35results about How to "Rapid early identification" patented technology

The invention provides a molecule specificity marker primer of the improved variety of camellia oleifera Changlin Number 55. The sequence of the primer is that the upstream primer is 5'-CATACATACACTTCCTAAGCCAAAA-3' and the upstream primer is 5'-GTTCAAGCATTGTTCAAGCACTC-3'. The molecule specificity marker primer can be used for the early-stage identification of the improved variety of camellia oleifera Changlin Number 55. The method is simple, rapid and accurate and is a molecular method which can not be replaced by distinguishing the improved variety of camellia oleifera by appearance characteristics.

The invention discloses a molecular-specificity labeling primer for oil-tea good-variety longlin 18 with high specificity and a method capable of carrying out rapid identification on the oil-tea good-variety longlin 18. The sequence of the primer is as follows: the upstream primer is 5'-AGCGGCCGCGTAATAAGTCTAAGC-3', and the downstream is 5'-AGCGGCCGCGTTGGATCA-3'. The molecular-specificity labeling primer can be used for carrying out rapid and early identification on the oil-tea good-variety longlin 18, is simple, fast and accurate and is a molecular means which can not be replaced for identifying the good oil-tea variety by apparent characteristics.

The invention relates to a molecule specificity labeling primer for camellia oleifera improved varieties changlin number three and twenty one and a method capable of fast identifying the camellia oleifera improved varieties changlin number three and twenty one. The primer has the following sequence of an upstream primer body 5'-AATGAATGGTGCTTTGGATT-3' and a lower primer body 5'-CAAAACCACACCTTGAGTCC-3'. The molecule specificity labeling primer can fast identify the camellia oleifera improved varieties changlin number three and twenty one at the early stage, and the method is simple, fast and accurate and is an irreplaceable molecule means for identifying camellia oleifera improved varieties through the apparent characteristics.

The invention relates to a pair of high-specificity molecular specific labeled primers of an oil-tea camellia fine breed Changlin No.21 and a rapid identification method of oil-tea camellia fine breed Changlin No.21. The primers have the sequences as follows: a forward primer: 5'-TCACTCTTCAGCAGCTACCCAA-3 ', and a downstream primer: 5'-CCTTCTCTATCGCTCTCAATCTCG-3'. The molecular specific labeled primers can conduct quick early identification on oil-tea camellia fine breed Changlin No.21; the method is simple, fast and accurate, and is an irreplaceable molecular tool for discrimination of oil-tea camellia fine breed by apparent characteristics.

The invention discloses a primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus. The primer pair comprises an upstream primer and a downstream primer, wherein the upstream primer is 5'-ATTAGCTTGACGTACAGACAGTCG-3', and the downstream primer is 5'-TGTTGGAGCAAGGGTGATACGA-3'. According to the primer pair, hericium erinaceus houjie No.2 and large hericium erinaceus share one specific molecular marker. The PCR method adopting the primer pair has the advantages that detection time is short, accuracy is high, judgment is easy and repeatability is high when used for identifying hericium erinaceus houjie No.2 or large hericium erinaceus, and early-stage identification can be achieved quickly.

The invention discloses a primer pair and method for identifying hericium erinaceus houjie No.2. The primer pair comprises an upstream primer and a downstream primer, wherein the upstream primer is 5'-CAAACCGGTGTCAGGGAACTGC-3', and the downstream primer is 5'-ACGAATTTGAATCCTCCACGTCGAA-3'. The primer pair is obtained through hericium erinaceus houjie No.2 specific molecular marker design. The primer pair has the advantages that detection time is short, accuracy is high, judgment is easy and repeatability is high when used for identifying hericium erinaceus houjie No.2 with the PCR method, and early-stage identification can be achieved quickly.

The invention relates to a high-specificity molecular specific labeling primer for oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose' and a method capable of rapidly identifying the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'. The sequences of the specific labeling primer are as follows: the sequence of an upstream primer is 5'-AGCGGCCGCTAAGCAG-3', and the sequence of a downstream primer is 5'-AGCGGCCGCTACCTTTAC-3'. The molecular specific labeling primer can be used for carrying out rapid early phase identification on the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'; the method is simple, rapid and accurate and is an irreplaceable molecular measure for distinguishing the oriental cherry varieties from apparent characteristics.

The invention relates to a pair of molecularly specific labeling primers of a sakura variety 'Hirano Moseu' having high specificity and a method capable of rapidly identifying the sakura variety 'Hirano Moseu'. The sequences of the primers are as follows: a forward primer: 5'-AGCGGCCGCATATGGATTGTTACC-3' and a reverse primer: 5'-GGCCGCATCGGGCGAAGA-3'. By the molecularly specific labeling primers, the sakura variety 'Hirano Moseu' can be rapidly and early identified and the method is simple, fast and accurate and is an irreplaceable molecular means for apparently characterizing and identifying the sakura variety.

