35results about How to "Rapid early identification" patented technology

The invention relates to a molecule specificity labeling primer for the high-specificity tea-oil tree improved variety Chang Lin 18 and a detection method capable of rapidly detecting tea-oil tree improved variety Chang Lin 18. According to the primer sequence, the upstream primer is 5'-AGCCAAAATTGCGTCAAGTCTTCT-3', and the downstream primer is 5'-AGCAAGCAAAACCACACCCCCAA-3'. The molecule specificity labeling primer can carry out rapid early-stage detection on tea-oil tree improved variety Chang Lin 18, and the method is easy, fast and accurate and is the irreplaceable molecular method for presenting characteristics and distinguishing tea-oil tree improved varieties.

The invention relates to a pair of high-specificity molecular specific marker primers for sakura varieties including Superba and Grandifora, and a method for quickly identifying the sakura varieties including Superba and Grandifora. Primer sequences are as follows: an upstream primer: 5'-AGCGGCCGCACAAATTAAGAAAAA-3'; a downstream primer: 5'-AGCGGCCGCACTTTGAACGAT-3'. The molecular specific marker primers can be used for quick preliminary identification of the sakura varieties including Superba and Grandifora, and the method is simple, quick and convenient and is a molecular means irreplaceable by apparent characteristic identification of the sakura varieties.

The invention relates to a pair of high-specificity molecular specific marker primers for sakura varieties including Superba and Grandifora, and a method for quickly identifying the sakura varieties including Superba and Grandifora. Primer sequences are as follows: an upstream primer: 5'-AGCGGCCGCACAAATTAAGAAAAA-3'; a downstream primer: 5'-AGCGGCCGCACTTTGAACGAT-3'. The molecular specific marker primers can be used for quick preliminary identification of the sakura varieties including Superba and Grandifora, and the method is simple, quick and convenient and is a molecular means irreplaceable by apparent characteristic identification of the sakura varieties.

The invention relates to a pair of high-specificity molecularly specific labeling primers for mountain rice Bailinggu No.2 and No.19 which have high specificity and a method capable of rapidly detecting the mountain rice Bailinggu No.2 and No.19. The sequences of the primers are as follows: the sequence of the upstream primer is 5'-AGCGGCCGCATCCTGAGAGA-3' and the sequence of the downstream primer is 5'-AGCGGCCGCATCCTGAGAG-3'. By using the molecularly specific labeling primers, the early identification of mountain rice Bailinggu No.2 and No.19 can be rapidly performed, and the method is simple, fast and accurate and is an irreplaceable molecular tool for distinguishing mountain rice varieties in apparent characteristics.

The invention relates to a pair of high-specificity molecular specific marker primers for sakura varieties including Plena Rosea, Sakuyahime and Ujou-shidare, and a method for quickly identifying the sakura varieties including Plena Rosea, Sakuyahime and Ujou-shidare. Primer sequences are as follows: an upstream primer: 5'-AGCGGCCGCCGCTAGATGAG-3'; a downstream primer: 5'-AGCGGCCGCCGAGACAAAGA-3'. The specific marker primers can be used for quick preliminary identification of the sakura varieties including Plena Rosea, Sakuyahime and Ujou-shidare, and the method is simple, fast and accurate and is a molecular means irreplaceable by apparent characteristic identification of the sakura varieties.

The invention discloses a molecular specific marker primer and an identification method for fine varieties of camellia oleifera. Sequences of the molecular specific marker primer include upstream primers 5'-CGGACTGATTATCCTTGGCAAT-3' and downstream primers 5'-AGATCCCGCCCAAGACCTT-3'. The molecular specific marker primer and the identification method have the advantages that the fine varieties NO.21 of the camellia oleifera can be quickly identified by the molecular specific marker primer at early stages, the identification method is simple, speedy and accurate, and the molecular specific marker primer and the identification method are irreplaceable molecular means for discriminating the fine varieties of the camellia oleifera by the aid of appearance features.

