Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Production of polyhydroxyalkanoates with a defined composition from an unrelated carbon source

a technology of monomeric composition and polyhydroxyalkanoate, which is applied in the direction of lyase, ligase, enzymology, etc., can solve the problems of low-cost sources of such monomers or monomer precursors, the inability to produce mcl-pha at high yields from an unrelated carbon source, and the inability to meet the requirements of specific commercial applications

Inactive Publication Date: 2014-03-13
WISCONSIN ALUMNI RES FOUND
View PDF10 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention describes a method for producing a specific type of polyhydroxydodecanoate (mcl-PHA) from glucose in a microorganism like E. coli. This involves combining fatty acid biosynthesis, β-oxidation, and a PHA polymerase. By expressing certain enzymes and overexpressing others, the method allows for high yield production of mcl-PHA from glucose under aerobic conditions. This is the highest reported production of mcl-PHA from an unrelated carbon source.

Problems solved by technology

Such PHA polymers with random monomeric sequences are often not desirable for specific commercial applications.
However, this strategy requires an external source of each monomer or monomer precursor (e.g., fatty acids), and low-cost sources of such monomers or monomer precursors are not currently available.
However, production of mcl-PHA at high yields from an unrelated carbon source has not been achieved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production of polyhydroxyalkanoates with a defined composition from an unrelated carbon source
  • Production of polyhydroxyalkanoates with a defined composition from an unrelated carbon source
  • Production of polyhydroxyalkanoates with a defined composition from an unrelated carbon source

Examples

Experimental program
Comparison scheme
Effect test

examples

Summary

[0138]The following Examples present a rational approach for producing mcl-PHA homopolymer from an unrelated carbon source (i.e., glucose) in E. coli. A characterization of a panel of mutant E. coli strains to determine the impact of β-oxidation enzymes on fatty acid consumption and mcl-PHA synthesis is presented. A characterization of two PHA synthases (PhaC) and four enoyl-CoA hydratases (PhaJ) for producing mcl-PHA in E. coli, thereby identifying a suitable combination for making mcl-PHA, is also presented. An examination of the impact of different modes of regulating acyl-CoA synthetases on PHA titer is shown. Finally, engineering of a strain of E. coli to produce mcl-PHA with a composition matching the product profile of the expressed thioesterase is shown. The strategy involves constructing a strain of E. coli in which key genes in fatty acid β-oxidation are deleted and BTE, phaJ3 and phaC2 from Pseudomonas aeruginosa PAO1, and PP—0763 from P. putida KT2440 are overexpr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
optical densityaaaaaaaaaa
temperatureaaaaaaaaaa
v/vaaaaaaaaaa
Login to View More

Abstract

Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority under 35 USC §119(e) to U.S. Provisional Patent Application 61 / 699,044 filed Sep. 10, 2012, the entirety of which is incorporated herein by reference.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH[0002]This invention was made with government support under DE-FC02-07ER64494 awarded by the US Department of Energy. The government has certain rights in the invention.FIELD OF THE INVENTION[0003]The present invention is directed to cells and methods for producing polyhydroxyalkanoates having a defined monomeric composition at a high yield from an unrelated carbon source.BACKGROUND[0004]Polyhydroxyalkanoates (PHA) are a class of microbially synthesized polyesters that are produced in large quantities as a form of carbon and energy storage. Natural PHA possesses structural properties that make it attractive as a renewable plastic for select applications. However, most naturally produced PHA contains random monom...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12P7/62
CPCC12P7/62C12Y402/01017C12N9/0006C12N9/1029C12N9/16C12Y101/01035C12N9/88C12N9/93C12P7/625C12N1/20
Inventor PFLEGER, BRIAN FREDERICKAGNEW, DANIEL E.
Owner WISCONSIN ALUMNI RES FOUND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com