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Full cell catalyst of intracellular secretory lipase type and its preparing method

A whole-cell catalyst and endocrine technology, applied in the direction of biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of no repeated use, achieve the effects of saving equipment investment, easy recycling, and reducing production costs

Inactive Publication Date: 2007-04-11
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, Zeng Jing et al. from Tsinghua University used molds to dry and freeze directly to participate in the reaction practice, but there were no reports of repeated use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method for intracellular lipase-type whole-cell catalyst, which comprises the steps of:

[0031] 1). Selection of strains and culture medium: (1) Strains: the waste oil from the oil factory was placed in the medium for separation by the dilution plate method for separation and culture. The medium for separation was potato sucrose medium, and the cells produced Yeast (Candidalipolytica lipolytica) of endocrine lipase (see "Agricultural Microbiology Experimental Technology" page 69-72 for the isolation method, China Agricultural Press, May 1996, first edition); wherein potato sucrose medium Method: cut 200g potatoes into small pieces and boil them into juice, dissolve potato juice and 20g sucrose in 1L water, add 20g agar, sterilize at 121°C for 15min, and obtain potato sucrose medium; (2) Fermentation medium : Soybean oil 29.0g / L, sucrose 1.0g / L, peptone 70.0g / L, NaNO 3 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, sterilized at 121°C for 20 minute...

Embodiment 2

[0039] A preparation method for intracellular lipase-type whole-cell catalyst, which comprises the steps of:

[0040] 1). Selection of strains and culture medium: (1) Strains: the waste oil from the oil factory was placed in the medium for separation by the dilution plate method for separation and culture. The medium for separation was potato sucrose medium, and the cells produced Yeast (Candidalipolytica lipolytica) of endocrine lipase (see "Agricultural Microbiology Experimental Technology" page 69-72 for the isolation method, China Agricultural Press, May 1996, first edition); wherein potato sucrose medium Method: cut 200g potatoes into small pieces and boil them into juice, dissolve potato juice and 20g sucrose in 1L water, add 20g agar, sterilize at 121°C for 15min, and obtain potato sucrose medium; (2) Fermentation medium : Soybean oil 29.0g / L, sucrose 1.0g / L, peptone 70.0g / L, NaNO 3 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, sterilized at 121°C for 20 minute...

Embodiment 3

[0048] A preparation method for intracellular lipase-type whole-cell catalyst, which comprises the steps of:

[0049] 1). Selection of strains and culture medium: (1) Strains: the waste oil from the oil factory was placed in the medium for separation by the dilution plate method for separation and culture. The medium for separation was potato sucrose medium, and the cells produced Yeast (Candidalipolytica lipolytica) of endocrine lipase (see "Agricultural Microbiology Experimental Technology" page 69-72 for the isolation method, China Agricultural Press, May 1996, first edition); wherein potato sucrose medium Method: cut 200g potatoes into small pieces and boil them into juice, dissolve potato juice and 20g sucrose in 1L water, add 20g agar, sterilize at 121°C for 15min, and obtain potato sucrose medium; (2) Fermentation medium : Soybean oil 29.0g / L, sucrose 1.0g / L, peptone 70.0g / L, NaNO 3 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, sterilized at 121°C for 20 minute...

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Abstract

The present invention relates to one kind of full cell catalyst with lipase activity and its preparation process. The intracellular lipase secreting full cell catalyst is prepared with the materials including saccharomycetes 0.7-1.2 wt%, diatomite 2.1-3.6 wt%, sodium alginate 2.0-2.3 wt%, calcium dichloride 0.5-0.8 wt%, and pH 7.7 Na2HPO4-NaH2PO4 buffer solution of 92.1-94.7 wt%, with the saccharomycetes to diatomite weight ratio being 1 to 3. The intracellular lipase secreting full cell catalyst is used to replace lipase in producing biological diesel oil, and has simple preparation process, easy product recovery, reusability and low production cost.

Description

technical field [0001] The invention relates to a whole-cell catalyst with lipase activity and a preparation method thereof. Background technique [0002] In the production of biodiesel, the biggest obstacle to the large-scale production of lipase as a catalyst is the high production cost of lipase, which makes it difficult to realize large-scale industrial production and application. Biological whole cells producing lipase are used as catalysts (referred to as whole cell catalysts). After the lipase-producing microbial strains are fermented and cultured, the bacteria are collected and replaced by lipase after simple treatment to participate in the reaction of synthesizing mono-fatty acid methyl esters. The whole-cell catalyst eliminates the complicated processes of separation, extraction, purification and immobilization of lipase, and is one of the effective ways to overcome the current high cost of lipase production and application. [0003] Biological whole-cell catalyst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N11/00C12N9/20
Inventor 江木兰胡小加晏立英张银波
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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