Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Genetic marker linked to gene locus involved in barley resistance to yellow mosaic disease and use thereof

A technology of genetic markers and gene loci, applied in the fields of application, angiosperms/flowering plants, botanical equipment and methods, etc., can solve problems such as difficult to identify and select genes

Inactive Publication Date: 2007-03-21
JAPAN SCI & TECH CORP
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, barley yellow mosaic resistance is often associated with multiple loci, so it is difficult to confirm whether the genes (gene loci) related to barley yellow mosaic resistance have been accurately selected by methods such as crossing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetic marker linked to gene locus involved in barley resistance to yellow mosaic disease and use thereof
  • Genetic marker linked to gene locus involved in barley resistance to yellow mosaic disease and use thereof
  • Genetic marker linked to gene locus involved in barley resistance to yellow mosaic disease and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0221] [Example 1: QTL Analysis on Barley Yellow Mosaic Resistance]

[0222] In the algorithm of QTL analysis, simple interval mapping (simple interval mapping, hereinafter referred to as "SIM") and composite interval mapping (hereinafter referred to as "CIM") are used, and the analysis software uses MAPMAKER / QTL and QTL Cartographer performed. The threshold value of the LOD score was set to 2, and when the LOD score exceeded 2, it was estimated that the QTL existed at the position with the largest LOD within the two marker intervals.

[0223] [result]

[0224] Table 1 shows the results of the DHHS group, Table 2 shows the results of the RI1 group, and Table 3 shows the results of the RI2 group. It should be noted that the "group" in the above Tables 1 to 3 refers to the "name of the group for which QTL analysis has been performed", "character" refers to the "character", "chromosome" refers to the "chromosome where the genetic marker is located", and " Marker interval (M1-A...

Embodiment 2

[0255] [Example 2: Detection of genetic marker "FEggaMtgg116"]

[0256] (method)

[0257] 50 ng of genomic DNA of barley to be tested was double-digested with 1.5 U each of EcoRI (manufactured by TAKARABIO) and MseI (manufactured by NEW ENGLAND Biolabs) in a total of 25 μl of reaction system at 37° C. for 12 hours. On the DNA after the restriction endonuclease treatment, with 5 μ M EcoRI linker (base sequence shown in SEQ ID NO: 49 and SEQ ID NO: 50) and 50 μ M MseI linker (base sequence shown in SEQ ID NO: 47 and SEQ ID NO: No. 48) Ligation was performed at 37° C. for 3 hours with 25 U of T4 ligase (manufactured by TAKARABIO). Pre-amplification (preliminary amplification) was performed on the ligated DNA fragments using EcoRI universal primer (base sequence shown in SEQ ID NO: 52) and MseI universal primer (base sequence shown in SEQ ID NO: 51). In a 0.07 mg / ml preamplification reaction solution, an amplification reaction was performed using an EcoRI selection primer having...

Embodiment 3

[0264] [Example 3: Detection of genetic marker "FEgggMcaa585"]

[0265] The method is to perform an amplification reaction (this amplification) using an EcoRI selection primer having the nucleotide sequence shown in SEQ ID NO: 38 and a MseI selection primer having the nucleotide sequence shown in SEQ ID NO: 37. , using the same method as that shown in the above-mentioned embodiment 2.

[0266] (result)

[0267]Figure 13 shows the results of electrophoresis of the amplified fragments obtained by the above amplification reaction. The left and right lanes in Figure 13 represent size markers, and the second lane from the left lane in the same figure shows the results of the above-mentioned AFLP detection on the barley variety Russia6 resistant to barley yellow mosaic disease, and the second lane from the left end lane in the same figure Lane 3 shows the results of the above-mentioned AFLP detection on the barley variety H.E.S.4 suffering from barley yellow mosaic disease. The o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Through creation of a detailed linkage map of barley and QTL analysis thereof, there have been found five genetic markers linked to gene locus involved in barley resistance to yellow mosaic disease and situated on barley 1H chromosome, two genetic markers linked to gene locus involved in barley resistance to yellow mosaic disease and situated on barley 2H chromosome barley resistance to yellow mosaic disease, five genetic markers linked to gene locus involved in barley resistance to yellow mosaic disease and situated on barley 3H chromosome, four genetic markers linked to gene locus involved in barley resistance to yellow mosaic disease and situated on barley 4H chromosome, and two genetic markers linked to gene locus involved in barley resistance to yellow mosaic disease and situated on barley 5H chromosome.

Description

technical field [0001] The present invention relates to a new genetic marker and its utilization method, in particular to a genetic marker linked to a gene locus related to resistance to barley yellow mosaic disease of barley and its utilization. Background technique [0002] Barley yellow mosaic disease is caused by barley yellow mosaic virus (hereinafter referred to as "BaYMV") or barley mild mosaic virus (hereinafter referred to as "BaMMV") as the causative virus, and algae A soil-borne viral disease in which Polymyxagraminis of the fungus is a mediator. Once the disease occurs, it will cause necrotic spots or yellowing of leaves, reduction of ramets, stunting, death, etc., and once the disease occurs, even if the soil is not cultivated for 4 to 5 years, it will not be free from disease, so it has become a serious problem. In particular, barley for beer is prone to disease. As the cultivation of barley for beer increases, it occurs not only in Japan but also in China and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/09A01H5/00C12Q1/68
Inventor 武田和义佐藤和广
Owner JAPAN SCI & TECH CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products