Inclined type gravity precipitation live cell trapping device

A technology of gravity sedimentation and interception devices, which is applied to tissue cell/virus culture devices, biochemical instruments, microorganisms, etc., can solve the problems of reactor environment deterioration, long residence time of living cells, cell damage, etc., and reduce deposition and adhesion. Attached, the effect of shortening the average residence time and alleviating the overall activity decline

Inactive Publication Date: 2007-05-23
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a downward inclined gravity sedimentation live cell interception device used in the continuous long-term perfusion culture process of animal cells, to solve the problem that the average residence time of living cells in the settler is long, causing cell damage and reactor environment. worsening problem

Method used

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  • Inclined type gravity precipitation live cell trapping device
  • Inclined type gravity precipitation live cell trapping device

Examples

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Embodiment 1

[0049] This settler is used in combination with 1L stirred cell culture reactor for perfusion culture of hybridoma cells.

[0050] Cell: The cell line is hybridoma cell HB-58, which is deposited in ATCC (American Tissue Cell Bank) with the preservation number Hybridoma CELL Line 187.1 (ATCC HB58). Cells were maintained in 300ml spinner bottles at 37°C with 5% CO 2 Inoculate the reactors during the logarithmic phase of the culture in an incubator.

[0051] The culture medium is Hybridoma-SFM (1X) (serum-free medium for culturing hybridoma cells) from GIBCO Company, the serial number of which is 12045-035.

[0052] The angle α between the liquid receiving port 3 and the bottom surface of the main body 1 is 45°;

[0053] When in use, the main body 1 is tilted, and the angle β with the horizontal direction is 50°

[0054] Continuous perfusion culture for 60 days. Using glucose-limited medium (glucose concentration 7 mM, glutamine 8.5 mM), at a perfusion rate of 1 / day, the maxi...

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Abstract

The invention discloses a clinism gravitational settling living cell trapping equipment, which contains tilted settling vessel main body (1), feeding throat (2), liquid receiving mouth (3), and refluxing opening (4). It is characterized in that it contains ribs strips (6). Cross section of the said settling vessel main body is oval shape, feeding throat (2) is set on superior extremity of the main body (1), liquid receiving mouth (3) is set at the back of connecting piece of the main body (1), refluxing opening (4) is set at inferior extremity of the main body. The said ribs strips are set in the bottom of the internal surface of the main body. The trapping equipment of this invention is fit for different reactor and has a wide dabbling rate scope. The invention reduces the deposition of cells at bottom of the settling vessel, shortens the mean detention time of cells outside the reactor, and relieves the entire activity descend and environmental aggravation caused by cell deposition.

Description

technical field [0001] The invention relates to an inclined gravity settling living cell intercepting device, in particular to an inclined gravity settling device used for selectively retaining living cells in the continuous perfusion culture process of cells. Background technique [0002] Perfusion culture is an effective way to increase cell density. The advantage of this culture method is that it can continuously add fresh medium without changing the volume of the culture medium, discharge metabolites, make the cells grow rapidly and maintain high activity, so as to achieve high expression and high yield of products. It is a key technical issue to implement continuous perfusion culture to develop a cell interception device and efficiently retain cells. [0003] Among the many cell retention devices, the inclined gravity settling device is considered to be a better cell retention method because of its simple structure, low cost, no clogging, small shearing of cells, and e...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/06
Inventor 谭文松张璐张旭
Owner EAST CHINA UNIV OF SCI & TECH
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