Coating process for microfluidic sample arrays

Abstract

A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. A first one of the dispenser array and the microfluidic array is movable in relation to the frame, and the other of the first one of the dispenser array and the microfluidic array is fixed relative to the frame. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application is a regular U.S. application, and claims priority from U.S. Application Ser. No. 60 / 599,217 filed Aug. 4, 2005 entitled Differential Microfluid Coating process; and U.S. Application Ser. No. 60 / 608,231 filed Sep. 9, 2004 entitled “Calibration of Dispensers Relative to a Microfluidic Array,” each of which is incorporated by reference herein.TECHNICAL FIELD [0002] The present invention relates to techniques for transferring small volumes of liquid, and more specifically to calibration of dispensers relative to nanoliter sample volumes in an array. The present invention also relates to processes and the devices for spatially selective chemical modification or coating of the surfaces of a substrate or through-hole array plate, such as may be used in microfluidic or nanofluidic storage and analysis systems or other applications. BACKGROUND ART [0003] Various systems are known for performing a large number of chemical and bi...

AI Technical Summary

Benefits of technology

The patent describes a method for registering a dispenser array to a microfluidic array in relation to a frame. The method involves identifying a fiducial reference on the microfluidic array and a fixed position on the dispenser array. The relative position of the dispenser array is determined, and quantities of vector displacement from the fixed position to the fiducial reference are determined. These quantities are used to guide positioning of the dispenser array relative to the microfluidic array. The method may involve using a first camera and a second camera to determine the position of the dispenser array and the microfluidic array. The patent also describes a system for identifying the position of the dispenser array and the microfluidic array in relation to a world coordinate system.

Problems solved by technology

However, nanoliter chips require complex time-consuming preparation and processing.

Transferring large collections of fluid samples such as libraries of small molecule drug candidates, cells, probe molecules (e.g., oligomers), and / or tissue samples stored in-older style 96- or 384-well plates into more efficient high density arrays of nanoliter receptacles can be difficult.

Any misalignment of the pins to the through-holes on the chip will result in a failure to properly load the microfluidic array with sample.

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