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Microfluidic chip or biomolecule crystallization

a microfluidic chip and biomolecule technology, applied in the field of microfluidic systems and methods for crystallizing biomolecules, can solve the problems that the prior crystallization devices available for high throughput screening of crystallization conditions cannot address at least some of the features listed below, and achieve the effect of promoting crystal growth

Inactive Publication Date: 2005-05-26
GILBERT JOHN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Enables high-throughput protein crystallization with reduced sample and reagent consumption, allowing for the testing of 600-1000 crystallization conditions in a single day using micrograms of protein, and facilitates structural and functional genomics, particularly in cancer research.

Problems solved by technology

Prior crystallization devices available for high throughput screening of crystallization conditions are incapable of addressing at least some of the features listed below.

Method used

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  • Microfluidic chip or biomolecule crystallization
  • Microfluidic chip or biomolecule crystallization
  • Microfluidic chip or biomolecule crystallization

Examples

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Embodiment Construction

[0021] The present invention provides a microstructured crystallization system that can be formed on a microfluidic chip. The crystallization system may be used to make single crystals from nanogram quantities of protein or other biomolecule, or to test 600-1000 crystallization conditions from microgram quantities of protein or other biomolecule. The present invention will be described below relative to an illustrative embodiment for crystallizing proteins. Those skilled in the art will appreciate that the present invention may be used to crystallize any desired biomolecule or create co-crystals of any combination of biomolecules and may be implemented in a number of different applications and embodiments. The present invention is not specifically limited in its application to the particular embodiments depicted herein.

[0022] The biomolecule crystallization system of the present invention integrates conventional membrane filter technology into a microfluidic chip to perform microdi...

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Abstract

A system for crystallizing a protein and other biomolecules comprises a flow channel for conveying a solution containing the biomolecule to be crystallized and one or more substations related to the crystallization process formed along the flow channel. The system operates by flowing a biomolecule solution through a the flow channel, then performing dialysis on a micro-scale to set crystallization conditions. After dialysis, the concentration of the biomolecule solution is changed by removing water or solvent from the biomolecule solution to promote formation of crystals in a segment of the flow channel. Formed crystals may be observed and harvested from the flow channel.

Description

RELATED APPLICATIONS [0001] The present application is a continuation of U.S. patent application Ser. No. 10 / 328,931, filed Dec. 23, 2002, entitled “Microfluidic Chip for Biomolecule Crystallization” which claims priority to U.S. Provisional Patent Application Ser. No. 60 / 427,620, filed Nov. 19, 2002, U.S. Provisional Patent Application Ser. No. 60 / 409,489 filed Sep. 9, 2002, and U.S. Provisional Patent Application Ser. No. 60 / 410,685, filed Sep. 13, 2002. The present application is related to U.S. patent application Ser. Nos. 10 / 028,852, 10 / 027,484, 10 / 027,516, 10 / 027,171 and 10 / 328,932. The contents of each of the foregoing applications are expressly incorporated herein by reference.FIELD OF THE INVENTION [0002] The present invention relates to a microfluidic system and method for crystallizing biomolecules, such as proteins. BACKGROUND OF THE INVENTION [0003] The understanding of specific properties, functions and three-dimensional structure of proteins is an invaluable asset for...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B01L3/00B01L3/06C30B7/00
CPCB01L3/0248Y10T436/255B01L3/502715B01L3/502723B01L3/502753B01L2200/0678B01L2300/0681B01L2300/087B01L2300/1827B01L2400/0487C30B7/00B01L3/06Y10T436/2525Y10T436/25C30B29/58
Inventor GILBERT, JOHNTRIKHA, JAISHREE
Owner GILBERT JOHN
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