The invention relates to a molecular specific marker primer for Hibiscus syriacus varieties (Hibiscus syriacus OiseauBleu, Hibiscus syriacus Floru, Hibiscus syriacus Ardens and Hibiscus syriacus Minrosa) and an assay method, which are used for identifying the Hibiscus syriacus varieties. The application of the Hibiscus syriacus varieties to gardens is extremely wide, but the varieties can hardly be identified from the aspect of phenotypic characteristics. In the invention, the sequences of the molecular specific marker primer for Hibiscus syriacus varieties (Hibiscus syriacus OiseauBleu, Hibiscus syriacus Floru, Hibiscus syriacus Ardens and Hibiscus syriacus Minrosa) are: forward primer: 5'-AAGATACACTGGAATAGAACA-3'; and reverse primer: 5'-GAATTCACGCTTCGATGCTCA-3'. The molecular specific marker primer pair can be utilized for carrying out rapid early identification on the Hibiscus syriacus varieties, i.e., the Hibiscus syriacus OiseauBleu, Hibiscus syriacus Floru, Hibiscus syriacus Ardens and Hibiscus syriacus Minrosa, and the method is simple, highly efficient and accurate, and is an irreplaceable molecular means for identifying the Hibiscus syriacus varieties from the aspect of apparent characteristics.

The invention discloses a molecular specific marker primer and an identification method for fine varieties of camellia oleifera. Sequences of the molecular specific marker primer include upstream primers 5'-CGGACTGATTATCCTTGGCAAT-3' and downstream primers 5'-AGATCCCGCCCAAGACCTT-3'. The molecular specific marker primer and the identification method have the advantages that the fine varieties NO.21 of the camellia oleifera can be quickly identified by the molecular specific marker primer at early stages, the identification method is simple, speedy and accurate, and the molecular specific marker primer and the identification method are irreplaceable molecular means for discriminating the fine varieties of the camellia oleifera by the aid of appearance features.

The invention relates to a pair of highly specific molecular specific marker primers for Camellia oleifera varieties Changlin 3 and Changlin 21, and a method for quickly identifying the Camellia oleifera varieties Changlin 3 and Changlin 21. The primer sequences are as follows: upstream primer: 5'-AATGAATGGTGCTTTGGATT-3', downstream primer: 5'-CAAAACCACACCTTGAGTCC-3'. The molecular specific marker primer of the present invention can quickly and early identify the Camellia oleifera improved varieties Changlin Changlin 3 and No. 21, the method is simple, quick and accurate, and is an irreplaceable molecular means for distinguishing the improved Camellia oleifera varieties by appearance characteristics.

The invention relates to molecule specificity labeled primers with high specificity for improved tea-oil tree varieties No. 18 and 21 of the Changbu experimental Forestry Center, and a method for quickly identifying the improved tea-oil tree varieties No. 18 and 21 of Changbu Forestry Center. The primer sequences are as follows: the upstream primer: 5'-CTCAAGGCTTCACTAACAGCAA-3', and the downstream primer: 5'-CTGCCTCTCAATCTTGTTCACC-3'. The molecule specificity labeled primers can perform quick early-period identification on the improved tea-oil tree varieties No. 18 and 21 of the Changbu experimental Forestry Center; the method is simple, quick and accurate and is an irreplaceable molecule means for identifying the improved tea-oil tree varieties through morphologic features.

The invention relates to a high-specificity molecular specific labeling primer for an oriental cherry variety namely C.serrulata 'Plena-pendula' and a method capable of rapidly identifying the oriental cherry variety namely C.serrulata 'Plena-pendula'. The sequences of the specific labeling primer are as follows: the sequence of an upstream primer is 5'-AGCGGCCGCGTGGTATGGA-3', and the sequence of a downstream primer is 5'-AGCGGCCGCGTGTTGTAGAAG-3'. The molecular specific labeling primer can be used for carrying out rapid early phase identification on the oriental cherry variety namely C.serrulata 'Plena-pendula'; the method is simple, rapid and accurate and is an irreplaceable molecular measure for distinguishing the oriental cherry varieties from apparent characteristics.

The invention relates to the technical fields of plant genetic engineering and molecular biology, in particular to a molecular detection method for quickly identifying true plum / apricot plum strains. The present invention provides plum blossom PmEIL protein, the sequence of which is shown in SEQ ID NO.1. PmEIL protein is involved in the response of plum blossoms to seasonal climate changes. There are obvious differences in the regulation methods of real plum and apricot plum strains by seasonal climate changes such as temperature and sunshine. Quickly and accurately identify plum blossom strains, shorten the breeding cycle, and greatly reduce the breeding workload. The invention also provides a SNP molecular marker related to the PmEIL gene and related to plum blossom stem color, which provides a basis and a molecular tool for the identification of plum blossom stem color traits and the identification of true plum / apricot plum strains.

