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Arrays and methods for guided cell patterning

Inactive Publication Date: 2010-02-18
UNIV OF WASHINGTON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0021](b) individually addressing one or more of the treated ce

Problems solved by technology

Despite the encouraging advances made with micropatterning of living cells on substrates, patterning single cells on a microarray and retaining their viability for a prolonged period of time remain as a challenge.
However, adhesion sites of such small areas tend to suppress cell spread and thus are prone to causing cell death.

Method used

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  • Arrays and methods for guided cell patterning
  • Arrays and methods for guided cell patterning
  • Arrays and methods for guided cell patterning

Examples

Experimental program
Comparison scheme
Effect test

example 1

Representative Cell-Patterning Arrays With Cell-Adhesion Peptides Attached to Cell-Adhesion Sites

[0155]In this example, the preparation of a dry oxidized, native oxide depleted silicon surface useful in making representative cell-patterning array of the invention is described.

[0156]Substrate Preparation. Four-inch p-type silicon substrates with (100) orientation were cleaned with piranha (hydrogen peroxide / sulfuric acid 2:5 v / v) at 120° C. for 10 minutes, dipped in HF, and rinsed with DI water thoroughly. A 1.1 μm layer of positive photoresist was then coated on the surface, and an array of 20 μm×20 μm gold squares (electrodes) spaced 60 μm apart was patterned on silicon oxide substrates by conventional microfabrication as follows. A 10 nm layer of titanium (Ti) was then deposited onto the photoresist-developed substrates at a deposition rate of 0.3 Å / s. A gold film of 100 nm in thickness was subsequently deposited onto the Ti at a deposition rate of 5 Å / s. The photoresist was disso...

example 2

Detection of Bacterial Infection using a Representative Cell-Patterning Array

[0162]In this example, a method for using a representative array of the invention for detecting bacterial infection is described.

[0163]Substrate Preparation. Four inch p-type silicon substrates of (100) orientation were cleaned with piranha (hydrogen peroxide / sulfuric acid 2:5, v / v) at 120° C. for 10 minutes, dipped in HF, and thoroughly rinsed with DI water. A layer (1.1 μm) of positive photoresist was then coated on the surface, and patterns were formed on the substrate upon exposure to ultraviolet light through a mask with square patterns of three different sizes (25, 100, and 400 μm2). A titanium (Ti) layer (10 nm) was then deposited on the photoresist-developed substrates at a deposition rate of 0.3 Å / s. A gold film of 100 nm thickness was subsequently deposited on the Ti at a deposition rate of 5 Å / s. The photoresist was dissolved in acetone and the remaining metal film was lifted off. After lift off,...

example 3

Comparison of Cell-Patterning Arrays Based on Native Silicon Surface, Wet Oxide Surface, and Dry Oxide Surfaces

[0170]In this example, the preparations of cell-patterning arrays based on native silicon surface, wet oxidized native oxide depleted silicon surface, and dry oxidized native oxide depleted silicon surface are described. The cellular viability and stability of these arrays are compared.

[0171]Substrate Preparation. Four inch p-type silicon substrates of (100) orientation were cleaned with piranha (hydrogen peroxide / sulfuric acid 2:5 v / v) at 120° C. for 10 minutes, dipped in HF, and rinsed with DI water thoroughly. A layer of positive photoresist (1.1 μm) was then coated on the surface, and an array of squares (20 μm×20 μm) was patterned on the substrate upon exposure to UV light through a mask. A thin layer of titanium (Ti) of 10 nm in thickness was then deposited onto the photoresist-developed substrate at a deposition rate of 0.3 Å / s. Gold films of 100 nm in thickness were...

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Abstract

Guided cell patterning arrays for single cell patterning, methods for making the arrays, and methods for using the arrays.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application is a continuation of International Application No. PCT / US2008 / 050307, filed Jan.4, 2008, which claims the benefit of Provisional Application No. 60 / 883,480, filed Jan. 4, 2007. Each application is incorporated herein by reference in its entirety.STATEMENT OF GOVERNMENT LICENSE RIGHTS[0002]This invention was made with Government support under Contract No. 5R01 GM075095, awarded by the National Institutes of Health and under Contract No. EEC9529161, awarded by the National Science Foundation. The Government has certain rights in the invention.BACKGROUND OF THE INVENTION[0003]The ability to position and probe a single cell is of great interest in fundamental cell biology, cell-based biosensor technologies, medical diagnostics, and tissue engineering. Because critical cell-to-cell differences are lost in average bulk cell measurements, the single cell analysis with its ability to reveal the response of each individual cell und...

Claims

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Application Information

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IPC IPC(8): C40B30/04C40B40/02C40B50/06
CPCG01N33/5005G01N33/5008C40B20/02C40B40/02C40B60/04H01L21/02164
Inventor ZHANG MIQINVEISEH MANDANA
Owner UNIV OF WASHINGTON
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