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Method for investigating quality of characterization natural cordyceps sinensis character

A technology for quality evaluation of Cordyceps sinensis, applied in the field of Cordyceps quality evaluation to characterize the characteristics of natural Cordyceps sinensis, can solve the problems of insufficient quality evaluation methods of Cordyceps, affecting the safety and effectiveness of Cordyceps quality evaluation, etc.

Inactive Publication Date: 2007-07-18
李绍平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The 2005 edition of "(Chinese Pharmacopoeia) stipulates that the content of adenosine in natural Cordyceps sinensis should not be less than 0.01% (0.1mg / g), but literature reports and our research have proved that the content of adenosine in artificial Cordyceps sinensis is much higher than that in natural Cordyceps sinensis, Tip: There are obvious deficiencies in the quality evaluation method of Cordyceps using adenosine as an index
Although many methods have been reported for the determination of various nucleoside components in Cordyceps sinensis, there is no nucleoside component index and determination method that can characterize the characteristics of natural Cordyceps sinensis, which seriously affects the quality evaluation of Cordyceps sinensis and the safety of its application. and validity

Method used

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  • Method for investigating quality of characterization natural cordyceps sinensis character
  • Method for investigating quality of characterization natural cordyceps sinensis character

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Sample preparation: Take about 0.5g of natural Cordyceps sinensis sample powder into a 50ml stoppered tube, ①accurately add 10ml of deionized water, weigh it, and mix well. After standing at room temperature for 18 hours, sonicate for 30 minutes, make up the weight, and centrifuge; ②Add 10ml of methanol, weigh, mix, ultrasonicate for 30 minutes, make up the weight, and centrifuge; the supernatant is filtered with a 0.45 μm filter membrane and used for HPLC analyze.

[0037] HPLC analysis: Agilent 1100 series high performance liquid chromatograph, equipped with quaternary pump, automatic sampling system, diode array detector (DAD) and Agilent chromatographic workstation. The chromatographic column is ZorbaxSB-C18 (250×4.6mm i.d., 5μm) analytical column and Zorbax SB-C18 (12.5×4.6mm i.d., 5μm) pre-column. The elution condition is water (containing 10 mM triethylamine) (A) and methanol (B) gradient elution: 0 to 30 minutes, gradient elution from 0% B to 30% B. The detect...

Embodiment 2

[0040]Sample preparation: Take about 0.5g of the artificial Cordyceps sample powder into a 50ml stoppered tube, ①accurately add 10ml of deionized water, weigh it, and mix well. After standing at room temperature for 18 hours, sonicate for 30 minutes, make up the weight, and centrifuge; ②Add 10ml of methanol, weigh, mix, ultrasonicate for 30 minutes, make up the weight, and centrifuge; the supernatant is filtered with a 0.45 μm filter membrane and used for HPLC analyze.

[0041] HPLC analysis: with embodiment 1.

[0042] Measurement results, refer to Figure 3 and Figure 4: Determination of the content ratio of uridine, inosine, guanosine, adenosine and cordycepin in 9 batches of artificial Cordyceps taken from Jiangxi, Hebei, Zhejiang and Anhui, etc. The detection data of the warm water ultrasonic treatment sample is: 100:0~20:40~160:30~140:0~10, wherein the uridine content is 1.0~4.0mg / g; the detection data of the methanol treatment sample is: 100:0 ~20:80~180:60~160:0~10, w...

Embodiment 3

[0044] Sample preparation: Take about 0.5 grams of artificial Cordyceps militaris sample powder into a 50ml stoppered tube, ①accurately add 10ml of deionized water, weigh it, and mix well. After standing at room temperature for 18 hours, sonicate for 30 minutes, make up the weight, and centrifuge; ②Add 10ml of methanol, weigh, mix, ultrasonicate for 30 minutes, make up the weight, and centrifuge; the supernatant is filtered with a 0.45 μm filter membrane and used for HPLC analyze.

[0045] HPLC analysis: with embodiment 1.

[0046] For the measurement results, refer to Figure 5 and Figure 6: the content ratios of uridine, inosine, guanosine, adenosine and cordycepin in 7 batches of artificial Cordyceps militaris collected from Jiangsu, Shanghai, Guangdong and Liaoning, etc. were measured, The detection data of the room temperature water ultrasonic treatment sample is 100:0~10:20~100:50~90:200~800, wherein the uridine content is 1.3~3.1mg / g; the detection data of the methanol ...

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Abstract

The invention relates to nature caterpillar fungus quality assessment method. It includes the following steps: using organic solvent or water with over 60 degree centigrade to extract sample; processing HPLC analysis for the extracted liquid to gain random three component content and proportion of the uridine, inosine, guanosine, adenosine, and cordycepin as detected result A; using water with under 60 degree centigrade to extract sample; processing HPLC analysis for the extracted liquid to gain their content and proportion as the detected result B; comparing detected result A and B with characterization index; if both of the A and B are in the range of the characterization index, the sample has the same quality characteristics with the caterpillar fungus. The invention objectively confirms the detecting method and index for the nature caterpillar fungus characteristics.

Description

technical field [0001] The invention relates to a quality evaluation method for Cordyceps sinensis (including natural Cordyceps sinensis, artificial Cordyceps sinensis and artificial Cordyceps militaris), in particular to a quality evaluation method for characterizing the characteristics of natural Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is an entomogenic fungus that parasitizes the larvae of bat insects of the order Lepidoptera. It is a traditional Chinese medicine that has the functions of nourishing the kidney and benefiting the lungs. In my country, Cordyceps sinensis is mainly distributed in Tibet, Qinghai, Sichuan, Yunnan, Gansu and other provinces and regions with an altitude of 3000-5000m, near the alpine snow line such as meadows, shrubs, hillsides, valleys, etc. The Qinghai-Tibet Plateau is a high-yielding area. Due to the important edible and medicinal value of Cordyceps sinensis, the wild resources are extremely collected, resulting i...

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N1/28
Inventor 李绍平杨丰庆
Owner 李绍平
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