Modified Mareks Disease Virus, and Vaccines Made Therefrom
a vaccine and virus technology, applied in the field of virus vaccines, can solve the problems of sporadic losses, sb-1 strain is poorly protective against highly virulent mdv strains, and the poultry industry continues to experience losses due to md, so as to reduce economic losses and improve the viability and productivity of chickens
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[0044]The present invention provides Recombinant Marek's disease virus (MDV), into which has been inserted via homologous recombination, a long terminal repeat (LTR) derived from a reticuloendotheliosis virus (REV). These recombinants are effective to elicit an immune response in an avian to Marek's disease virus without causing a significant degree of pathogenicity in the avian. As used herein, “without causing a significant degree of pathogenicity” is defined as no gross MD-specific lesions being observable with the naked eye in the inoculated / challenged avian, even in highly susceptible avians. In particular embodiments, the avians are chickens.
[0045]CVRM-2 was produced by authors of Reddy et al., as described therein, and as summarized in FIGS. 1 and 2 of this disclosure. Upon receipt of the CVRM2 sample, instant Applicants performed careful PCR-based analysis to confirm the identity / integrity of the virus isolate (FIG. 3 presents the agarose gel resolution of the amplified prod...
example 1
Efficacy of Marek's Disease Virus (MDV), SR-1 Strains, CVRM-2 RN1250 and RMI CN32399, and Rispens CN32553
[0071]A critical aspect of the instant invention is that after having completed the study outlined in this Example, inventors later determined the CVRM-2 MDV was not a clonal recombinant MDV, but in fact was a mixed population of RN1250 recombinant MDV and parental Rispens MDV. Thus, now that inventors have produced a stable, clonal RN1250 MDV, which virus' strong efficacy as a vaccine is demonstrated in later Examples, a skilled person will not be surprised by the broad (and unacceptable) range of protection (37-86%) apparently provided by the CVRM-2 MDV in this preliminary study.
[0072]Objective.
[0073]To evaluate and compare the efficacy of three experimental MDV SR-1 and Rispens strains to a commercial vaccine product (Rismavac®-Intervet, Inc.) in SPF Chickens using an early challenge with MDV T. King.
[0074]Materials / Methods.
[0075]One hundred fifty one-day-old SPF chicks (SPAFA...
example 2
Efficacy of MDV, SR-1, RN1250 Vaccine in Commercial Broilers Using a Shedder Challenge Model
[0078]Materials & Methods:
[0079]Seventy-six (76) one-day-old commercial broilers obtained from Harrison poultry flock 1-1 were randomized into three different colony houses and four different groups as follows: Group 1: 13 birds; Group 2: 13 birds; Group 3: 25 birds; and Group 4: 25 birds (one house was split into two sides in order to create pen one and pen two). After randomizing, the birds were banded in the nape of the neck for identification and then were inoculated with 0.2 ml per bird intraperitoneally (IP) with vvMDV T. King challenge, diluted 1:500. After challenging, the birds were placed into their respective colony house with 25-26 birds per house. These birds remained in the colony houses for 14 days prior to the placement of MDV vaccinated and non-vaccinated, contact control birds. Fourteen days after placement of the shedder birds, 304 one-day-old commercial broilers were rando...
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