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Nanofiber construct and method of preparing thereof

a nanofiber and construct technology, applied in the field of nanofiber constructs and methods of preparing them, can solve the problems of inability to use implants, decrease in the tensile property of these membranes, etc., and achieve the effects of enhancing the hydrophilicity of composite nanofiber constructs, enhancing cell attachment, and enhancing the osteoconductive property of nanofibers

Inactive Publication Date: 2006-07-13
NAT UNIV OF SINGAPORE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0021] Accordingly, the present invention also provides the fabrication method and / or surface modification of composite nanofiber constructs. The invention enhances cell attachment and proliferation and by creating a suitable construct the mechanical strength of the construct can be appropriately tailored to meet the intended use.
[0035] Therefore, a fabrication method and / or surface modification of polymer composite nanofiber construct(s) is provided. The method comprises the preparation of polymer solution comprising filler particles such as calcium salt particles, the principle of electrospinning method and preferably air-plasma treatment to enhance the hydrophilicity of composite nanofiber constructs. Additionally, nanofibers constructs are formed by electrospinning two or more types of nanofibers either simultaneously or sequentially in layers. In the composite nanofiber construct, the diameter and composition of one type of nanofiber may be adjusted for cell attachment and growth while the diameter and composition of the other may be adjusted for mechanical strength. In another type of composite nanofiber construct, the diameter and composition of each type of nanofiber is selected for cell attachment and proliferation for specific desired cell types. Other aspects, features and advantages of the invention will become apparent to those of ordinary skill in the art upon review of the description of specific embodiments of the invention. Calcium salt nanoparticles are added to the solvent mixture to result in composite nanofibers to enhance cell attachment, in particular osteoblast attachment. Such composite nanofibers are suitable for use as bone graft substitutes. The addition of calcium salt nanoparticles enhances the osteoconductive property of the nanofibers. Furthermore, human osteoblasts prefer a calcium rich environment. Composite nanofibers embedded with calcium phosphate can enhance cell attachment and growth. Besides nanometer scale fiber constructs, functionalization (surface modification) of nanofibers is of paramount importance to promote better cell fiber interaction.

Problems solved by technology

However, they also found that the tensile property of these membranes decreased with the increase of amounts of calcium carbonate particles.
Accordingly, these membranes were not suitable for implant uses as they are mechanically not stable.

Method used

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  • Nanofiber construct and method of preparing thereof
  • Nanofiber construct and method of preparing thereof
  • Nanofiber construct and method of preparing thereof

Examples

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example 1

[0116] In the present invention, the composite nanofiber constructs were prepared by ε-polycaprolactone (PCL) nanofibers and a composite of PCL and calcium carbonate nanoparticles (CaCO3) nanofibers with a particular weight ratio, i.e., PCL:CaCO3=25:75 wt %). The materials used were PCL pellet (Mn=80,000) purchased from Sigma-Aldrich Singapore Pte. Ltd., and CaCO3 nanoparticles (average particle size=40 nm: cubic type) supplied from NanoMaterials Technology Pte. Ltd. Singapore. For PCL nanofibers, the PCL pellet was first dissolved in a mixture of solvent comprising 75 wt % chloroform and 25 wt % methanol. The concentration of PCL solution was 7.5 wt % to ensure fine fiber morphology in the resulting nanofibers. For PCL / CaCO3 composite nanofibers, CaCO3 nanoparticles were first dissolved in a mixture of solvent and subsequently, the PCL pellet was dissolved. The concentration of PCL in the resulting mixture was 5 wt %. The outlet was a needle with 0.21 mm inner diameter. The feed ra...

example 2

[0118] Please note that with reference to this example, GBR membrane (A) refers to nanofiber (A) and GBR membrane (B) refers to nanofiber (B).

2.1 Fabrication of Composite Nanofibrous Construct

2.1.1 Electrospinning

[0119] In this example, composite nanofibrous constructs were designed by epsilon-polycaprolactone (PCL) nanofibers and PCL / CaCO3 composite nanofibers with two different weight ratios (i.e. PCL:CaCO3=75:25 wt % and 25:75 wt %). The materials used were PCL pellet (Mn=80,000) purchased from Sigma-Aldrich Singapore Pte. Ltd., and CaCO3 nanoparticles (average particle size of 40 nm, cubic type) supplied by NanoMaterials Technology Pte. Ltd. Singapore.

[0120] For PCL nanofibers, the PCL pellet was first dissolved in a mixture of 75 wt % chloroform and 25 wt % methanol. In order to obtain fine fiber morphology, the concentration of PCL solution was varied in the range from 3 wt % to 7.5 wt %.

[0121] For PCL / CaCO3 composite nanofibers, CaCO3 nanoparticles were first dissolved...

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Abstract

The present invention provides a composite nanofiber construct comprising: at least a first nanofiber comprising at least a polymer and at least a calcium salt nanoparticle, wherein the ratio of polymer to calcium salt nanoparticle is between the range of 99:1 and 10:90 weight percent; and at least a second nanofiber comprising at least a polymer and at least a calcium salt nanoparticle, wherein the ratio of polymer to calcium salt nanoparticle is between the range of 100:0 and 70:30 weight percent. The present invention also provides a method of preparing the composite nanofiber construct.

Description

CROSS-REFERENCE TO RELATED APPLICATION [0001] This application claims the benefit of U.S. Provisional Application No. 60 / 636,356, filed on Dec. 15, 2004, the entirety of the contents of which are hereby incorporated by reference herein.FIELD OF THE INVENTION [0002] The present invention relates to a nanofiber construct and method of preparing the same. BACKGROUND OF THE INVENTION [0003] Different types of polymer fibers with nanometer scale diameter have been recently prepared by electrospinning method. As compared to the conventional polymer fibers with micrometer scale, nanofibers have a high surface area-to-volume ratio. Hence, electrospun nanofibers appear to have better potential in several bioengineering applications, such as tissue regeneration, biosensors, recognition and filtration of viruses and drug molecules. [0004] The interaction between nanofiber scaffolds and proliferation cells, like human osteoblasts, smooth muscle cells, mesenchymal stem cells and chondrocytes hav...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): D02G3/00
CPCA61L27/18A61L27/46A61L2400/12A61L2400/18Y10T428/2929D01F6/625D01D5/0007D01F1/10C08L67/04
Inventor FUJIHARA, KAZUTOSHIKOTAKI, MASAYARAMAKRISHNA, SEERAMCHAN, KWAN-HO C.
Owner NAT UNIV OF SINGAPORE
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