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Molecular-specific marker primers for cherry blossom cultivars "Songyue" and "Yujin"

A labeled primer and specific technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the difficulties in identification of phenotypic characteristics, application and promotion of garden plants, and difficulty in breeding new varieties and other problems, to achieve the effect of simple method

Inactive Publication Date: 2017-08-08
CHENGBANG ECO ENVIRONMENT CO LTD
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AI Technical Summary

Problems solved by technology

After long-term investigation work, we found that 20 late cherry varieties include Hongli (Cerasusserrulata'Benigasa'), Honghua (C.serrulata'Kouka'), Yiye (C.serrulata'Hisakura'), Fulushou (C. .serrulata'Contorta'), Samantabhadra (C.serrulata'Albo-rosea'), Songyue (C.serrulata'Superba'), Yujin (C.serrulata'Grandifora'), Hongfeng (Cerasus×sieboldii'Beni ‐yutaka'), Royal Clothes Yellow (C.serrulata'Gioiko'), Guanshan (C.serrulata'Kanzan'), Lantern (C.serrulata'Ojochin'), Yae Red Branch Weeping (C.spachiana'Plena Rosea') , Ichihara Huwei (C.serrulata'Albo Plena'), Saki Yeji (Cerasus×yedoensis'Sakuyahime'), Suzaku (C.serrulata'Shujaku'), Yang Guifei (C.serrulata'Mollis'), Yuqing Zhiwei ( Application of C.spachiana'Ujou‐shidare'), Chrysanthemum weeping cherry (C.serrulata'Plena-pendula'), Hirano sister back (C.serrulata'Imose'), Asahiyama cherry (C.serrulata'Asahiyama') in gardens It is extremely widespread, but the phenomenon of "same names and different objects" and "same objects and different names" of these varieties is also very serious, and it is difficult to identify from the aspect of phenotypic characteristics, which brings many difficulties to the application, promotion and selection of new varieties of garden plants. The development of stable and specific DNA fingerprints for these varieties at the molecular level is the scientific way to achieve accurate and rapid identification of cherry blossom varieties

Method used

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  • Molecular-specific marker primers for cherry blossom cultivars "Songyue" and "Yujin"
  • Molecular-specific marker primers for cherry blossom cultivars "Songyue" and "Yujin"
  • Molecular-specific marker primers for cherry blossom cultivars "Songyue" and "Yujin"

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Embodiment 1

[0038] (1) Extraction of Genomic DNA of Cherry Blossom Varieties:

[0039] Take 0.01g of the young leaves of the cherry blossom variety to be tested, add liquid nitrogen and grind them thoroughly, and use the SDS-CTAB method to extract the genomic DNA. After multiple extractions, the crude genomic DNA extract of the cherry blossom variety is obtained. The crude DNA was purified by Magabio Nucleic Acid Purification Kit (Bioer, Hangzhou, China), and detected by 1.5% agarose gel electrophoresis and DNA / RNA UV spectrophotometer (GeneQuant Pro, GE Healthcare). Integrity, Purity and Concentration. DNA samples with OD260 / OD280>1.8 were used for subsequent PCR amplification. DNA extracts were stored in a -20°C refrigerator for later use.

[0040] (2) Design specific PCR amplification primers, the sequence of the primer pair is:

[0041]The upstream primer: 5'-AGCGGCCGCACAAATTAAGAAAAA-3' and the downstream primer: 5'-AGCGGCCGCACTTTGAACGAT-3' were synthesized by Shanghai Bioengineeri...

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Abstract

The invention relates to a pair of high-specificity molecular specific marker primers for sakura varieties including Superba and Grandifora, and a method for quickly identifying the sakura varieties including Superba and Grandifora. Primer sequences are as follows: an upstream primer: 5'-AGCGGCCGCACAAATTAAGAAAAA-3'; a downstream primer: 5'-AGCGGCCGCACTTTGAACGAT-3'. The molecular specific marker primers can be used for quick preliminary identification of the sakura varieties including Superba and Grandifora, and the method is simple, quick and convenient and is a molecular means irreplaceable by apparent characteristic identification of the sakura varieties.

Description

[0001] (1) Technical field [0002] The invention relates to molecular specific marker primers of cherry blossom varieties "Songyue" and "Yujin" and a method for quickly identifying the cherry blossom varieties "Songyue" and "Yujin" by using the primers. [0003] (2) Background technology [0004] Cherry blossoms belong to the genus Cerasus of the Rosaceae (Rosaceae) and are world-renowned ornamental plants. At present, there are more than 300 known varieties of cherry blossoms, which have great application value in gardens because of their beautiful plant shape, bright colors and long flowering period. In recent years, cherry blossoms have been planted on a large scale in various parts of my country and used in parks, schools, streets, courtyards and other green spaces, becoming one of the main ornamental flowers and trees in early spring. Beijing, Wuhan, Qingdao, Shanghai and other cities hold cherry blossom festivals every year, and the cherry blossom industry has developed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 徐梁严春风李海波杨少宗魏海龙赵庆杰柳新红郭佳
Owner CHENGBANG ECO ENVIRONMENT CO LTD
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