The invention relates to molecular distinctness labeling primers for aster dendrobium and a method capable of rapidly identifying aster dendrobium. According to the primers, the sequence of the upstream primer is 5'-AGCGGCCGCGCCTTCC-3', and the sequence of the downstream primer is 5'-GGAAGGCGCGGCCGCT-3'. The molecular distinctness labeling primers for aster dendrobium and the method capable of rapidly identifying aster dendrobium have the advantages that the molecular distinctness labeling primers can carry out rapid early-stage identification on aster dendrobium, and the method is simple, fast and accurate, and is a molecular means which cannot be replaced by the method of distinguishing dendrobium varieties with the apparent characteristics.

The invention relates to a molecular specific marker primer and a detection method for Hibiscus varieties of Hibiscus syriacus 'Bricutts', Hibiscus syriacus 'Notwoodone', Hibiscus syriacus 'Sanchoyo' and Hibiscus syriacus 'Notwoodtwo', which are applied to identification of the Hibiscus varieties. The Hibiscus varieties are widely used in gardens, but are difficult to identify from the aspectof phenotypic characteristics. The molecular specific marker primer sequences of the Hibiscus varieties of Hibiscus syriacus 'Bricutts', Hibiscus syriacus 'Notwoodone', Hibiscus syriacus 'Sanchoyo'and Hibiscus syriacus 'Notwoodtwo' are: upstream primer: 5'-CTCTCTCAACTCTCATCGG-3'; downstream primer: 5'-GGAAAAAAAAAAAACA-AAAG-3'. The molecular specific marker primer can be applied to rapid earlyidentification of the Hibiscus varieties of Hibiscus syriacus 'Bricutts', Hibiscus syriacus 'Notwoodone', Hibiscus syriacus 'Sanchoyo' and Hibiscus syriacus 'Notwoodtwo'. The method is simple, fastand accurate, and is an irreplaceable molecular means for identifying Hibiscus varieties by apparent characteristics.

The invention relates to molecular specificity labeling primers of dendrobium strongylanthum rchb.f. and a method capable of rapid identification of the dendrobium strongylanthum rchb.f. The primers have the sequences: an upstream primer of 5'-AGCGGCCGCGTGGTACAC-3', and a downstream primer of 5'-GTGTACCACGCGGCCGCT-3'. The beneficial effects mainly comprise that the molecular specificity labeling primers can perform rapid early identification on the dendrobium strongylanthum rchb.f.; and the method is simple, fast and accurate, and is an irreplaceable molecular means for apparent characteristic identification of the variety of the dendrobium strongylanthum rchb.f.

The invention discloses a molecular-specificity labeling primer for oil-tea good-variety longlin 18 with high specificity and a method capable of carrying out rapid identification on the oil-tea good-variety longlin 18. The sequence of the primer is as follows: the upstream primer is 5'-AGCGGCCGCGTAATAAGTCTAAGC-3', and the downstream is 5'-AGCGGCCGCGTTGGATCA-3'. The molecular-specificity labeling primer can be used for carrying out rapid and early identification on the oil-tea good-variety longlin 18, is simple, fast and accurate and is a molecular means which can not be replaced for identifying the good oil-tea variety by apparent characteristics.

The invention provides a molecule specificity marker primer of the improved variety of camellia oleifera Changlin Number 55. The sequence of the primer is that the upstream primer is 5'-CATACATACACTTCCTAAGCCAAAA-3' and the upstream primer is 5'-GTTCAAGCATTGTTCAAGCACTC-3'. The molecule specificity marker primer can be used for the early-stage identification of the improved variety of camellia oleifera Changlin Number 55. The method is simple, rapid and accurate and is a molecular method which can not be replaced by distinguishing the improved variety of camellia oleifera by appearance characteristics.

The invention relates to a high-specificity molecular specific labeling primer for oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose' and a method capable of rapidly identifying the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'. The sequences of the specific labeling primer are as follows: the sequence of an upstream primer is 5'-AGCGGCCGCTAAGCAG-3', and the sequence of a downstream primer is 5'-AGCGGCCGCTACCTTTAC-3'. The molecular specific labeling primer can be used for carrying out rapid early phase identification on the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'; the method is simple, rapid and accurate and is an irreplaceable molecular measure for distinguishing the oriental cherry varieties from apparent characteristics.

The invention relates to a pair of high-specificity molecular specific labeled primers of an oil-tea camellia fine breed Changlin No.21 and a rapid identification method of oil-tea camellia fine breed Changlin No.21. The primers have the sequences as follows: a forward primer: 5'-TCACTCTTCAGCAGCTACCCAA-3 ', and a downstream primer: 5'-CCTTCTCTATCGCTCTCAATCTCG-3'. The molecular specific labeled primers can conduct quick early identification on oil-tea camellia fine breed Changlin No.21; the method is simple, fast and accurate, and is an irreplaceable molecular tool for discrimination of oil-tea camellia fine breed by apparent characteristics.