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Assemble preparation method of DNA guided nano granule vertical type wire

A nanoparticle and nanometal particle technology, which is applied in the field of chemical self-assembly method to manufacture functional devices, can solve the problems of difficult regulation, difficult regulation and assembly, difficult precise control of DNA skeleton structure, etc., and achieve the effect of simple preparation method

Inactive Publication Date: 2010-12-15
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a problem with the DNA assembly of Seeman et al.: the size of the DNA assembly is about 10nm, and it is difficult to control and form a large-scale ordered assembly
Due to the electrostatic interaction between DNA and nanoparticles, it is difficult to control the assembly of the ordered multi-level structure of particle deposition during the preparation process. Another problem is that the DNA template uses λ-DNA, so the structure of the DNA backbone is difficult to precisely control. , easy to form a variety of DNA backbone structures

Method used

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  • Assemble preparation method of DNA guided nano granule vertical type wire
  • Assemble preparation method of DNA guided nano granule vertical type wire

Examples

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Effect test

Embodiment 1

[0017] A preparation method for DNA-guided nanoparticle assembly of vertical wires, consisting of the following steps: (1) placing an aqueous solution of gold particles with a volume ratio of 400:1 and a concentration of 200nM with an average particle diameter of 10 nanometers at a concentration of 50μM of the 5' end sulfhydryl modified deoxyribonucleic acid sequence SH-(CH 2 ) 6 -T 8 solution (T is thymidine), stirred for 4 hours, so that the surface of gold nanoparticles formed a DNA modification layer by interacting with sulfhydryl groups; (2) adding dimethyl sulfoxide to make dimethyl sulfoxide in the solution The concentration is 1.5mM, add NaCl-tris-HCl buffer solution to make the pH value 6; and add Na + and Mg 2+ so that the solution contains Na + The concentration is 30mM, Mg 2+ The concentration was 1.0 mM. (3) Place it at 20° C. for 18 hours to make a DNA-guided nanoparticle-assembled vertical wire.

Embodiment 2

[0019] A preparation method for DNA-guided nanoparticle assembly of vertical wires, consisting of the following steps: (1) placing an aqueous solution of gold particles with an average particle diameter of 10 nanometers at a concentration of 5 nM with a volume ratio of 100:1 0.3mM 5' end sulfhydryl modified deoxyribonucleic acid sequence SH-(CH 2 ) 4 -T 20 In the solution, stir for 6 hours, make the surface of the gold nanoparticles form a DNA modification layer by interacting with mercapto groups; (2) add trimethoxymercaptopropylsilane, so that the concentration of trimethoxymercaptopropylsilane in the solution is 3.5mM, Add tris-sodium borate buffer solution to make the pH value 8; and add Na + and Mg 2+ so that the solution contains Na + The concentration is 70mM, Mg 2+ The concentration is 0.5 mM. (3) Place it at 25° C. for 8 hours to make a DNA-guided nanoparticle-assembled vertical wire.

Embodiment 3

[0021] A preparation method for DNA-guided nanoparticle assembly of vertical wires, consisting of the following steps: (1) placing an aqueous solution of silver particles with an average particle diameter of 10 nanometers at a concentration of 5 nM with a volume ratio of 600:1 0.1mM 5' terminal sulfhydryl modified deoxyribonucleic acid sequence SH-(CH 2 ) 10 -T 25 In the solution, stir for 2 hours, make the surface of nano-silver particles form a DNA modification layer by reacting with mercapto groups; (2) add trichloroethyl phosphate, make the concentration of trichloroethyl phosphate in the solution be 1.5mM, add trichloroethyl phosphate hydroxymethylaminomethane-HCl buffer solution to make the pH value 6; and add Na + and Mg 2+ so that the solution contains Na + The concentration is 50mM, Mg 2+ The concentration is 0.8mM. (3) Place it at 20° C. for 18 hours to make a DNA-guided nanoparticle-assembled vertical wire.

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Abstract

The invention relates to a method for using DNA induction nanometer particles to form vertical wire, wherein said method comprises that: 1, putting solution with nanometer metal particles into deoxyribonucleic acid solution decorated by 5' mercapto to be mixed; 2, adding dimethyl sulfoxide, triethyl ester phosphate, or tripropoxy silicane, etc, adding buffer solution, adding Na+ and Mg2+, or adding K+ and Mg2+; 3, laying to form the tree wire. The invention uses the DNA recognize function, to induce the nanometer particles to form the nanometer vertical wire, via the DNA sequence and self-installment condition to adjust the vertical angles of branch and main tree of nanometer tree, and the distance between branch distance. The invention can be used in DNA molecule device, etc.

Description

technical field [0001] The invention belongs to the technical field of using a chemical self-assembly method to manufacture functional devices, and in particular relates to a preparation method for using DNA to regulate the assembly of nanoparticles to form regular wires. Background technique [0002] DNA is not only the most basic substance of life inheritance, but also its unique molecular structure and characteristics have attracted the attention of the research fields of materials and information science. The secondary structure of DNA achieves self-assembly at the nanometer level (the diameter of the DNA double helix structure is 2nm, and the pitch is 3.4nm), and it is easy to control and design artificially (the elements that make up the nucleic acid molecule are only four nucleotides). Through the synergistic interaction between DNA or DNA and other building units, combined with molecular recognition and self-assembly properties, the development of functional molecula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01B13/00B82B3/00
Inventor 李张金利张慧李
Owner TIANJIN UNIV
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