Molecular-specificity labeling primer for oil-tea good-variety longlin 18 and identification method

Abstract

The invention discloses a molecular-specificity labeling primer for oil-tea good-variety longlin 18 with high specificity and a method capable of carrying out rapid identification on the oil-tea good-variety longlin 18. The sequence of the primer is as follows: the upstream primer is 5'-AGCGGCCGCGTAATAAGTCTAAGC-3', and the downstream is 5'-AGCGGCCGCGTTGGATCA-3'. The molecular-specificity labeling primer can be used for carrying out rapid and early identification on the oil-tea good-variety longlin 18, is simple, fast and accurate and is a molecular means which can not be replaced for identifying the good oil-tea variety by apparent characteristics.

Description

(1) Technical field [0001] The invention relates to a molecular-specific marker primer and an identification method of the improved Camellia oleifera variety Changlin 18. (2) Background technology [0002] Camellia oleifera is the woody oil tree species with the largest planting area and wide distribution in my country. Vigorously developing the Camellia oleifera industry is of great significance to ensuring the safety of grain and oil, increasing farmers' income, promoting the comprehensive development of mountainous areas and building a new socialist countryside. Camellia oleifera industry in my country is in the ascendant with huge development potential. At present, the total area of ​​camellia oleifera forest in my country is more than 45 million mu, but the output is very low, only about 4 kilograms of camellia oil per mu. The fundamental reason is that the vast majority of Camellia oleifera species are poor in quality or severely degraded, while a large number of nat...

AI Technical Summary

Problems solved by technology

[0004] At present, the identification of camellia oleifera varieties is mainly based on appearance characteristics, but due to the long period of identification of many morphological characters, the influence of the environment, and the increasing number of varieties, the identification of varieties is becoming more and more difficult.

Since this century, some PCR-based molecular marker technologies such as RAPD (Random Amplified Polymorphic DNA, Random Amplified Polymorphic DNA), ISSR (Inter-Simple Sequence Repeat, Simple Sequence Repeat Interval Amplified Polymorphism) and SRAP ( Sequence-related amplified polymorphism) has been successively used in the study of genetic diversity and genetic distance of Camellia oleifera germplasm resources, but there are few studies on the linkage between molecular markers and Camellia oleifera traits, and the molecular identification between Camellia oleifera varieties. Moreover, these molecular markers use universal primers, and their PCR amplification patterns are not only complex, but also poor in repeatability and specificity, so they are not suitable for variety identification. Only a stable and specific DNA fingerprint for a certain variety can be developed. Marking can really be used for accurate and rapid identification of the variety

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