Chlorine resistant strain V430 and its screening method

Abstract

The present invention relates to a kind of chlorine tolerant microbe and its screening process. The chlorine tolerant microbe strain is Staphylococcus saprophyticus V430 in the preservation number of CCTCC No. M205145. The Staphylococcus saprophyticus V430 has circular opaque colony shape with lug and smooth edges; spherical single, paired or piled cells of d in 0.6-0.9 micron without flagellum; no motion, no endogeneous spore, gram positive and anaerobic property; and optimal growth pH value of 6.0-7.5 and temperature of 25-45 deg.c. It can survive in high chlorine ion concentration environment and degrade high concentration organic pollutant effectively.

Description

technical field [0001] The invention relates to a chlorine-resistant microorganism and a screening method thereof. Background technique [0002] High-salinity refractory organic wastewater, such as petroleum exploration, chemical, pharmaceutical and other wastewater, has become a worldwide technical problem in the field of environmental protection water treatment. However, the hydrochloric acid method turmeric saponin production wastewater is representative of the high-concentration saline refractory organic wastewater. On the one hand, this type of wastewater has complex components, many refractory organic substances, and poor biodegradability of the wastewater; on the other hand, the wastewater has a high salt content and a high concentration of chloride ions, and the concentration of chloride ions in the mixed wastewater can reach 20,000 mg / L. At present, non-biological methods and biological methods are used for saline organic wastewater. The non-biological method is un...

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Problems solved by technology

On the one hand, this type of wastewater has complex components, many refractory organic substances, and poor biodegradability of the wastewater; on the other hand, the wastewater has a high salt content and a high concentration of chloride ions, and the concentration of chloride ions in the mixed wastewater can reach 20,000 mg / L

At present, non-biological methods and biological methods are used for salt-containing organic wastewater. The non-biological method is unacceptable to enterprises due to its high treatment cost and poor treatment effect. The biochemical method has always been the first method in the water treatment process, mainly using A-B two-stage contact Oxidation method, traditional activated sludge method, SBR method, biological filter, anaerobic filter, etc., but most of the above biological methods can only treat wastewater with a salt content of less than 3%, while for high-salt wastewater (salt content 5% %, even 20%) are difficult to handle and therefore require special microorganisms

In recent years, foreign countries have reported reports on the breeding of salt-tolerant (NaCl) bacteria to treat high-salt organic wastewater. Complex wastewater is still a weak field of research

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