The invention relates to a pair of highly specific molecular specific marker primers for Camellia oleifera varieties Changlin 3 and Changlin 21, and a method for quickly identifying the Camellia oleifera varieties Changlin 3 and Changlin 21. The primer sequences are as follows: upstream primer: 5'-AATGAATGGTGCTTTGGATT-3', downstream primer: 5'-CAAAACCACACCTTGAGTCC-3'. The molecular specific marker primer of the present invention can quickly and early identify the Camellia oleifera improved varieties Changlin Changlin 3 and No. 21, the method is simple, quick and accurate, and is an irreplaceable molecular means for distinguishing the improved Camellia oleifera varieties by appearance characteristics.

The invention relates to molecule specificity labeled primers with high specificity for improved tea-oil tree varieties No. 18 and 21 of the Changbu experimental Forestry Center, and a method for quickly identifying the improved tea-oil tree varieties No. 18 and 21 of Changbu Forestry Center. The primer sequences are as follows: the upstream primer: 5'-CTCAAGGCTTCACTAACAGCAA-3', and the downstream primer: 5'-CTGCCTCTCAATCTTGTTCACC-3'. The molecule specificity labeled primers can perform quick early-period identification on the improved tea-oil tree varieties No. 18 and 21 of the Changbu experimental Forestry Center; the method is simple, quick and accurate and is an irreplaceable molecule means for identifying the improved tea-oil tree varieties through morphologic features.

The invention relates to a pair of high-specificity molecular specific marker primers for sakura varieties including Plena Rosea, Sakuyahime and Ujou-shidare, and a method for quickly identifying the sakura varieties including Plena Rosea, Sakuyahime and Ujou-shidare. Primer sequences are as follows: an upstream primer: 5'-AGCGGCCGCCGCTAGATGAG-3'; a downstream primer: 5'-AGCGGCCGCCGAGACAAAGA-3'. The specific marker primers can be used for quick preliminary identification of the sakura varieties including Plena Rosea, Sakuyahime and Ujou-shidare, and the method is simple, fast and accurate and is a molecular means irreplaceable by apparent characteristic identification of the sakura varieties.

The invention relates to the technical fields of plant genetic engineering and molecular biology, in particular to a molecular detection method for quickly identifying true plum / apricot plum strains. The present invention provides plum blossom PmEIL protein, the sequence of which is shown in SEQ ID NO.1. PmEIL protein is involved in the response of plum blossoms to seasonal climate changes. There are obvious differences in the regulation methods of real plum and apricot plum strains by seasonal climate changes such as temperature and sunshine. Quickly and accurately identify plum blossom strains, shorten the breeding cycle, and greatly reduce the breeding workload. The invention also provides a SNP molecular marker related to the PmEIL gene and related to plum blossom stem color, which provides a basis and a molecular tool for the identification of plum blossom stem color traits and the identification of true plum / apricot plum strains.

The invention relates to a molecular specific marker primer and a detection method for Hibiscus varieties of Hibiscus syriacus 'Bricutts', Hibiscus syriacus 'Notwoodone', Hibiscus syriacus 'Sanchoyo' and Hibiscus syriacus 'Notwoodtwo', which are applied to identification of the Hibiscus varieties. The Hibiscus varieties are widely used in gardens, but are difficult to identify from the aspectof phenotypic characteristics. The molecular specific marker primer sequences of the Hibiscus varieties of Hibiscus syriacus 'Bricutts', Hibiscus syriacus 'Notwoodone', Hibiscus syriacus 'Sanchoyo'and Hibiscus syriacus 'Notwoodtwo' are: upstream primer: 5'-CTCTCTCAACTCTCATCGG-3'; downstream primer: 5'-GGAAAAAAAAAAAACA-AAAG-3'. The molecular specific marker primer can be applied to rapid earlyidentification of the Hibiscus varieties of Hibiscus syriacus 'Bricutts', Hibiscus syriacus 'Notwoodone', Hibiscus syriacus 'Sanchoyo' and Hibiscus syriacus 'Notwoodtwo'. The method is simple, fastand accurate, and is an irreplaceable molecular means for identifying Hibiscus varieties by apparent characteristics.

