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Method for preparing immobilized lipase attapulgite clay

A technology for immobilizing lipase and attapulgite, applied in directions such as hydrolase immobilized on or in an inorganic carrier, can solve problems such as no reports on large-scale industrialization, and improve the utilization rate and application scope of the enzyme , good guiding role, the effect of expanding the scope of application

Active Publication Date: 2006-10-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various silicate clays are used abroad to immobilize enzymes. Although there are related studies on enzyme immobilization in China, there are few studies on using silicates as enzyme carriers, and there are no reports of large-scale industrialization.

Method used

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  • Method for preparing immobilized lipase attapulgite clay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The attapulgite used is the raw ore of attapulgite collected from Xuyi, and its composition and content composition are shown in Table 1:

[0039] Element

MgO

al 2 o 3

SiO 2

SO 2

K 2 o

CaO

TiO 2

MnO

Fe 2 o 3

Content (%)

8.2

11.9

63.8

1.0

1.9

1.6

1.4

0.5

9.7

[0040] The lipase activity used is 5000u / g.

[0041] (1) Acid treatment of attapulgite: add 300 ml of 2mol / l hydrochloric acid to 30 grams of attapulgite, stir at 50°C for 3 hours, centrifuge, then wash with deionized water until detected with 0.1mol / l silver nitrate solution After being free of chloride ions, it is dehydrated by centrifugation, dried and pulverized.

[0042] (2) Calcium chloride treatment: Weigh 20g of the above-mentioned acid-treated attapulgite, add 1mol / l of CaCl 2 Solution 50ml, stirred at 55°C for 1 hour, centrifuged at a slow speed (to prevent attapulgite from accumulat...

Embodiment 2

[0048] Acid treatment of attapulgite: add 300 ml of 1 mol / l hydrochloric acid to 30 grams of attapulgite, stir at 50°C for 4 hours, centrifuge, then wash with deionized water until no chloride ion is detected with 0.1 mol / l silver nitrate solution Finally, centrifugal dehydration, drying, crushing.

[0049] The used attapulgite raw material and other techniques are the same as in Example 1. The average unit adsorption capacity and the average enzyme activity of lipase on the attapulgite in the resulting product are close to the results of Example 1.

Embodiment 3

[0051] Acid treatment of attapulgite: add 300 ml of 6mol / l hydrochloric acid to 30 grams of attapulgite, stir at 50°C for 0.5 hours, centrifuge, then wash with deionized water until no chloride ion is detected with 0.1mol / l silver nitrate solution Finally, centrifugal dehydration, drying, crushing.

[0052] The used attapulgite raw material and other techniques are the same as in Example 1. The average unit adsorption capacity and the average enzyme activity of lipase on the attapulgite in the resulting product are close to the results of Example 1.

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Abstract

This invention provides a process for preparing immobilized lipase attapulgite, belonging to the technological field of attapulgite utilization and lipase immobilization. The invention utilizes attapulgite and lipase as raw material and comprises the following steps: attapulgite treatment, lipase immobilization by adsorption, using the absorption of lipase as indication, taking regard of the enzyme activity of immobilized lipase, achieving the ideal process of attapulgite treatement and lipase immobilizatin. The invention has beneficial effects of realizeing improved activity, multiple utilization, high utilization ratio and broader range of application of lipase, laying the foundation for lipase immobilization technology. Furthermoer, the invention enlarges the application range of attapulgite, increases the added value of attapulgite and has good guiding effects to use attapulgite as enzyme vectors.

Description

technical field [0001] The invention discloses a method for preparing immobilized lipase attapulgite, which belongs to the technical field of attapulgite utilization and lipase immobilization. Background technique [0002] Attapulgite is a kind of hydrous-rich magnesium aluminum silicate clay mineral, which has a unique layer chain crystal structure and very fine (about 0.01μm×1μm) rod-like and fibrous crystal forms, and is a natural adsorbent. It can be used as adsorbent, catalyst, carrier, etc. my country's attapulgite resources are very rich, in my country's Anhui Mingguang, Jiangsu Xuyi, Liuhe and other places are rich in reserves, only Xuyi County's existing attapulgite reserves are more than 67 million tons, accounting for about 70% of my country's total attapulgite. The theoretical chemical formula of attapulgite crystals is: Mg 5 Si 8 o 20 (OH) 2 (OH 2 ) 4 4H 2 O. As a biocatalyst, enzymes have the advantages of mild reaction conditions, biodegradability, lo...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N9/16
Inventor 王兴国黄健花刘元法金青哲单良
Owner JIANGNAN UNIV
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