Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extract of cercis chinensis having anti-oxidant activity and anti-aging activity, and cosmetical composition containing the extract for anti-oxidation, skin-aging protection and wrinkle improvement

a technology of applied in the field of plant extracts with antioxidant activity and anti-aging activity, and cosmetic compositions containing the extract for anti-oxidation, skin aging protection and wrinkle improvement, can solve the problems of low radical scavenging activity, and low individual oriented study on aging. , to achieve the effect of increasing the dpph scavenging activity, low radical scavenging activity, and high radical

Inactive Publication Date: 2006-04-13
HANSAENG COSMETIC +1
View PDF1 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"This patent describes a new extract from a plant called Cercis chinensis that has antioxidant, anti-skin aging, and skin elasticity-protecting properties. The extract can be made using water or alcohol as an extractant. The patent also describes a cosmetic composition and a pharmaceutical composition that contain this extract as an effective ingredient. The patent also provides a method for preparing the extract."

Problems solved by technology

Even in an individual, aging shows different aspects in each organ, so that individual oriented study on aging has a limitation.
After all the efforts made by numbers of scientists to explain aging completely, the exact mechanism of aging has not been disclosed yet because of its various aspects and complexity.
But, the price is high and the effect thereof is doubtful owing to the instability of the chemical mixture.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extract of cercis chinensis having anti-oxidant activity and anti-aging activity, and cosmetical composition containing the extract for anti-oxidation, skin-aging protection and wrinkle improvement
  • Extract of cercis chinensis having anti-oxidant activity and anti-aging activity, and cosmetical composition containing the extract for anti-oxidation, skin-aging protection and wrinkle improvement
  • Extract of cercis chinensis having anti-oxidant activity and anti-aging activity, and cosmetical composition containing the extract for anti-oxidation, skin-aging protection and wrinkle improvement

Examples

Experimental program
Comparison scheme
Effect test

example 7

Activity to Protect Cells from Oxidative Stress Caused by UV Irradiation

Cell Protecting Effect from UV Irradiation (in Vitro)

[0115] UV irradiation induces DNA damage and DNA-protein connection by increasing active oxygen in cells. When HEK-N / F cells were irradiated by 35 mJ / cm2 of UVB, DNA chains in nucleus were cut. After uniting the broken chains to ethidium homodimer (Et2), a DNA chain intercalating fluorescent dye, extent of fluorescence was measured to quantify the amount of DNA damage. Particularly, HEK-N / F cells were cultured on serum-free KGM medium (Clonetics), which were inoculated into wells of a 24-well plate by 0.5-4×104 cells / 2 cm2. Then, the cells were cultured for 18 hours, after which a sample was treated thereto for 2 hours. After being washed with PBS, the cells were irradiated by UV using a UV transilluminator (Spectronics UV transilluminator EBF-260, the maximal wavelength, 312 nm; a half-peak intensity range, 297-328 nm). The cells exposed on UV were further...

example 8

Prolongation of Life Span of a Cell

[0122] HEK-N / F cells were obtained from foreskin of a 5 newborn baby after its fibroblasts, another major component of skin, were treated with uridine bromide. Then, the cells were cultured in DMEM medium supplemented with 10% FBS. HEK-N / F cells were diluted consecutively at the ratio of 1:4 during the culture. Before being treated with samples, the cells were grown up to 3 PDL (population doubling level). During the culture, each sample was administered by 3 g / ml. Medium was replaced every three days by fresh medium supplemented with a culture solution containing the equal concentration of the sample. After culturing, an extract of Cercis chinensis of the present invention, a compound represented by (piceatannol) and a compound represented by (myricetin) were treated, followed by investigation of the cell division.

[0123] As a result, an extract of Cercis chinensis and its active ingredients were all confirmed to have an effect of prolonging li...

example 9

Prolongation of Life Span of a Cell and Extension of a Telomere

[0124] In the above Example 8, an extract of Cercis chinensis and active ingredients separated from the same were confirmed to have an effect of prolongation of life span of a cell. Thus, in this example, relation between the effect of prolongation of life span and the length of a telomere was investigated. Particularly, genomic DNA was extracted from each cell of different ages by using a nucleic acid extraction kit (IsoQuick Nucleic Acid Extraction kit, ORCA Research Inc.), which was stored at 4° C. after being dissolved in Tris-EDTA solution (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). 2 μl of 10×H buffer solution (TaKaRa), 2 μl of the extracted genomic DNA solution and distilled water were all mixed in a 1.5 ml tube, making final volume 19 μl. Then, 1 M of restriction enzyme Hinf I (6 U / l, TaKaRa) was added thereto. The mixture was reacted at 37° C. for 3-4 hours, followed by electrophoresis. For the electrophoresis, agaros...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to extract of Cercis chinensis having anti-oxidant activity and antiaging activity containing compound of chemical formula 1 to chemical formula 20, and cosmetical composition for anti-oxidation, skin-aging protection and wrinkle improvement containing the extract as effective ingredient. The extract of present invention having protective effect on oxidative damage and skin damage, and inhibitory effect on age-dependent telomere shortening, so it can effectively used as skin-aging protection comestic.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a plant extract having an anti-aging activity and a cosmetic composition containing the same as an effective ingredient. More particularly, the present invention relates to an extract of Cercis chinensis having an anti-oxidant activity and an anti-aging activity, and a cosmetic composition for anti-oxidation, skin-aging protection and wrinkle improvement containing the extract as an effective ingredient. BACKGROUND [0002] Aging means all the physiological changes of a body occurring by the lapse of time and the aging aspects and speed differ in each individual case and are affected by numbers of reasons. Even in an individual, aging shows different aspects in each organ, so that individual oriented study on aging has a limitation. More particularly, the functions of each organ and tissue are changed by aging, which is caused by the change of cell function. For example, the damage of nerve cells of brain causes the decrea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/48A61K8/97A61Q19/08
CPCA61K8/97A61K36/48A61K45/06A61K2800/522A61Q19/00A61Q19/08A61K8/9711A61K8/9789A61P17/00A61P17/16A61P39/06
Inventor NA, MIN KYUNYOO, JAE-KUKLEE, CHAN BOGKIM, JIN PYOLIM, GON HYEOKMIN, DONG ILJEON, YOUNG MIN
Owner HANSAENG COSMETIC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com