Inclined type gravity precipitation live cell trapping device
A technology of gravity sedimentation and interception devices, which is applied to tissue cell/virus culture devices, biochemical instruments, microorganisms, etc., can solve the problems of reactor environment deterioration, long residence time of living cells, cell damage, etc., and reduce deposition and adhesion. Attached, the effect of shortening the average residence time and alleviating the overall activity decline
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[0049] This settler is used in combination with 1L stirred cell culture reactor for perfusion culture of hybridoma cells.
[0050] Cell: The cell line is hybridoma cell HB-58, which is deposited in ATCC (American Tissue Cell Bank) with the preservation number Hybridoma CELL Line 187.1 (ATCC HB58). Cells were maintained in 300ml spinner bottles at 37°C with 5% CO 2 Inoculate the reactors during the logarithmic phase of the culture in an incubator.
[0051] The culture medium is Hybridoma-SFM (1X) (serum-free medium for culturing hybridoma cells) from GIBCO Company, the serial number of which is 12045-035.
[0052] The angle α between the liquid receiving port 3 and the bottom surface of the main body 1 is 45°;
[0053] When in use, the main body 1 is tilted, and the angle β with the horizontal direction is 50°
[0054] Continuous perfusion culture for 60 days. Using glucose-limited medium (glucose concentration 7 mM, glutamine 8.5 mM), at a perfusion rate of 1 / day, the maxi...
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