Perhydrolase

A technology of perhydrolase and perhydrolysis, applied in the direction of hydrolase, detergent compounding agent, biocide, etc., can solve problems such as difficult deinking and difficult removal of ink

Active Publication Date: 2011-06-08
GENENCOR INT INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While general methods are adequate for deinking paper with oil- or water-based inks, the increasing

Method used

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  • Perhydrolase
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[1357] Enzyme analysis

[1358] In this example, the methods used to assess enzyme purity and activity are described, which are used in the examples that follow and throughout the specification.

[1359] Enzyme activity assay (pNB assay)

[1360] The activity is determined by hydrolysis of p-nitrophenylbutyrate. The reaction mixture was prepared by adding 10ul of 100mM p-nitrophenylbutyrate in DMSO to 990ml of 100mM Tris-HCl buffer at pH 8.0 containing 0.1% triton X- 100. Background hydrolysis rates were measured at 410 nm prior to addition of enzyme. The reaction was initiated by adding 10 ul of enzyme to 990 ml of reaction mass and the change in absorbance at 410 nm was measured at room temperature (~23°C). Background corrected results are reported as ΔA 410 / min / ml or δA 410 / min / mg protein.

[1361] transesterification

[1362] Transesterification was determined by GC separation of the product in the buffered aqueous reaction. The reaction for the determination of...

Embodiment 2

[1370] Determination of the ratio between peracid formation and acid formation

[1371] In this example, a method for determining the ratio of perhydrolysis to hydrolysis is described. Specifically, this example provides a method for determining the ratio between peracid formation (i.e., perhydrolysis) and acid formation (i.e., hydrolysis) in an aqueous system in the presence of peroxide In the case of , produced by the activity of an enzyme on an ester substrate.

[1372] A. Determination of the ratio of perhydrolysis to hydrolysis

[1373] Substrate preparation

[1374] Substrates were prepared as described here. Ethyl acetate (EtOAc) and other water-soluble esters were diluted in the desired buffer to achieve an ester concentration of 10 mM. Tributyrin and other water insoluble substrates were prepared by preparing substrate swatches. Polyester samples were cut from undyed polyester fabric (Polycotton, PCW22) with a 5 / 8 inch punch and placed in 24-well microtiter pla...

Embodiment 3

[1409] Analysis of detergent-containing compositions

[1410] In this example, an assay system for screening for superior perhydrolase activity in detergents with specific substrates is provided. These assays include those that measure peracid degradation by perhydrolases as well as peracid synthesis activity of the enzymes.

[1411] Materials and Methods for Peracid Formation (PAF) and Peracid Degradation (PAD) Analysis

[1412] This section provides materials and methods for screening better perhydrolases with C9E2OAC ester substrates in Ariel.

[1413] Material:

[1414] Ariel Futur (P&G, Ariel "C") Free of Bleach, Fragrance and Enzyme

[1415] C9E2OAc (P&G)

[1416] 30% hydrogen peroxide (Sigma)

[1417] 32% peracetic acid ("peracid", PAA) (Sigma cat#) MW=76.05; 4.208M

[1418] Acetic acid, anhydrous MW=192.12

[1419] Potassium hydroxide MW=56.11

[1420] ABTS (Sigma cat# A1888) MW=548.68

[1421] Potassium iodide MW=166.0

[1422] Potassium phosphate, monobas...

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Abstract

The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.

Description

[0001] This application claims priority to co-pending US Provisional Patent Application Serial No. 60 / 526,764, filed December 3, 2003, under 35 U.S.C. §119. technical field [0002] The present invention provides methods and compositions comprising at least one perhydrolase for cleaning and other uses. In some particularly preferred embodiments, the present invention provides methods and compositions for producing peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfection. Background technique [0003] Detergents and other cleaning compositions typically include complex combinations of active ingredients. For example, most cleaning products include surfactant systems, enzymes for cleaning, bleaches, builders, suds suppressors, soil-suspending agents, soil-suspending agents -release agents), optical brighteners, softeners, dispersants, dye transfer inhibiting compounds, abrasives, fungicides and fragrances. Despite t...

Claims

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Application Information

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IPC IPC(8): C12N9/16C12N9/50C12N9/52A01N63/50C11D3/386
CPCC12N9/50C11D3/38636C12N9/52C12N9/16A01N63/00A01N63/50A01N63/20
Inventor N·S·阿明M·G·波士顿R·R·博特M·A·切尔温E·M·孔卡M·E·古斯特威勒B·E·约翰斯K·利贝顿G·S·米拉克莱H·吴A·J·波罗斯S·W·拉默J·J·沙伊贝尔W·韦勒G·M·怀特德
Owner GENENCOR INT INC
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