Method for enzymatic synthesis of gadol glycoside through separation and coupling

A salidroside and enzymatic synthesis technology, applied in chemical recovery, fermentation, etc., can solve problems such as efficiency impact, and achieve high added value, high purity, and low cost effects

Inactive Publication Date: 2006-10-11
EAST CHINA UNIV OF SCI & TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The inventor disclosed in the document: Bioorganic & Medicinal Chemistry Letters 2004, 14: 2095-2097 and the application number is 200410016652.0, the Chinese patent application whose publication number is CN1560268A discloses a kind of water-dioxane system, using apple seed Powder catalyzed the method of glucoside reverse hydrolysis reaction to synthesize salidroside, the product concentration is up to 10g / L, but the concentration of the substrate p-hydroxyphenylethanol is as high as 345g / L, and the product is collected from the reaction solution by conventional separation methods - Hongjing Tianglycoside, therefore, the efficiency of production has been affected and further improvement is required

Method used

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Experimental program
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Effect test

Embodiment 1

[0027] Preparation of apple seed powder: take 1kg of apple seed waste from the preserved fruit factory, grind or mash it into powder, wash with ethyl acid 3 times (300ml×3), then wash with 300ml acetone once, drain and place Dry in a vacuum drying oven to obtain a total of 350g of dry apple seed powder, which is placed in a refrigerator at 4°C for later use.

Embodiment 2

[0029] Add 49mg glucose to 0.1mL potassium phosphate buffer (50mmol / L, pH6.5) and heat to dissolve. The final concentration of sugar in 1mL reaction system is 0.25mol / L; 0.383g p-hydroxyphenethyl alcohol is added to 0.9mL tert-butyl Heat and dissolve in alcohol. The final concentration of alcohol in 1mL reaction system is 2.5mol / L. After cooling, add 15mg (1.52U) of crude apple seed enzyme powder. After shaking and reacting at 50℃ for 192 hours, salidroside is analyzed and determined. The final yield was 28.2%.

Embodiment 3

[0031] Add 250mg apple seed powder, 272mg glucose, 0.5ml phosphate buffer (70mmol / L Na 2 HPO 4 -KH 2 PO 4 , PH6.0), 2.28g p-hydroxyphenethyl alcohol, 5.0ml dioxane, stopper the ground bottle stopper tightly, and seal with raw material tape, react at 45℃, 160rpm shaker for 120h.

[0032] After the reaction, 10ml of methanol was added to remove the enzyme by filtration. The filtered enzyme was washed with methanol (20ml×3). The filtrate was dried by adding anhydrous sodium sulfate, and the solvent was removed by rotary evaporation. Flash column chromatography (ethyl acetate: methanol = 13:1), 66 mg of salidroside was obtained, and the yield relative to glucose was 15.8%.

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Abstract

The invention discloses a method of reaction and disconnect-linkage enzymatic synthesis gadol glycosides. The method use waste apple seed of candied fruits factory as cheap biocatalyst, use the glucose and tyrosol as substrate, enzymatic synthesis the precious nature health products gadol glycosides in organic dissolvent-water system, the reaction miscible liquids adsorbed by aluminum oxide or other adsorbent, thought selective adsorption of eluent to separating the substrate of tyrosol and gadol glycosides, simplifying the purification process of product yield, the non-reaction alcohol can utilize circulating, the method is a new method to batch process gadol glycosides and it has large application foreground.

Description

Technical field [0001] The invention relates to a production method of salidroside, a natural product. Background technique [0002] Salidroside, or salidroside, is one of the effective ingredients of Rhodiola rosea plant. In actual research, the content of salidroside is often used to compare the quality of Rhodiola rosea. Rhodiola has a variety of pharmacological effects such as enhancing memory, improving cardiovascular and cerebrovascular system functions, enhancing immunity, anti-fatigue, anti-anoxia, anti-tumor, lowering blood sugar, disturbing viruses, anti-microwave radiation, and protecting organs from free radical damage. The application prospect is broad. In terms of application scope, it has developed from "adapted" drugs to rhodiola oral liquid, rhodiola food, rhodiola beverage and so on. Rhodiola preparation can improve the physical strength and intelligence of volunteer subjects, increase the work ability index, and reduce the work error rate especially in the case...

Claims

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Application Information

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IPC IPC(8): C12P19/44
CPCY02P20/582
Inventor 许建和郁惠蕾林国强
Owner EAST CHINA UNIV OF SCI & TECH
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