Method for detecting bird flue virus H5N1 subtype based on liquid phase chip

Abstract

The present invention relates to liquid phase chip method of detecting bird flu virus H5N1 subtype. The liquid phase chip technology includes the homogeny analysis on all the H5 and N1 serum subtype nucleic acid sequence capable of being searched in the nucleic acid sequence bank by means of biological informatic means to find out conservative region, design degenerate primer and specific probe on the H5 and N1 gene fragment and couple the specific probe with fluorescent coding microsphere to make specific detecting microsphere as the liquid phase chip; and recognizing the H5 and N1 gene fragment specifically with the liquid phase chip through two rounds of PCR reaction and reading out the detection result in the chip detection instrument. The method can detect bird flu virus H5N1 subtype quickly for early diagnosis.

Description

technical field [0001] The invention relates to a method for detecting avian influenza virus H5N1 subtype based on a liquid phase chip. Background technique [0002] Influenza is one of the common acute respiratory infectious diseases. Because the influenza virus is very easy to mutate, it often causes a national or even worldwide pandemic. Especially in the recent public health emergencies such as the prevalence of avian influenza in some areas at home and abroad and the emergence of human avian influenza cases, it is urgent to make early and rapid laboratory diagnosis of the above diseases. [0003] Influenza viruses belong to the Orthomyxoviridae family and can be divided into three types: A, B, and C. According to the structure and genetic characteristics of influenza A virus surface antigens hemagglutinin antigen (HA) and neuraminidase antigen (NA), it can be divided into several subtypes. So far, it has been found that influenza A has 16 subtypes. Type (H1-H16), NA h...

AI Technical Summary

Problems solved by technology

These methods such as: reverse transcription polymerase chain reaction (reverse transcription polymerase chain reaction, RT-PCR), multiple reverse transcription polymerase chain reaction (multiplex reverse transcription-PCR, MRT-PCR), enzyme immunoassay PCR (PCR-enzymeimmunoassay, PCR -EIA), real-time PCR (real-time PCR, RT-PCR) and so on have improved the speed and sensitivity of detection, however, although more and more methods are used to detect influenza virus, there is not yet a method that can detect each influenza virus. rapid and accurate subtyping

Images