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Method for checking bacterial number after filtering using filtering film

A post-filtration and membrane technology, applied in the field of microbial detection, to solve the problem of unrealistic film, reduce manpower, material resources and time costs, and reduce pollution problems

Inactive Publication Date: 2007-08-01
叶大林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] It can be seen from the above that the current methods all have certain defects. A simpler and more reliable method is needed to check the number of bacteria after membrane filtration, so as to facilitate the recovery and growth of microorganisms, reduce detection deviation, and improve accuracy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare a microporous filter membrane with a pore size of 0.45 μm and a diameter of 60 mm, and pre-fix it on the filter membrane support unit in the filter to make a special negative pressure filter (the structural components of the filter used to support the filter membrane are detachable ), after sterilized, connect it with the matching negative pressure suction filter joint. Take a certain amount of sample and transfer it to the filter cup for filtration. After the completion, separate the filter from the negative pressure suction filter joint, close the opening at the upper end of the filter cup, turn the filter upside down, and expose the filter membrane. Pour the nutrient agar medium into the filter membrane so that the surface is completely covered by the medium. After completion, cover it with a cover that matches it, let it cool and solidify naturally, and culture it at 35°C, and count the colonies after cultivation.

Embodiment 2

[0029] Prepare a microporous filter membrane with a pore size of 0.45 μm and a diameter of 75 mm, which is pre-fixed on the upper end of the filter membrane support member of the closed positive pressure filter to make a special positive pressure filter (the filter is used to support the filter membrane) The structural components are detachable), and after sterilized, it is assembled and connected with a special peristaltic pump. Take a certain amount of sample, transfer it to the filter through the elastic hose connected to the peristaltic pump and carry out pressure filtration. Membrane support member to expose the filter membrane, directly pour the unsolidified nutrient agar medium not exceeding 45°C on the filter membrane, cover it with a matching cover after completion, let it cool and solidify naturally, and place it at 35°C for cultivation , colony count after incubation.

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PUM

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Abstract

The invention discloses a method for counting bacteria after filtering with filter membrane. It comprises following steps: filtering sample on filter membrane to make the bacteria in sample be cutted off on filter membrane, pouring solid or semi- solid culture medium on the back surface of bacteria- containing filter membrane, culturing, and colony counting. The temeprature of said solid pr semi- solid culture medium is not larger than 45 Deg. C, and it is in flow state during pouring. The invention is characterized in that it is unecessary for filter membrane to be shifted after filtering, which reduces pollution caused during filter membrane shift and obviously increases result accuracy; it avoids bubble generation and space between filter membrane and culture medium, and free from influence of planeness of filter membrane and operational technology; solves problems of unstable pad pasting and slide filter membrane, and greatly reduces manual effort, material resources and time.

Description

technical field [0001] The invention relates to a method for detecting microorganisms, in particular to a method for checking the number of bacteria after membrane filtration. Background technique [0002] As a method of microbial detection, membrane filtration method is to fix the microporous filter membrane on a special filter, filter under certain positive or negative pressure conditions, and use the tiny pore size of the microporous filter membrane to separate liquid and microorganisms Separation, microorganisms are retained on the surface of the filter membrane, and then the filter membrane is contacted with the medium, and cultured under specified conditions. Because the filter membrane has water absorption function and is on the surface of the culture medium, the nutrients in the culture medium will continuously penetrate into the filter membrane with the water, and the single bacterial cells retained on the filter membrane will thus obtain nutrition. Growth was resu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
Inventor 叶大林
Owner 叶大林
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