Transplantation method for nucleus
A cell nucleus and oocyte technology, applied in the field of cell engineering, can solve the problems of wasting too much time, inaccurate, and removing the target nucleus and cytoplasm, and achieve the effects of improving the survival rate, reducing time and easy operation.
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Embodiment 1
[0047] (1) Use an injection needle with a diameter of 8 μm to absorb granule cells, repeatedly blow and blow the cells to rupture the cell membrane, and then suck them into the injection needle. leave appropriate gaps;
[0048] (2) Get the mice that have undergone superovulation treatment, kill them by necking, and take out the oviducts;
[0049] (3) Pull out the oocytes with granulosa cells in the ampulla of the oviduct with a fine needle, then put them into the culture medium containing hyaluronidase, and treat them in a 37°C incubator for 5 minutes to completely remove the granulosa cells;
[0050] (4) Take oocytes with complete polar bodies in good condition and fix them in the direction of 9 o'clock with a 15 μm ovum-holding needle (Fig. 1);
[0051] (5) Move the tip of the injection needle on the other side to directly below the oocyte along the opposite direction of the egg-holding needle (Figure 2);
[0052] (6) Move the injection needle upwards, squeeze the oocytes ...
Embodiment 2
[0060] (1) Use an injection needle with a diameter of 8 μm to absorb granule cells, repeatedly blow and blow the cells to rupture the cell membrane, and then suck them into the injection needle. leave appropriate gaps;
[0061] (2) Get the mice that have undergone superovulation treatment, kill them by necking, and take out the oviducts;
[0062] (3) Pull out the oocytes with granulosa cells in the ampulla of the oviduct with a fine needle, then put them into the culture medium containing hyaluronidase, and treat them in a 37°C incubator for 5 minutes to completely remove the granulosa cells;
[0063] (4) Take oocytes with complete polar bodies in good condition and fix them in the direction of 9 o'clock with a 20 μm ovum-holding needle (Fig. 1);
[0064] (5) Move the tip of the injection needle on the other side to directly below the oocyte along the opposite direction of the egg-holding needle (Figure 2);
[0065] (6) Move the injection needle upwards, squeeze the oocytes ...
Embodiment 3
[0073] (1) Use an injection needle with a diameter of 8 μm to absorb granule cells, repeatedly blow and blow the cells to rupture the cell membrane, and then suck them into the injection needle. leave appropriate gaps;
[0074] (2) Get the mice that have undergone superovulation treatment, kill them by necking, and take out the oviducts;
[0075] (3) Pull out the oocytes with granulosa cells in the ampulla of the oviduct with a fine needle, then put them into the culture medium containing hyaluronidase, and treat them in a 37°C incubator for 5 minutes to completely remove the granulosa cells;
[0076] (4) Take oocytes with complete polar bodies in good condition and fix them in the direction of 9 o'clock with a 30 μm ovum-holding needle (Fig. 1);
[0077] (5) Move the tip of the injection needle on the other side to directly below the oocyte along the opposite direction of the egg-holding needle (Figure 2);
[0078] (6) Move the injection needle upwards, squeeze the oocytes ...
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