that the collagen-based surface wound repairing membrane modified physically and chemically has introduced gelatin-base slow releasing microsphere containing cell growth factor, pores of 20-200 micron size, pore rate not smaller than 80 %, tensile strength not smaller than 1 MPa, pH 5.0-6.0, and excellent degradation and biocompatibility. It has the functions of absorbing wound diffusate, induce the adhesion, proliferation and differentiation of autocell, induce the growth of blood vessel, release cell growth factor to the wound for long time, speed wound healing and avoid scar. The present invention is used for injury, burns, scalds, skin ulcer, etc.

Description

technical field [0001] The invention relates to a preparation method of a collagen matrix surface wound repair membrane material with tissue induction, belonging to the field of preparation of medical materials. Background technique [0002] Skin is the natural barrier of the human body, which plays an important role in maintaining the stability of the internal environment and preventing microbial invasion. If the skin is damaged in a large area, it will cause many local or even systemic problems, such as increased metabolism, excessive loss of water and protein, immune system disorders, etc., and even life-threatening. Reconstructing or restoring the skin barrier function is the ultimate goal of body surface wound therapy. Before this goal is achieved, a wound dressing with excellent performance can temporarily function as part of the skin and provide an environment conducive to wound healing, waiting for the epithelialization of the wound or Transition to rebuilding the p...

Claims

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Application Information

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IPC IPC(8): A61L15/32
Inventor 但卫华叶易春曾睿关林波
Owner SICHUAN UNIV
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