Specific labeling primer for oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'

Abstract

The invention relates to a high-specificity molecular specific labeling primer for oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose' and a method capable of rapidly identifying the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'. The sequences of the specific labeling primer are as follows: the sequence of an upstream primer is 5'-AGCGGCCGCTAAGCAG-3', and the sequence of a downstream primer is 5'-AGCGGCCGCTACCTTTAC-3'. The molecular specific labeling primer can be used for carrying out rapid early phase identification on the oriental cherry varieties including Cerasus*sieboldii 'Beni-yutaka', C.serrulata 'Albo Plena' and C.serrulata 'Imose'; the method is simple, rapid and accurate and is an irreplaceable molecular measure for distinguishing the oriental cherry varieties from apparent characteristics.

Description

(1) Technical field [0001] The present invention relates to molecular specific marker primers for cherry blossom varieties "Hongfeng", "Ichihara Huwei" and "Pingye Meibei", and rapid identification of cherry blossom varieties "Hongfeng", "Ichihara Huwei" and "Pirano Meibei" by using the primers Methods. (2) Background technology [0002] Cherry blossoms belong to the genus Cerasus of the Rosaceae (Rosaceae) and are world-renowned ornamental plants. At present, there are more than 300 known varieties of cherry blossoms, which have great application value in gardens because of their beautiful plant shape, bright colors and long flowering period. In recent years, cherry blossoms have been planted on a large scale in various parts of my country and used in parks, schools, streets, courtyards and other green spaces, becoming one of the main ornamental flowers and trees in early spring. Beijing, Wuhan, Qingdao, Shanghai and other cities hold cherry blossom festivals every year, ...

AI Technical Summary

Problems solved by technology

After long-term investigation work, we found that 20 late cherry varieties include Hongli (Cerasus serrulata'Benigasa'), Honghua (C.serrulata'Kouka'), Yiye (C.serrulata'Hisakura'), Fulushou ( C.serrulata'Contorta'), Samantabhadra (C.serrulata'Albo-rosea'), Songyue (C.serrulata'Superba'), Yujin (C.serrulata'Grandifora'), Hongfeng (Cerasus×sieboldii' Beni‐yutaka'), Royal Clothes Yellow (C.serrulata'Gioiko'), Guanshan (C.serrulata'Kanzan'), Lantern (C.serrulata'Ojochin'), Eightfold Red Branch Weeping (C.spachiana'Plena Rosea' ), Ichihara Huwei (C.serrulata'Albo Plena'), Sakuyahime (Cerasus×yedoensis'Sakuyahime'), Suzaku (C.serrulata'Shujaku'), Yang Guifei (C.serrulata'Mollis'), Yuqing Zhiwei (C.spachiana'Ujou‐shidare'), Chrysanthemum weeping cherry (C.serrulata'Plena-pendula'), Hirano sister back (C.serrulata'Imose'), Asahiyama cherry (C.serrulata'Asahiyama') in the garden It is widely used, but the phenomenon of "same names and different objects" and "same objects and different names" of these varieties is also very serious, and it is difficult to identify them from the aspect of phenotypic characteristics, which brings many difficulties to the application, promotion and selection of new varieties of garden plants. Therefore, Focusing on the development of stable and specific DNA fingerprints for these varieties at the molecular level is the scientific way to achieve accurate and rapid identification of cherry blossom varieties

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