The invention discloses a molecular-specificity labeling primer for oil-tea good-variety longlin 18 with high specificity and a method capable of carrying out rapid identification on the oil-tea good-variety longlin 18. The sequence of the primer is as follows: the upstream primer is 5'-AGCGGCCGCGTAATAAGTCTAAGC-3', and the downstream is 5'-AGCGGCCGCGTTGGATCA-3'. The molecular-specificity labeling primer can be used for carrying out rapid and early identification on the oil-tea good-variety longlin 18, is simple, fast and accurate and is a molecular means which can not be replaced for identifying the good oil-tea variety by apparent characteristics.

The invention provides a molecule specificity marker primer of the improved variety of camellia oleifera Changlin Number 55. The sequence of the primer is that the upstream primer is 5'-CATACATACACTTCCTAAGCCAAAA-3' and the upstream primer is 5'-GTTCAAGCATTGTTCAAGCACTC-3'. The molecule specificity marker primer can be used for the early-stage identification of the improved variety of camellia oleifera Changlin Number 55. The method is simple, rapid and accurate and is a molecular method which can not be replaced by distinguishing the improved variety of camellia oleifera by appearance characteristics.

The present invention provides molecular specific marker primers for No. 4 and No.32 of an improved variety Changlin of Camellia oleifera. The sequences of the primers are: an upstream primer: 5'-CCTATGGTGCCTCTTTGTTTGATGT-3'; and downstream primer: 5'-GTGACAAGTTGAACTCTAGCACAAA-3'. By employing the molecular specific marker primers of the present invention, quick identification of the No. 4 and No.32 of the improved variety Changlin of Camellia oleifera in early stage can be achieved; the method is simple, fast and accurate, so it is a molecular method that can not be substituted by using apparent characteristics to distinguish improved varieties of Camellia oleifera.

The invention relates to a high-specificity molecular specific labeling primer for oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose' and a method capable of rapidly identifying the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'. The sequences of the specific labeling primer are as follows: the sequence of an upstream primer is 5'-AGCGGCCGCTAAGCAG-3', and the sequence of a downstream primer is 5'-AGCGGCCGCTACCTTTAC-3'. The molecular specific labeling primer can be used for carrying out rapid early phase identification on the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'; the method is simple, rapid and accurate and is an irreplaceable molecular measure for distinguishing the oriental cherry varieties from apparent characteristics.

The invention relates to a pair of high-specificity molecular specific labeled primers of an oil-tea camellia fine breed Changlin No.21 and a rapid identification method of oil-tea camellia fine breed Changlin No.21. The primers have the sequences as follows: a forward primer: 5'-TCACTCTTCAGCAGCTACCCAA-3 ', and a downstream primer: 5'-CCTTCTCTATCGCTCTCAATCTCG-3'. The molecular specific labeled primers can conduct quick early identification on oil-tea camellia fine breed Changlin No.21; the method is simple, fast and accurate, and is an irreplaceable molecular tool for discrimination of oil-tea camellia fine breed by apparent characteristics.

The invention relates to a molecule specificity labeling primer for the high-specificity tea-oil tree improved variety Chang Lin 18 and a detection method capable of rapidly detecting tea-oil tree improved variety Chang Lin 18. According to the primer sequence, the upstream primer is 5'-AGCCAAAATTGCGTCAAGTCTTCT-3', and the downstream primer is 5'-AGCAAGCAAAACCACACCCCCAA-3'. The molecule specificity labeling primer can carry out rapid early-stage detection on tea-oil tree improved variety Chang Lin 18, and the method is easy, fast and accurate and is the irreplaceable molecular method for presenting characteristics and distinguishing tea-oil tree improved varieties.

The invention relates to a pair of molecularly specific marker primers for cherry blossom varieties "Hongfeng" and "Ichihara Huwei" with high specificity, and a method capable of quickly identifying the cherry blossom varieties "Hongfeng" and "Ichihara Huwei". The primer sequences are as follows: upstream primer: 5′-AGCGGCCGCTACTAAATAGACAGC-3′, downstream primer: 5′-AGCGGCCGCTAGAAGAAGAACAAA-3′. The molecular specific marker primer of the present invention can quickly and early identify cherry blossom varieties "Hongfeng" and "Ichihara Huwei". The method is simple, fast and accurate, and is an irreplaceable molecular means for distinguishing cherry blossom varieties by appearance characteristics.