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Method for preparing purity astragaloside

A technology of astragaloside IV and pure product, applied in the field of medicine, can solve the problems of unsuitability for industrial production, difficult extraction and separation, high production cost, and achieve the effects of high product yield, reduction of environmental pollution and easy operation.

Active Publication Date: 2006-11-29
山西振东泰盛制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to literature reports, the content of astragaloside IV in Radix Astragali is extremely low, about 0.04%, and the extraction and separation are very difficult (Zhang Yu, Lv Zhe, Li Xinchang, etc., the process research on the hydrolysis of total astragalosides to astragaloside IV), which is Astragaloside IV has not been developed into one of the main reasons for the clinical use of monomer preparations; there are few reports on the preparation method of astragaloside IV, and there is only one report on the preparation of astragaloside IV by RP-HPLC (Zhang Jian, Zhang Zhenhai, Yu Zengliang. RP-HPLC preparative chromatography for separation of astragaloside IV) can only be prepared in a small amount by RP-HPLC, the production cost is very high, and it is not suitable for industrial production

Method used

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  • Method for preparing purity astragaloside
  • Method for preparing purity astragaloside
  • Method for preparing purity astragaloside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 10 kg of astragalus medicinal materials, pulverize it into coarse powder, add 70% ethanol to the reflux extraction tank, add 70% ethanol to heat and reflux for 3 times, the first 120 minutes, the second and third 90 minutes each, the extracts are combined, and the ethanol is recovered under reduced pressure. Add water to dilute, put the water on the D101 macroporous adsorption resin column (10kg), and the flow rate of the sample is 2~5BV / h. The 70% ethanol of the resin bed volume is eluted, the ethanol eluent is collected, the ethanol is recovered under reduced pressure to an appropriate amount (about 1.5 kg of the extract), and 10 g of NaOH solution is added for alkaline hydrolysis. Neutralized to neutrality, followed by extraction with ethyl acetate 3 times, the aqueous phase was concentrated under reduced pressure to an appropriate volume, left to crystallize, filtered, and washed with a small amount of methanol to obtain 9 g of astragaloside IV crude product, wh...

Embodiment 2

[0027] Take 20 kg of astragalus medicinal materials, pulverize it into coarse powder, add 80% ethanol to the reflux extraction tank, add 80% ethanol for heating and reflux extraction for 3 times, each time for 90 minutes, combine the extracts, recover the ethanol under reduced pressure, add water to dilute, and add HPD100 to the water. Porous adsorption resin column (20kg), the sample flow rate is 2-5BV / h, after the liquid is applied, wash with deionized water with 4 times the volume of the resin bed, and then wash with 80% ethanol with 4 times the volume of the resin bed Take off, collect the ethanol eluent, recover ethanol under reduced pressure to an appropriate amount (extract about 3.0kg), add 20g KOH solution to carry out alkali hydrolysis, hydrolysis temperature 50 ℃, after the hydrolysis is complete, neutralize with dilute acid to neutrality, then use Extracted with ethyl acetate 4 times, concentrated the aqueous phase to an appropriate volume under reduced pressure, le...

Embodiment 3

[0029] Take 100kg of astragalus medicinal materials, pulverize it into coarse powder, add 70% ethanol to the reflux extraction tank, add 70% ethanol, heat and reflux for 3 times, the first time is 120 minutes, the second and third times are 90 minutes each, the extracts are combined, and the ethanol is recovered under reduced pressure. Diluted with water, the water was placed on a D101 macroporous adsorption resin column (100kg), and the loading flow rate was 2-5BV / h. After the drug solution was applied, wash with deionized water with 4 times the volume of the resin bed, and then 5 times the volume of the resin bed. The 80% ethanol of the resin bed volume is eluted, the ethanol eluent is collected, the ethanol is recovered under reduced pressure to an appropriate amount (about 13 kg of the extract), and 150 g of KOH solution is added to carry out alkali hydrolysis. The hydrolysis temperature is 20 ° C. And to neutrality, followed by extraction with ethyl acetate 3 times, the aq...

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Abstract

A process for preparing high-purity astragaloside from astragalus root includes such steps as extracting, concentrating, removing impurities, hydrolytic conversion, extracting in solvent and purifying.

Description

1. Technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for mass preparing pure astragaloside IV by using traditional Chinese medicine astragalus as a raw material. 2. Background technology [0002] Astragalus membranaceus (Fisch.) Bge. and Mongolian Astragalus membranaceus (Fisch.) Bge.var. mongolicus (Bge.) Hsiao are the dried roots of Astragalus membranaceus (Fisch.) Hsiao. Astragalus has the functions of invigorating qi and solidifying the surface, diuretic and toxin, expelling pus, astringing sores and promoting muscle. [0003] The main chemical constituents of Astragalus are triterpenoid saponins, flavonoids and polysaccharides. Triterpenoids mainly include astragaloside I-VIII (astragaloside I-VIII) and soybean saponin I. Among them, astragaloside IV (namely astragaloside IV) is one of the main active components in astragalus, and it is an important index component for evaluating th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 彭树林李锐丁立生廖循梁健白冰如
Owner 山西振东泰盛制药有限公司
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