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REPLIKIN peptides and uses thereof

A peptide sequence, influenza virus technology, applied in the field of peptides with shared structural features, Replikin

Inactive Publication Date: 2006-10-18
塞缪尔·博戈奇 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, no vaccine has been effective in preventing diseases caused by these disease-causing organisms

Method used

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  • REPLIKIN peptides and uses thereof
  • REPLIKIN peptides and uses thereof
  • REPLIKIN peptides and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0435] REPLIKIN EXTRACTION, ISOLATION AND IDENTIFICATION METHODS AND USE OF REPLIKIN TO TARGET, MARK OR DESTROY REPLIKIN CONTAINING Organisms

[0436] a) Algae

[0437] The following algae were collected from the Bermuda water station and were either extracted the same day or frozen at -20°C and extracted the next day. The algae were prepared in a cold room (0-5° C.) in a Waring blender in 1 gram aliquots in a neutral buffer such as 100 cc. 0.005 M phosphate buffer pH 7 (“phosphate buffer” ) for 15 minutes, centrifuged at 3000 rpm, the supernatant was concentrated by evaporation, and then dialyzed against cold phosphate buffer to obtain a volume of approximately 15 ml. The volume of this extraction solution is recorded, an aliquot sample is taken for protein analysis, and the remaining sample is fractionated to obtain protein fractions with a pK range between 1-4.

[0438] A preferred method of fractionation is chromatography as follows:

[0439] The extraction solution was...

Embodiment 2

[0453] As an example of the diagnostic use of Replikin: Aglyco 1OB or the 16-mer Replikin can be used as an antigen to capture its corresponding antibody present in serum and quantify the amount of said antibody for diagnostic purposes, as in U.S. 6,242,578 B1 Shown in Figures 2, 3, 4 and 7.

[0454] As an example of the production of drugs linked to Replikin for labeling, nutritional or destruction purposes: the 16-mer Replikin is injected into rabbits to generate specific antibodies against the 16-mer Replikin, as shown in U.S. 6,242,578 B1 Embodiment 6 and Figure 9A and Figure 9B.

[0455] As an example of labeling Replikin with drugs: labeling specific cells containing the Replikin with an antibody against the 16-mer Replikin is shown in Figure 5 and Example 6 of U.S. 6,242,578 B1.

[0456]As an example of destroying Replikin with drugs: inhibit or destroy specific cells containing the Replikin with an antibody against the 16-mer Replikin, as shown in U.S. 6,242,578 B1 F...

Embodiment 3

[0458] The presence and concentration of Replikin in the sequence data of influenza virus hemagglutinin protein or neuraminidase protein isolates was analyzed by visual scanning of the sequence or by using the computer program described herein based on the three-point recognition system. analyze. Influenza virus isolates are obtained and amino acids of the influenza hemagglutinin and / or neuraminidase proteins are obtained by any method known in the art, for example by sequencing the hemagglutinin or neuraminidase genes and deriving protein sequences therefrom sequence. Sequences were scanned for the presence of novel Replikins, the conservation of Replikins over time, and the concentration of Replikins in each isolate. Comparing Replikin sequences and concentrations to amino acid sequences obtained from earlier (eg, about 6 months to about 3 years ago) isolates provides new variants used to predict the most likely cause of influenza in the upcoming flu season strain data and...

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Abstract

The present invention discloses influenza virus hemagglutinin protein and peptide of malaria antigen of Plasmodium falciparum (Plasmodium falciparum), antibody specific to said peptide, influenza vaccine, malaria vaccine and immunity stimulating patients to produce anti-influenza virus or malaria antibody method of response. The invention also discloses methods for formulating vaccines against influenza viruses. The invention discloses an isolated peptide of Bacillus anthracis (Bacillus anthracis) anthrax toxin lethal factor protein pX01-107, an antibody specific to the peptide and an antibody that stimulates patients to produce anti-Bacillus anthracis anthrax toxin lethal factor protein pX01-107 method of the immune response. The present invention also discloses isolated peptides of the smallpox virus surface antigen S precursor protein, antibodies specific to said peptides and methods of stimulating an immune response in a patient to produce antibodies against the smallpox virus surface antigen S precursor protein.

Description

[0001] Cross-references to other applications [0002] This application is a continuation-in-part of application numbers US 09 / 984,056 and US 09 / 984,057, filed concurrently on October 26, 2001, which claim provisional application 60 / 303,396, filed on July 9, 2001, and 2001 Priority to provisional application 60 / 278,761, filed March 27, the subject matter of which is incorporated herein by reference. field of invention [0003] The present invention relates to the identification and use of Replikins, a class of peptides with shared structural features. In particular, the invention relates to Replikins that have been identified in influenza viruses and their use in the design of influenza virus vaccines. The invention also relates to Replikins which have been identified in Bacillus anthracis and Small Pox Virus (Variola). Background of the invention [0004] Influenza is an acute respiratory disease of global importance. Despite attempts by countries and international organ...

Claims

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Application Information

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IPC IPC(8): C07K1/00
Inventor 塞缪尔·博戈奇埃伦诺尔S·博戈奇
Owner 塞缪尔·博戈奇
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