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Nanometer decalcified bone matrix material for bone regeneration and its prepn

A technology for demineralized bone matrix and bone regeneration, applied in the field of bioengineering, can solve the problems of reduced bone support and biomechanical properties, and achieve the effect of improving performance

Inactive Publication Date: 2007-01-03
上海再生医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the bone conduction effect of non-nanometer decalcified bone matrix is ​​weaker than that of autologous bone; after decalcification treatment, its biomechanical properties decrease, and the bone supporting effect during bone graft fusion decreases; There is a certain degree of rejection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In the present invention, the nano bone regeneration material is prepared by the following method:

[0019] 1. Use fresh healthy adult bones, remove soft tissues to obtain bone pieces of different sizes, rinse with running water for 4hr, dehydrated by absolute ethanol for 2hr, degreasing with ether for 1.5hr, dry in a ventilated place, freeze-dried in the refrigerator at -80℃, and use a sample mill Crush into 0.5mm particles;

[0020] 2. Soak in absolute ethanol for 2hr, ether soak for 2hr, rinse 3 times with distilled water, then soak in hydrochloric acid for 6hr, ether soak for 1.5hr, dry in a ventilated place to obtain decalcified bone matrix DBM (calcium residue weight ratio is 1%), and then Lyophilize in the refrigerator at -80℃;

[0021] 3. Grind the DBM to 50nm nano particles for 10hr with a 100 rpm grinder, and then sterilize with gamma rays or ethylene oxide to obtain the nano bone regeneration material.

Embodiment 2

[0023] In the present invention, the nano bone regeneration material is prepared by the following method:

[0024] 1. Use fresh healthy adult bone, remove soft tissues to obtain bone pieces of different sizes, rinse with running water for 4hr, dehydrated by absolute ethanol for 1hr, defatted with ether for 2hr, dry in a ventilated place, freeze-dried in the refrigerator at -80℃, and crushed into 1.5mm particles;

[0025] 2. Soak in absolute ethanol for 3hr, soak in ether for 1hr, rinse twice with distilled water, soak in hydrochloric acid for 4hr, soak in ether for 1hr, dry in a ventilated place to obtain decalcified bone matrix DBM (calcium residue weight ratio is 3%), put it at -30 ℃ Refrigerator freeze-drying;

[0026] 3. Grind the DBM to 100nm nano-particles with a 500 rpm grinder for 6 hours, and then sterilize it with gamma rays or ethylene oxide to obtain nano bone regeneration materials.

Embodiment 3

[0028] In the present invention, the nano bone regeneration material is prepared by the following method:

[0029] 1. Use fresh healthy adult bones, remove soft tissues to obtain bone pieces of different sizes, rinse with running water for 2 hours, dehydrated with absolute ethanol for 3 hours, degreasing with ether for 1 hour, dry in a ventilated place, freeze-dried in a refrigerator at -80°C, and crushed into a sample mill 2.5mm particles;

[0030] 2. Soak in absolute ethanol for 1hr, soak in ether for 3hr, rinse 5 times in distilled water, soak in hydrochloric acid for 2hr, soak in ether for 2hr, dry in a ventilated place to obtain demineralized bone matrix DBM (calcium residue weight ratio is 5%), put it at -60 ℃ Refrigerator freeze-drying;

[0031] 3. Grind the DBM to 10nm nano-particles with a 900 rpm grinder for 8 hours, and then sterilize it with gamma rays or ethylene oxide to obtain nano bone regeneration materials.

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Abstract

The present invention belongs to the field of one kind of nanometer decalcified bone matrix (DBM) material with weak antigenicity and good osteogenesis inducing activity for bone regeneration and its preparation process. The preparation process includes the following steps: 1. obtaining fresh health adult bone, eliminating soft tissue, obtaining bone blocks of different sizes, flushing in flowing water for 2-6 hr, dewatering with anhydrous alcohol for 1-3 hr, defatting with ethyl ether for 0.5-2 hr, air drying, freeze drying at -10 to -100 deg.c in a refrigerator and grinding in a mill into 0.5-2.5 mm size grains; 2. soaking in anhydrous alcohol for 1-3 hr soaking in ethyl ether for 1-3 hr, rinsing with distilled water for 2-5 times, soaking in hydrochloric acid for 2-8 hr, soaking in ethyl ether for 0.5-2 hr, air drying to obtain DBM and freeze drying at -10 to -100 deg.c in a refrigerator; and 3. grinding DBM in a grinder to 1-300 nanometers and gamma ray or ethylene oxide sterilizing.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a nano bone regeneration material and a preparation method thereof, in particular to a nano decalcified bone matrix material used for bone regeneration and a preparation method thereof. Background technique [0002] Since ancient times, bone nonunion, bone defect and osteonecrosis have been a major problem faced by orthopedic surgeons. Autologous bone is an ideal bone graft material for workers, but it has obvious shortcomings, such as: 1. The source is limited, and it cannot meet the needs of large-scale bone defects and various revision operations, especially for pediatric patients; 2. Donor site complications The extra pain from 5% to 25% has been reported, mainly including: blood vessel and nerve damage, fracture, hematoma, infection and chronic pain, etc.; 3. The operation time is prolonged, and the amount of bleeding is correspondingly increased. Clinical application of bon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/50
Inventor 张忠民陈雄生
Owner 上海再生医疗科技有限公司
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