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A plant quick propagation method

A plant and fast technology, applied in the direction of botany equipment and methods, plant cells, plant regeneration, etc., can solve problems such as difficult to return to natural soil conditions, uncertain rooting possibility, and limited application range, so as to improve plant quality, Effect of reduced plant loss, reduced use of rooting plant growth regulators

Inactive Publication Date: 2006-12-13
吕平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The application scope of the non-test tube rapid propagation technology in the prior art is limited, such as the aerosol rapid propagation rooting technology, the root system is mostly water rooting, the plant mechanism characteristics are dependent, it is difficult to return to the natural conditions of the soil, and the transplanting survival rate is low;
[0007] The known non-test tube high-efficiency and rapid propagation technology is actually reflected in the integration of modern cutting technology. It is a simple technology to solve the rapid rooting of herbaceous plants. It can be completed by using rooting agents and a little management of the growth environment; but for woody plants, the vascular bundles and root systems are connected. A certain process is required, and the possibility of rooting in 4-11 days is uncertain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 The rapid propagation process of Rhododendron

[0046] Establish a sugar-free sterile seedbed with perlite as a substitute for tissue culture test tubes (e.g. figure 1 Shown), the bottom of a turtle-shaped seedbed for easy drainage (such as Picture 1-1 Shown), the negative electric field network that generates the electric field (such as Figure 1-2 Shown), the bottom layer of pebbles to increase permeability (as Figure 1-3 Shown), a magnetizer that generates a magnetic field (such as figure 1 -4) and the perlite matrix layer (such as figure 1 -5) composition. The seedbed is 120 cm wide and 25 cm high. Control the substrate humidity to 60%, take the plant section of the yellow rhododendron with leaves as the in vitro material, use indole butyric acid (IBA) with an oxygen content of 18 grams per liter and a concentration of 220 ppm, at a field strength of 40,000 volts / cm, and a frequency of 30KHz Treated under the alternating electric field for 60 minutes, mul...

Embodiment 2

[0051] Example 2 Rapid breeding process of hybrid hazelnut

[0052] The construction of the seedbed was the same as in Example 1, except that the humidity of the substrate was controlled to 80%. The frame structure and installation of the small arch shed and the installation of the nutrient solution delivery pipe are the same as those in the first embodiment.

[0053] Take the plant section of the hybrid hazelnut with leaves as the in vitro material, use 21 grams of oxygen per liter, indole butyric acid (IBA) with a concentration of 220 ppm, and treat it under an alternating electric field with a field strength of 80,000 volts / cm and a frequency of 20KHz. Minutes, multiplied in the seedbed. This IBA solution not only rapidly revives the cells of the in vitro material at this oxygen concentration, but also increases the tissue activity by 4 times. Under the electric field, the cells and IBA elements are both active, and the effect rate is increased by 5 times.

[0054] Through vent...

Embodiment 3

[0059] Example 3 The rapid breeding process of Rhododendron

[0060] The construction of the seedbed was the same as in Example 1, except that the seedbed was 135 cm wide and 30 cm high. Kaolinite with a diameter of less than 0.3 cm is selected as the substrate, and the humidity of the substrate is controlled to 70%. The frame structure of the small arch shed, the installation of facilities and the installation of the nutrient solution delivery pipe are the same as those in the first embodiment.

[0061] Take the plant section of the yellow rhododendron with leaves as the in vitro material, use indole butyric acid (IBA) with an oxygen content of 21 grams per liter and a concentration of 180 ppm, and treat it under an alternating electric field with a field strength of 60,000 volts / cm and a frequency of 25KHz. Minutes, multiply on the seedbed This IBA solution not only rapidly revives the cells of the in vitro material at this oxygen concentration, but also increases the tissue act...

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Abstract

The invention relates to a kind of plant rapid breeding method by utilizing plant re-tube non-sugar artificial heterotrophy procedure for supporting light auto tropic and realizing mixotrophic tissue culture, which comprises the follow procedures: built seedling bed in greenhouse canopy or field, built small hump hut and cover ventilating membranella; take out the plant segment that have leave or one-bud-one-leave as exsomatize material, use indolebutyric acid solution that every liter contain18-21 gram oxygen, the consistence is 110-220 ppm, treat 30-90 minutes, breed in the seedling bed; control the temperature, the air humidity, matrix humidity, sunshine, carbon dioxide consistence, disinfect, the entire valence inorganic nutrition liquor pass through the magnetic field that the magnetic flux is 0.4-0.6T or the alternating electric field that the density of field energy is 6-10KV / cm, the frequency is 15-26KHz,the nutrition liquor flux is 0.2 liter / s, after proceed magneticsation or electrolytic treatment activating, sprinkle the nutrition liquor.

Description

Technical field [0001] The present invention belongs to the field of biotechnology. Specifically, the present invention is a method for rapid plant breeding that supports photoautotrophism by a non-test-tube sugar-free artificial heterotrophic program for plants and realizes cultivating ectopic tissues. Background technique [0002] Seed breeding, most of the seedlings cultivated by seed retention have the separation of offspring traits, resulting in uneven agronomic traits and yield quality, affecting commercial or biological traits, and it is difficult to use in production, and it is difficult to expand in a short period of time. A large number of commercial seedlings with the same traits are produced, and the offspring have large segregation and variation. They are very prone to damping-off in the seedling stage, with long cycles and slow speed. [0003] Cutting seedlings are restricted by seasons and can only be carried out in spring or autumn when the climate is suitable. Th...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G7/04
CPCY02P60/14
Inventor 吕平
Owner 吕平
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