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Identification of resistance genes from wild relatives of banana and their uses in controlling panama disease

a technology of resistance genes and wild relatives, applied in the agricultural industry, can solve the problems of i>fusarium /i>wilt, the current expansion of the panama disease epidemic is particularly destructive, and the importance of crops is now severely threatened by i>fusarium /i>wil

Inactive Publication Date: 2020-12-31
EG CROP SCI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent provides methods for making plant cells resistant to a type of Fusarium oxysporum that causes disease. The methods involve introducing genetic changes into the plant's DNA to make it resistant to the fungus. These changes can be made using various techniques such as TALENs, meganucleases, zinc finger nucleases, or CRISPR-associated nucleases. The genetic changes can result in amino acid changes that make the plant cell less vulnerable to the fungus. The plant cells can be transformed with these genetic changes and then regenerated into mature plants. These mature plants can then be used in breeding programs to create resistance to Fusarium oxysporum. Overall, this patent provides a way to protect plant cells from disease and improve crop resilience to Fusarium oxysporum.

Problems solved by technology

However, this important crop is now severely threatened by Fusarium Wilt, also known as Panama Disease, caused by the fungus Fusarium oxysporum f sp.
Clearly, the current expansion of the Panama disease epidemic is particularly destructive due to the massive monoculture of susceptible Cavendish bananas.

Method used

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  • Identification of resistance genes from wild relatives of banana and their uses in controlling panama disease
  • Identification of resistance genes from wild relatives of banana and their uses in controlling panama disease
  • Identification of resistance genes from wild relatives of banana and their uses in controlling panama disease

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Experimental program
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example 1

nd Materials for Sequencing

[0378](1) Material

[0379]Fresh and lyophilized banana leaf tissues were obtained from Bioversity International (Leuven, Belgium), Inter-TROP CRB Plantes Tropicales (Guadeloupe), and the IITA Genebank (Ibadan, Nigeria), Plant Delights Nursery (Raleigh, N.C.), and The Flower Bin (Longmont, Colo.).

[0380](2) RNA

[0381]Total RNA was extracted from fresh, frozen, and lyophilized banana leaves using a modified Ishihara protocol (Ishihara et al., 2016). Approximately 100 mg of fresh or frozen banana tissue was ground to a powder using a clean, dry-ice cooled mortar and pestle that was treated with RNase Away™ (Invitrogen, Carlsbad, Calif.). Approximately 20-30 mg of lyophilized banana tissue was homogenized in a Lysing Matrix D Tube (MP Bio, Santa Ana, Calif.) without liquid. One milliliter of polyphenol lysis buffer (800 μl RLT buffer (Qiagen, Germantown, Md.), 200 μl of Fruit-mate (Takara, Mountain View, Calif.), and 10 μl of β-mercaptoethanol) was added to each s...

example 3

e Breeding of Banana

[0423]Tetraploid versions of FW-sensitive Cavendish cultivars (M. acuminata; AAAA) are available or can be developed via large pollination / breeding programs focused on creating, identifying and isolating the relatively low percentage of tetraploid progeny that are produced (e.g., Aguilar Moran, J. F., 2013, Improvement of Cavendish Banana cultivars through conventional breeding, Acta Hortic. 986:205-208; Jenny et al., In Jacome et al., editors, Mycosphaerella leaf spot diseases of banana: present status and outlook, Proceedings of the 2nd International Workshop on Mycosphaerella leaf spot diseases held in San Jose, Costa Rica, 20-23 May 2002, Session 4, pages 199-208) or by subjecting diploid AA genotypes to in vitro polyploidization (Amah et al., November 2019, Frontiers in Plant Science, Vol. 10, Article 1450, 12 pages).

[0424]Diploid versions of FW-resistant FusR1 (AA) of M. acuminata ssp. banksia can be identified or developed using methods known to those skil...

example 4

and Methods for Plant Transformation

[0428]Banana transformation systems will use sterile material of selected banana strains. A variety of tissue culture and transformation methodologies will be used to increase the likelihood of success. See, for example, the transformation protocols described in Ploetz (2015, Phytopathology 105:1512-1521), U.S. Pat. Nos. 7,534,930; 6,133,035; Sagi et al., Bio / Technology 13, 481-485, 1995; May et al., Bio / Technology 13, 485-492, 1995; Vishnevetsky et al., Transgenic Res. 20(1):61-71, 2011; Paul et al. (2011); Zhong et al., Plant Physiol. 110, 1097-1107, 1996; Dugdale et al., Journal of General Virology 79:2301-2311, 1998; Mohan and Swennen (editors), 2004, Banana improvement: cellular, molecular biology, and induced mutations, Science Publishers, Inc.; and, Remy et al., 2013, Genetically modified bananas: Past, present and future, Acta Horticulturae 974:71-80, each of which is expressly incorporated herein by reference in their entireties.

[0429]The...

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Abstract

The present disclosure provides compositions and methods for providing broad-based resistance to fungal pathogens, such as a Fusarium fungi, and plants derived therefrom.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of U.S. Provisional Patent Application No. 62 / 866,872, filed on Jun. 26, 2019, and of U.S. Provisional Patent Application No. 62 / 912,010, filed on Oct. 7, 2019, the entire contents of each of which are herein incorporated by reference.FIELD[0002]The present disclosure generally relates to the field of agricultural industry, especially production of consumer crops with pathogenic resistance. More particularly, the present disclosure relates to compositions and methods for generating plants that possess traits resistant to fungal pathogens such as the soil-born Fusarium fungi and / or that show resistance to diseases caused by said fungal pathogens.DESCRIPTION OF THE TEXT FILE SUBMITTED ELECTRONICALLY[0003]The Sequence Listing associated with this application is provided in text format in lieu of a paper copy. The contents of the text file submitted electronically herewith are incorporated herein by referen...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82
CPCC12N15/8282C07K14/415C12N15/8213
Inventor MESSIER, WALTER
Owner EG CROP SCI INC
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