Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats
a technology of biological macromolecules and electrophoresis, which is applied in the direction of fluid pressure measurement, liquid/fluent solid measurement, peptide measurement, etc., can solve the problems of difficult two-dimensional separation of complex mixtures using polyacrylamide electrophoresis, and achieves easy two-dimensional gel electrophoresis, reduces streaking, and reduces the time without electric power
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Step 1: Mount Dehydrated Strips on Manifold
[0057]FIG. 5A shows partially dehydrated pre-cast polyacrylamide pH gradient gel strips (1), bound to a perforated plastic backing (2) at 9.0 millimeter spacing, are pressed into molded channels (8) on one side of an elastomeric manifold (6) so that each strip is sealed with a laminae space above the gel. Each laminar space is accessible for filling through two normally closed diagonal ports, top and bottom on the opposite side of the elastomeric manifold from the strips.
Step 2: Load Samples
[0058]FIG. 5B shows how the elastomeric manifold (6) is mounted at an angle on the diagonal support stand (7). Samples containing proteins to be focused are loaded through the bottom ports (10), pushing open the collapsible channels (11) and filling the laminae space above each strip. Sample loading is most easily accomplished using an 8-place pipettor. Sets of disposable pipette tips are first inserted in the top ports. The tips hold the top port (10) o...
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