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A black poplar pollen diploid induction method

A technology of diploid and black poplar, which is applied in the fields of botanical equipment and methods, plant genetic improvement, horticulture, etc., can solve the problems of difficulty in cultivating polyploids, and is not suitable for black poplar 2n pollen, etc., and achieves stable induction effect. The effect of increasing the ratio

Inactive Publication Date: 2011-03-16
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the flower buds do not disperse pollen after being induced by high temperature or the amount of disperse pollen is very small, and the obtained 2n pollen is abnormal conjoined pollen, it is difficult to meet the needs of cultivating polyploids
Therefore, the induction method of Mashkina in the former Soviet Union is not suitable for the induction of poplar 2n pollen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. From January 20 to February 20, the flower branches of Yang Xiong in Shanhaiguan were collected, and they were hydroponically grown in a greenhouse at a temperature of 20°C. During the hydroponics period, the water in the container was replaced every two days to keep the water for cultivation in the lower part of the flower branches clean;

[0018] 2. When the male flower buds on the flower branches develop to the leptotene stage of meiosis, inject a colchicine solution with a concentration of 0.8% into the flower buds for 4 times, and the interval between two adjacent injections is 3 hours.

[0019] 3. Continue to hydroponic the flower branches until the pollen is released.

[0020] The induction effect was that the ratio of 2n pollen was 87.4%, the amount of loose pollen per inflorescence was 0.41 times that of the control, and no 2n pollen was detected in the control pollen.

[0021] The following steps are taken to detect the 2n pollen ratio: count the proportion...

Embodiment 2

[0026] Except that the colchicine solution was injected into the flower buds when the male flower buds on the flower branches developed to the pachytene stage of meiosis, and the interval between two adjacent injections was 2 hours, the rest were the same as in Example 1.

[0027] The induction effect was that the ratio of 2n pollen was 59.15%, the amount of loose pollen per inflorescence was 0.62 times that of the control, and no 2n pollen was detected in the control pollen.

Embodiment 3

[0029] Except that the male flower bud on the flower branch develops to the final stage of meiosis, the colchicine solution is injected into the flower bud, and the interval between two adjacent injections is 2 hours, the rest are the same as in Example 1.

[0030] The induction effect was that the ratio of 2n pollen was 50.20%, the amount of loose pollen per inflorescence was 0.38 times that of the control, and no 2n pollen was detected in the control pollen.

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Abstract

The invention relates to a method for inducing diploid black Salicaceae pollen, wherein it comprises that: 1, collecting black Salicaceae at winter and spring into room temperature to be cultivated in water; 2, when the male bud grows fine line period and the final change period, ejecting colchicines solution into buds; 3, cultivating in water, until dispersing the pollen, the density of colchicines is 0.2-0.8%, the ejection times are 4-5, the ejection distance is 2-3h. The invention can improve 2n pollen ration as 40-95%, and the pollen yield can reach 0.32-2 times of former one.

Description

technical field [0001] The invention relates to a black poplar pollen induction method, in particular to a black poplar pollen diploid (2n) induction method. Background technique [0002] The practice of poplar cultivation and breeding in my country has proved that black poplar (section Aigeiros) is an important afforestation tree species and breeding parent resource for poplar plantations in my country, but the traditional black poplar improved breeding method centered on the use of interspecific hybridization to produce heterosis has passed For more than half a century, the volume of a single plant exceeds that of the control parent by less than 50% (Research Progress in Poplar Hybrid Breeding in China. World Forestry Research, Li Shanwen et al., 2004), and it is difficult to have a greater breakthrough. In order to make further use of the imported black poplar resources so that it can play a greater role in the construction of industrial timber forests and environmental pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01G1/00A01G31/00
Inventor 张金凤续九如卫尊征王丽华康向阳陈晓阳张志毅李云胡冬梅
Owner BEIJING FORESTRY UNIVERSITY
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