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Method for increasing stress tolerance in plants

a technology for stress tolerance and plants, applied in the field of methods and compositions for can solve the problems of reducing stress inducibility in plants, and achieve the effect of increasing stress tolerance in plants

Inactive Publication Date: 2008-07-31
THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides methods and compositions for increasing stress tolerance in plants by overexpressing a molybdenum cofactor sulfurase in the plant. This enzyme plays a crucial role in the biosynthesis of the plant hormone ABA, which is involved in the regulation of stress responses. The invention also provides isolated and purified molybdenum cofactor sulfurase and an enzyme with similar activity. The invention also involves the use of a reporter gene approach to genetically dissect ABA and stress signal transduction in plants. The invention can be used to produce plants or plant cells with increased stress resistance, and can also be used to understand the regulation of stress gene expression and stress tolerance in plants.

Problems solved by technology

Secondly, the expression of many stress-responsive genes is induced by exogenous ABA, and their stress-inducibility is decreased in mutant plants defective in ABA biosynthesis or responsiveness.

Method used

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  • Method for increasing stress tolerance in plants
  • Method for increasing stress tolerance in plants
  • Method for increasing stress tolerance in plants

Examples

Experimental program
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example 1

Characterization of the LOS5 / ABA3 Locus

Isolation of los5 Mutants

[0142]Arabidopsis thaliana (C24 ecotype) expressing the RD29A-L UC transgene (referred to as the wild type) were obtained by Agrobacterium-mediated transformation (Ishitani et al., 1997). Seeds of wild type RD29A-LUC were mutagenized by ethyl methanesulfonate (Ishitani et al., 1997). M2 were planted on 0.6% agar plates containing full strength Murashige and Skoog salt base (MS salt base; JRH Biosceicnes, Lenex, Kans.) and were germinated and grown at 22±2° C. under continuous white light. One-week-old seedlings were screened for mutants with altered RD29A-LUC (i.e. luminescence) expression in response to low temperature, ABA, or osmotic stress using a thermoelectrically cooled CCD camera. For luminescence imaging, plants were sprayed with 1 mM luciferin in 0.01% Triton X-100 and then kept in the dark for 5 min before imaging. All images were acquired with a 5 min exposure time. The luminescence intensity of each seed...

example 2

Overexpression of the LOS5 Gene Increases Plant Tolerance to Drought Stress

[0151] Since loss of function mutations in the LOS5 gene resulted in de-regulated expression of stress-responsive genes and increased sensitivity to drought and salt stress, it was hypothesized that increased expression of LOS5 might affect gene expression pattern under stress and increase plant drought tolerance.

[0152] To test this possibility, LOS5 full length cDNA was transcriptionally fused to the CaMV 35S promoter and a transcriptional enhancer. The insert was cloned into the binary vector pCAMBIA1200. The resulting plasmid was transferred into Arabidopsis ecotypes Columbia and C24 (containing the RD29A-LUC reporter gene), respectively. Around 70 transformants were selected in each ecotype background for further tests. To examine the impact of overexpressing LOS5 gene on stress gene regulation and plant water relations, seedlings of transgenic plants were assayed for 1) their expression of the RD29A-LU...

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Abstract

The present invention provides methods and compositions for improving the drought resistance of plants. More specifically, the present invention utilizes overexpression of a molybdenum cofactor sulfurase in plants and plant cells.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims benefit to U.S. Provisional Application Ser. No. 60 / 317,724, filed on Sep. 6, 2001, incorporated herein by reference in its entirety.STATEMENT OF GOVERNMENTAL SPONSORSHIP [0002] The invention described herein was supported by NSF grants IBN-9808398 and DBI-9813360. The government may have certain rights in this invention.BACKGROUND OF THE INVENTION [0003] 1. Field of the Invention [0004] The present invention relates to methods and compositions for increasing stress tolerance in plants. More specifically, the present invention provides methods and compositions for improving drought resistance, salt tolerance, and resistance to freezing. [0005] 2. Background of the Invention [0006] Plants respond to environmental challenges in part by altering their gene expression profile that ultimately leads to various adaptive responses at cellular and whole plant levels (Bray, 1993; Thomashow, 1999; Hasegawa et al., 2000; Zhu...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01H1/00C12N15/82A01FA01F1/00A01H5/00A01H5/10C12N5/04C12N5/10C12N9/00C12N9/02C12N9/10C12N15/09C12N15/29C12N15/52C12N15/90
CPCC12N15/8273C12N9/13A01H1/00A01F1/00
Inventor ZHU, JIAN-KANGXIONG, LIMING
Owner THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA
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