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Correlation analysis of circulation endothelium progenitor cell and coronary artery pathological changes

A technology for coronary artery disease and endothelial progenitor cells, which is applied in the field of molecular biology and can solve problems such as unproven coronary artery disease correlation.

Inactive Publication Date: 2007-03-14
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although some people speculate that the number of EPCs has a certain correlation with endothelial cell function and cardiovascular risk factors, the correlation between the number and function of EPCs and coronary artery lesions has never been confirmed before the present invention

Method used

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  • Correlation analysis of circulation endothelium progenitor cell and coronary artery pathological changes
  • Correlation analysis of circulation endothelium progenitor cell and coronary artery pathological changes
  • Correlation analysis of circulation endothelium progenitor cell and coronary artery pathological changes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] method

[0045] 1. Research object

[0046] From March to May 2004, 104 inpatients who underwent coronary angiography (CAG) were studied (CAG positive or negative). Patients with malignant tumors, peripheral vascular disease, and proliferative retinopathy were excluded; patients with acute coronary syndrome, acute myocardial infarction, inflammation, acute bleeding or blood transfusion history, and patients taking statins or erythropoietin within the past 2 months were excluded; Premenopausal women were excluded.

[0047] 2. General clinical data

[0048] Collect basic information of all patients: gender, age, height, weight, blood pressure. Detailed medical history: hypertension, diabetes, coronary heart disease, cerebrovascular disease, kidney disease, smoking history, drinking history, lifestyle (whether to exercise), family history of coronary heart disease. And make routine laboratory tests: fasting and postprandial blood glucose, total cholesterol, low-density...

Embodiment 2

[0107] Screening for accelerators that boost EPC numbers

[0108] Take 10ml of fasting peripheral blood, and obtain mononuclear cells by density gradient centrifugation within 4 hours. Combine the same amount (approximately 1×10 5 ) mononuclear cells were inoculated on 6 culture plates coated with fibronectin (FN) (BD Biosciences, CA, USA).

[0109] In the 3 culture plates of the control group, culture in EGM medium (containing 10% fetal bovine serum, penicillin 100U / ml, streptomycin 100U / ml) (Cambrex, Walkersville, MD) without adding any compound to be tested 4 days.

[0110] In the 3 culture plates of the control group, in the EGM medium (containing 10% fetal bovine serum, penicillin 100U / ml, streptomycin 100U / ml) (Cambrex, Walkersville, MD) with a concentration of 0.1M of the compound to be tested ) for 4 days.

[0111] After 4 days of culture, wash off the non-adherent cells in the test group and the control group with PBS, change the culture medium and continue to cul...

Embodiment 3

[0115] Screening for compounds that promote the ability of circulating endothelial progenitor cells to migrate and adhere

[0116] For the promoters screened in Example 2 to promote the number of EPCs, it was further determined whether they have the ability to promote the migration and adhesion of circulating endothelial progenitor cells:

[0117] (a) Detection of migration ability of EPCs

[0118] Adherent cells were collected and counted as in Example 2. Put 600 μl culture solution and vascular endothelial growth factor (VEGF, 50ng / ml) into the lower chamber of transwell (8-μm pore size) (Corning Costar Inc., Corning, New York, USA), and put 2×10 4 EPCs were suspended in 200 μl of culture solution and injected into the upper chamber, cultured for 24 hours, scraped off the immobile cells on the filter membrane, fixed with 75% ice ethanol, stained with HE, and randomly selected 5 microscope fields (×200) to count the cells that migrated to the lower layer.

[0119] (b) Dete...

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Abstract

The present invention discloses the correlation between circular endothelial progenitor cell (EPC) and coronary artery pathological change. It is test proved that the number and migration and adhesion power of circular EPC is high correlated with the stenosis degree of coronary artery, so that circular EPC may be used as the reference index for diagnosing the stenosis degree of coronary artery. The present invention also provides method and kit for detecting the stenosis degree of coronary artery based on the said correlation.

Description

technical field [0001] The present invention relates to the field of molecular biology. More specifically it relates to the correlation between circulating endothelial progenitor cells and coronary artery disease, especially the degree of coronary artery stenosis. The invention also relates to a method and a kit for detecting coronary artery lesions based on the correlation. Background technique [0002] Coronary artery disease is a disease that seriously endangers people's health. [0003] Since 1997, Asahara et al. successfully induced human peripheral blood CD34 in vitro for the first time + Since differentiation of cells into endothelial cells (Asahara T, Murohara T, Sullivan A. et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science 1997; 275:964-7), there has been increasing evidence for the presence of endothelial progenitor cells in the adult bone marrow (EPCs), when blood vessels are injured, bone marrow EPCs are mobilized to periphera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00A61K45/00A61P9/10
Inventor 朱鼎良高平进汪海娅张怡周晓鸥
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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