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Chitin oligose preparing process

A chitosan oligosaccharide and chitin technology, applied in the field of chitin oligosaccharide, can solve the problems of incapability of mass production, application limitations, etc., and achieve the effects of industrialized production, inhibition of degradation, and high use efficiency of enzymes

Inactive Publication Date: 2006-10-18
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosanase and chitinase are not yet mass-produced, so applications are limited
The method of preparing chitooligosaccharides by enzymatic hydrolysis with specific hydrolase has not been reported so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1. Strain cultivation and preparation of high-activity enzyme solution

[0059] Take a ring of Aeromonas caviae Q2 strain from the inclined plane and insert it into a 500ml shake flask with 100ml culture solution, shake it at 35°C for 24 hours to obtain the shake flask seed solution, and the shake flask seed medium contains peptone 1%, 5% yeast extract, 5% corn steep liquor, and 1% NaCl were inserted into cultures containing 1% glycerol, 5% yeast extract, 5% corn steep liquor, 1% NaCl and 7% bean cake powder at a 5% inoculum size. Fermented at 35°C for 24 hours in a 10L seed fermenter to obtain a seed culture solution. Add medium solution to a 100L fermenter, which contains 1% glycerin, 2.5% yeast extract, 2.5% corn steep liquor, 1% NaCl, 5% bean cake powder, sterilize at 121°C for 20min, add 10L of seed culture solution, and Cultivate for 24 hours at 35°C, with a rotation speed of 100-300r / min and an air flow of 100l / min, to obtain a fermentation broth containing chit...

Embodiment 2

[0071] 1. Strain cultivation and preparation of high-activity enzyme solution

[0072] Take a ring of Aeromonas caviae Q2 strain from the inclined plane and insert it into a 500ml shake flask with 100ml culture solution, shake it at 35°C for 24 hours to obtain the shake flask seed solution, and the shake flask seed medium contains peptone 1%, 5% yeast extract, 5% corn steep liquor, and 1% NaCl were inserted into cultures containing 1% glycerol, 5% yeast extract, 5% corn steep liquor, 1% NaCl and 7% bean cake powder at a 5% inoculum size. Fermented at 35°C for 24 hours in a 10L seed fermenter to obtain a seed culture solution. Add medium solution to a 100L fermenter, which contains 1.5% glycerin, 0.5% yeast extract, 1% corn steep liquor, 0.5% NaCl, 10% bean cake powder, sterilize at 121°C for 20min, add 10L of seed culture solution, and Cultivate for 40 hours at 25°C, 300r / min, and with an air flow of 5l / min to obtain a fermentation broth containing chitinase, centrifuge in a ...

Embodiment 3

[0086] 1. Strain cultivation and preparation of high-activity enzyme solution

[0087] Take a ring of Aeromonas caviaeQ2 strain from the inclined plane and insert it into a 500ml shake flask with 100ml culture solution, shake and culture at 37°C for 20h to obtain the shake flask seed solution, and the shake flask seed medium contains peptone 1%, 5% yeast extract, 5% corn steep liquor, and 1% NaCl were inserted into a culture containing 4% glycerol, 5% yeast extract, 3% corn steep liquor, 1% NaCl and 5% bean cake powder at a 5% inoculum size. Fermented at 37°C for 20 hours in a 10L seed fermenter to obtain seed culture solution. Add medium solution to a 100L fermenter, which contains 4% glycerin, 3% yeast extract, 2% corn steep liquor, 0.5% NaCl, 3% bean cake powder, sterilize at 121°C for 20min, add 10L of seed culture solution, and Under the conditions of 37°C, 200r / min, and ventilation rate of 150l / min, cultivate for 20 hours to obtain a fermentation broth containing chitin...

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PUM

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Abstract

The present invention relates to chitin oligose preparing process, and is especially enzyme generating fermentation process to degrade chitin colloid for preparing bioactive chitin oligose. The preparation process has simple technological path, effective inhibition of further degradation of bioactive chitin oligose, reuse of degrading enzyme and other advantages, and is suitable for industrial production. The preparation process includes: culturing Aeromonascaviae strain to obtain fermented liquid, centrifugally separating the fermented liquid to obtain enzyme supernatant; preparing chitin colloid; compounding chitin colloid buffer solution in an enzyme reactor and adding abacterial enzyme liquid for enzymolysis to obtain enzymolyzed chitin oligose liquid; four stages of membrane separation to obtain concentrate and spray drying to obtain bioactive chitin oligose product.

Description

technical field [0001] The invention relates to a chitosan oligosaccharide, in particular to a method for preparing biologically active chitosan oligosaccharides by degrading chitin colloids by fermenting and producing enzymes. Background technique [0002] Chitin is a linear polymer formed by connecting N-acetyl-2-deoxy-D glucose (GlcNAc) and a small amount of 2-amino and 2-deoxyglucose with β(1,4) glycosidic bonds. Chitin is a good substrate for chitinase, second only to cellulose in natural polymers. Because it is insoluble in common solvents, its application is limited. [0003] Because in the existing literature, the exact reference components of chitin, chitosan, chitooligosaccharides and chitooligosaccharides etc. are different, now the definitions of each noun used in this patent application are as follows: [0004] Chitin: also known as chitin, is a polymer of 2-acetylamino-2 deoxy-D-glucose linked by β-1,4 glycosidic bonds. [0005] Chitosan: chitosan obtained b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C07H3/06C12P19/04
Inventor 乔兴忠李永娴王风平肖湘
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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