Molecular-specific marker primers for cherry blossom cultivars "Hongfeng" and "Ichihara Huwei"

Abstract

The invention relates to a pair of molecularly specific marker primers for cherry blossom varieties "Hongfeng" and "Ichihara Huwei" with high specificity, and a method capable of quickly identifying the cherry blossom varieties "Hongfeng" and "Ichihara Huwei". The primer sequences are as follows: upstream primer: 5′-AGCGGCCGCTACTAAATAGACAGC-3′, downstream primer: 5′-AGCGGCCGCTAGAAGAAGAACAAA-3′. The molecular specific marker primer of the present invention can quickly and early identify cherry blossom varieties "Hongfeng" and "Ichihara Huwei". The method is simple, fast and accurate, and is an irreplaceable molecular means for distinguishing cherry blossom varieties by appearance characteristics.

Description

(1) Technical field [0001] The invention relates to molecular specific marker primers for cherry blossom varieties "Hongfeng" and "Ichihara Huwei", and a method for quickly identifying the cherry blossom varieties "Hongfeng" and "Ichihara Huwei" using the primers. (2) Background technology [0002] Cherry blossoms belong to the genus Cerasus of the Rosaceae (Rosaceae) and are world-renowned ornamental plants. At present, there are more than 300 known varieties of cherry blossoms, which have great application value in gardens because of their beautiful plant shape, bright colors and long flowering period. In recent years, cherry blossoms have been planted on a large scale in various parts of my country and used in parks, schools, streets, courtyards and other green spaces, becoming one of the main ornamental flowers and trees in early spring. Beijing, Wuhan, Qingdao, Shanghai and other cities hold cherry blossom festivals every year, and the cherry blossom industry has devel...

AI Technical Summary

Problems solved by technology

After long-term investigation work, we found that 20 late cherry varieties include Hongli (Cerasusserrulata'Benigasa'), Honghua (C.serrulata'Kouka'), Yiye (C.serrulata'Hisakura'), Fulushou (C. .serrulata'Contorta'), Samantabhadra (C.serrulata'Albo-rosea'), Songyue (C.serrulata'Superba'), Yujin (C.serrulata'Grandifora'), Hongfeng (Cerasus×sieboldii'Beni ‐yutaka'), Royal Clothes Yellow (C.serrulata'Gioiko'), Guanshan (C.serrulata'Kanzan'), Lantern (C.serrulata'Ojochin'), Yae Red Branch Weeping (C.spachiana'Plena Rosea') , Ichihara Huwei (C.serrulata'Albo Plena'), Saki Yeji (Cerasus×yedoensis'Sakuyahime'), Suzaku (C.serrulata'Shujaku'), Yang Guifei (C.serrulata'Mollis'), Yuqing Zhiwei ( Application of C.spachiana'Ujou‐shidare'), Chrysanthemum weeping cherry (C.serrulata'Plena-pendula'), Hirano sister back (C.serrulata'Imose'), Asahiyama cherry (C.serrulata'Asahiyama') in gardens It is extremely widespread, but the phenomenon of "same names and different objects" and "same objects and different names" of these varieties is also very serious, and it is difficult to identify from the aspect of phenotypic characteristics, which brings many difficulties to the application, promotion and selection of new varieties of garden plants. The development of stable and specific DNA fingerprints for these varieties at the molecular level is the scientific way to achieve accurate and rapid identification of cherry blossom varieties

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