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Mitochondrial proteins constructs and uses thereof

a technology of mitochondrial proteins and constructs, which is applied in the field of fusion protein constructs, can solve the problems of no cure for genetic mitochondrial metabolic disorders, no proven disease-modifying therapy for friedreich ataxia, and inability to penetrate the blood-brain barrier with intravenously administered enzymes,

Inactive Publication Date: 2016-03-17
YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The disclosed method can introduce a functional human mitochondrial protein into a subject's mitochondria. This introduced protein can restore at least some of the activity of a wild type human mitochondrial protein. The percentage of restoration can be at least 5%, at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or up to 100%.

Problems solved by technology

Currently, there is no cure for genetic mitochondrial metabolic disorders and treatment is mostly palliative.
However, the inability of the intravenously administered enzymes to penetrate the blood-brain barrier severely limits the application of this approach for the treatment of other metabolic disorders that involve the central nervous system (CNS) [4, 5].
Despite this vigorous international effort, there is as yet no proven disease-modifying therapy for Friedreich ataxia [22].
Repeated dosing should therefore result in accumulating amounts of cargo in the mitochondria over time.
Thus, by way of example, the long 80-aa native MTS of frataxin as well as its two-step processing can reduce its efficiency in the delivery of cargos into the matrix of the mitochondria.

Method used

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  • Mitochondrial proteins constructs and uses thereof
  • Mitochondrial proteins constructs and uses thereof
  • Mitochondrial proteins constructs and uses thereof

Examples

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Effect test

example 1

Cloning of Plasmids Encoding TAT-MTS-FRA Fusion Proteins

[0298]Expression plasmids encoding the TAT-fusion proteins were cloned and prepared by standard molecular biology tools known in the art. For a general reference see Molecular Cloning: A Laboratory manual (2001) Joseph Sambrook and David William Russell. TAT [9] having the amino acid sequence as denoted by SEQ ID NO. 27 (encoded by the nucleic acid sequence denoted by SEQ ID NO. 1) was fused (N-terminal) to the mature human frataxin protein (having the amino acid sequence as denoted by SEQ ID NO. 26 and encoded by the nucleic acid sequence as denoted by SEQ ID NO. 6).

[0299]Various fusion constructs were prepared, which differ in their mitochondrial targeting sequence present at the N terminus of human frataxin (and thus located between the TAT and mature human frataxin described above), being either the native mitochondrial targeting sequence of frataxin (referred to herein as MTSfra, having the amino acid sequence as denoted b...

example 2

Expression of the Fusion Proteins in E. coli Hosts

[0302]Expression of the fusion proteins was performed in E. coli hosts as described below and was calibrated for optimal expression conditions. As known in the art, there are several different bacterial expression systems. Successful expression of recombinant proteins is often dependent on the strain of the bacteria expression system used. Thus, for each fusion protein prepared as described above, four different E. coli bacterial stains were tested: BL21-CodonPlus, BL21, Rosetta and HMS (Invitrogen, USA) and the host for expression was thereby selected. The conditions for expression were also calibrated for each of the TAT-fusion proteins, by changing several parameters, including the concentration of the inducer Isopropyl β-D-1-thiogalactopyranoside (IPTG) and length of induction growth conditions (i.e. temperature, addition of chemicals, etc.)

[0303]Upon expression, bacterial cells were disrupted and cellular sub-fractions were prep...

example 3

Purification of the TA T-MTS-FRA Fusion Proteins

[0307]The soluble fractions of the expressed TAT-MTS-FRA fusion proteins were loaded onto a nickel-chelating column to affinity-purify these proteins, as detailed above. Calibration experiments were performed for each of the fusion proteins, including specific conditions for binding of the fusion protein onto the affinity column, its elution and removal of the imidazole from the final protein preparations. One typical purification run of each of the fusion proteins is demonstrated in FIG. 4 (for His-TAT-MTSfra-FRA and His-TAT-MTSorf-FRA) and in FIG. 5 (for His-TAT-MTSlad-FRA and His-TAT-MTScs-FRA).

[0308]For example, FIG. 4A shows an image of an affinity chromatography purification profile obtained for the fusion protein TAT-MTSfra-FRA. As can be seen in FIG. 4B, which is an image of SDS-PAGE analysis of the purification steps of this protein construct, the protein fraction shown in FIG. 4B, lane 7, represents the fusion protein TAT-MTS...

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Abstract

Disclosed are novel fusion protein constructs comprising a functional mitochondrial protein, that can enter mitochondria within intact cells. Further disclosed are methods of treating mitochondrial disorders by the disclosed fusion proteins and compositions therefor.

Description

TECHNOLOGICAL FIELD[0001]Disclosed are novel fusion protein constructs comprising a functional mitochondrial protein. Further disclosed are methods of treating mitochondrial disorders by the disclosed fusion proteins and compositions therefor.PRIOR ART[0002]References considered to be relevant as background to the presently disclosed subject matter are listed below:[0003][1] Chinnery P F, Schon E A (2003) Mitochondria. J Neurol Neurosurg Psychiatry 74: 1188-1199.[0004][2] DiMauro S, Schon E A (2003) Mitochondrial respiratory-chain diseases. N Engl J Med 348: 2656-2668.[0005][3] Brautigam C A, Chuang J L, Tomchick D R, Machius M, Chuang D T (2005) Crystal structure of human dihydrolipoamide dehydrogenase: NAD+ / NADH binding and the structural basis of disease-causing mutations. J Mol Biol 350: 543-552.[0006][4] Brady R O, Schiffmann R (2004) Enzyme-replacement therapy for metabolic storage disorders. Lancet Neurol 3: 752-756.[0007][5] Wang D, Bonten E J, Yogalingam G, Mann L, d'Azzo A...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/435A61K9/00C07K14/005C12N7/00C12N9/02C12N9/10
CPCC07K14/435A61K9/0019C07K14/005C12N7/00C12N9/0051C07K2319/07A61K38/00C12N2740/16033C12Y203/03001C12Y108/01004C07K2319/10C12N9/1025C07K14/47C12N9/1018C07K2319/21Y10S530/826Y10S530/827
Inventor LORBERBOUM-GALSKI, HAYAGREIF, HAGAR
Owner YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD
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