Use of pirna-54265 in diagnosis, treatment, and prognostic evaluation of colorectal cancer
a colorectal cancer and prognostic evaluation technology, applied in the field of biomedical technologies, can solve the problems of poor sensitivity and specificity, difficult application, and clinically difficult to cure advanced colorectal cancer, and achieve the effects of excellent amplification efficiency and specificity of the primer designed thereof, wide application range, and easy and efficient operation
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embodiment 1
TCGA Data Analysis and Tissue Specimens of Patients Detection Indicates the Increased Expression Level of a piR-54265 in Colorectal Cancer
[0076]1. Experimental Method:
[0077]TCGA and GEO expression profile data were extracted and analyzed to obtain 20 top high-expressed piRNAs in colorectal cancer. Specific primers were designed for the top 20piRNAs, and then expression quantitation by qPCR was performed in 110 tumor tissue and paired non-tumor tissue specimens of colorectal cancer patients from Guangzhou. The expression level of the identified piR-54265 was verified in another 108 tumor tissue and paired non-tumor tissue specimens of colorectal cancer patients from Suzhou. Bioinformatics analysis of the gene copy number variation and DNA methylation modification of piRNA-54265 was performed According to the COAD data in TCGA database.
[0078]2. Experimental Results:
[0079]As shown in FIGS. 1a and 1b, only the expressions of the piRNA-54265 in the colorectal cancer tissue and the paired...
embodiment 2
High Expression Level of the piR-54265 in Cancer Tissues Associates with Poor Survival Prognosis in Colorectal Cancer Patients
[0083]1. Experimental Method:
[0084]SPSS (19.0) was used to analyze the expression of the piR-54265 in cancer tissues and the prognosis of colorectal cancer patients (such as the above-mentioned 110 colorectal cancer patients with postoperative chemotherapy from Guangzhou and 108 colorectal cancer patients with postoperative chemotherapy from Suzhou).
[0085]2. Experimental Results:
[0086]The expression level of the piR-54265 in cancer tissues was related to the survival prognosis of the colorectal cancer patients. The high-expressed piR-54265 was associated with poor prognosis of the colorectal cancer patients, having both shorter 5-year overall survival and 5-year progression-free survival. The results were consistent in the two sample sets, Guangzhou and Suzhou (as shown in FIG. 2).
embodiment 3
High Expression Level of the piR-54265 in Serum Associates with Poor Survival Prognosis of in Colorectal Cancer Patients
[0087]1. Experimental Method:
[0088]four serum specimens were randomly selected from the samples of colorectal cancer cases previously mentioned for Northern Blot to directly determine the presence or absence of piR-54265 in serum; then, RNA extraction and piR-54265 expression detection by RT-qPCR were performed on all serum specimens, and the correlation between the serum piR-54265 and the piR-54265 level in the cancer tissues was analyzed. Survival analysis was performed on the serum piR-54265 level and the prognosis of the corresponding patients. RNA was extracted from 111 serum specimens of normal people followed by piR-54265 expression detection via RT-qPCR and unpaired Student's t-test statistical analysis was performed to compare the serum piR-54265 levels of normal people and the colorectal cancer patients.
[0089]2. Experimental Results:
[0090]As shown in FIG....
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