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Method for obtaining lily distant hybrid by using rescue isolated culture of buds

A technology for in vitro rescue of immature embryos and distant hybrids, applied in horticultural methods, botanical equipment and methods, applications, etc., to achieve the effects of avoiding premature aging, shortening the cultivation cycle and survival rate of hybrids, and shortening the breeding cycle

Inactive Publication Date: 2007-07-11
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, most lily species have good fragrance, wide habitat distribution, strong disease resistance and adaptability, and have certain advantages in improving fragrance, resistance, and increasing the genetic diversity of existing lily varieties. Further research is urgently needed. develop and use
At the same time, with the development of the flower industry in Yunnan Province for more than 10 years, hundreds of modern cultivated varieties have been introduced into Yunnan. These varieties have rich flower colors, flower types, different growth vigor, disease resistance, and stress resistance. It is suitable for the climate and soil conditions in Yunnan, and various excellent traits are well displayed. Some of them show certain advantages, but their shortcomings are also prominent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. From the 5th day of artificial pollination between Lilium species, the morphological development of the pollinated ovary embryo was observed, and according to the observation of ovary expansion and atrophy, the entire fertilized ovary of the distant hybridization 10 days after pollination was cut off , stored at 4°C for 12 hours, wipe the surface twice with 75% alcohol cotton balls, then soak and disinfect with 75% alcohol for 1 minute, and then soak in 0.1% mercuric solution for 15 minutes , shaken several times, and finally washed twice with sterile water, the contamination rate can be effectively controlled within 3%. Select the swollen part from the top to the middle of the sterilized ovary, and cut it into 0.2cm slices. Each ovary can be sliced ​​into 8-15 slices. At this time, in the placenta of the sliced ​​ovary, slightly transparent enlarged ovules can be seen with the naked eye;

[0037] 2. Under sterile conditions, inoculate the sterilized ovary slices int...

Embodiment 2

[0067] 1. From the 5th day of artificial pollination between Lilium species, observe the morphological development of pollinated ovary embryos, and cut off the entire fertilized ovary of distant hybridization 20 days after pollination according to the observation of ovary expansion and atrophy , stored at 2°C for 15 hours, wipe the surface with 75% alcohol cotton ball for 3 times, then soak and disinfect with 75% alcohol for 2 minutes, and then soak in 0.05% mercuric solution for 18 minutes , shaken several times, and finally washed twice with sterile water, the contamination rate can be effectively controlled within 3%. Select the swollen part from the top to the middle of the sterilized ovary, and cut it into 0.2cm slices. Each ovary can be sliced ​​into 8-15 slices. At this time, in the placenta of the sliced ​​ovary, slightly transparent enlarged ovules can be seen with the naked eye;

[0068] 2. Under sterile conditions, inoculate the sterilized ovary slices into the foll...

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Abstract

The invention relates to a method for rescuing blast to obtain remote hybrid of lily, wherein it selects YunNan wide lily and better system, uses lily hybrid as male, to cross; uses cross pollination and blast separation cultivation to take it out and cultivate before fail, to avoid earl decay, to grow remote hybrid blast into normal seed, to shorten the select period and improve survival rate.

Description

technical field [0001] The invention relates to a method for obtaining distant hybrids of the genus Lilium through in vitro culture of fertilized immature embryos, which belongs to the field of biological technology breeding. Background technique [0002] Lilium (Lilium L.) is one of the most important cut flower materials in the world because of its beautiful flower shape, noble and elegant, and pleasant aroma. There are more than 90 kinds of original species of Lilium, and the hybridization work is very active because of its rich flower colors. In the 1920s and 1930s, some countries began to study the interspecific hybridization of Lilium, and used distant hybridization to obtain interspecific lily hybrids. [0003] China is the natural distribution center of lily resources in the world. There are about 96 species of lily plants in the world, and 47 species are distributed in China (36 species are endemic to China), and there are 18 varieties in addition. There are more ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01N3/02
Inventor 屈云慧熊丽蒋亚莲吴学尉张艺萍
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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