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Process for extraction preparation of active zinc metallothionein in sea cucumber and abalone

A zinc-sulfur protein and production process technology, which is applied in the field of extraction and production process of sea cucumber and abalone active zinc-sulfur protein, can solve the problems of extracting active zinc-sulfur protein that have not been seen yet, and achieve simple production process, extraction rate and purification high rate effect

Inactive Publication Date: 2006-10-11
YANTAI HAICHENG BIONT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the process of extracting active zinc-sulfur protein from sea cucumber and abalone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of extraction production process of sea cucumber and abalone active zinc-sulfur protein of the present embodiment, its procedure comprises feed induction, raw material slaughter cleaning, raw material crushing, filtration, homogenate, crystallization extraction process,

[0022] In the slaughtering and cleaning process, fresh sea cucumbers and abalones containing active zinc-sulfur protein are slaughtered and cleaned, and washed repeatedly with distilled water to remove all impurities;

[0023] In the raw material crushing process, put the cleaned live sea cucumbers and abalones into a crusher for crushing, pass through a 60-200 mesh sieve, put them in a water container with a temperature of 10°C, add NaOH or NaCO3, and the pH value of the water reaches 7, Stand for 10 hours;

[0024] In the filtration process, the homogenate is filtered by a centrifuge, the liquid is retained, and the residue is removed;

[0025] In the homogenization process, the above liquid ...

Embodiment 2

[0028] The extraction production process of a kind of sea cucumber and abalone active zinc-sulfur protein of the present embodiment, differs from embodiment 1 in that

[0029] In the raw material crushing process, put the cleaned live sea cucumbers and abalones into a crusher for crushing, pass through a 60-200 mesh sieve, put them in a water container with a temperature of 30°C, add NaOH or NaCO3, and the pH value of the water reaches 8. Stand for 15 hours;

[0030] In the filtration process, the homogenate is placed in a filter cloth for natural filtration, and the filtrate is taken;

[0031] In the homogenization process, the above liquid is filtered, and the filtered liquid and liquid ethanol are mixed and homogenized according to the liquid volume ratio of 1:4, and the content of ethanol is 85%;

[0032] In the crystallization extraction process, the temperature in the distillation pot in the vacuum distillation process is 55°C; the temperature in the freeze separation p...

Embodiment 3

[0034] The extraction production process of a kind of sea cucumber and abalone active zinc-sulfur protein of the present embodiment, differs from embodiment 1 in that

[0035] In the raw material crushing process, put the cleaned live sea cucumbers and abalones into a crusher for crushing, pass through a 60-200 mesh sieve, put them in a water container at a temperature of 20°C, add NaOH or NaCO3, the pH value of the water reaches 7.5, Stand for 20 hours;

[0036] In the homogenization process, the above liquid is filtered, and the filtered liquid and liquid ethanol are mixed and homogenized according to the liquid volume ratio of 1:2.8, and the ethanol content is 73%;

[0037] In the crystallization extraction process, the temperature in the distillation pot in the vacuum distillation process is 45°C; the temperature in the freeze separation process is -85°C, and it is kept for 36 hours, crystallized 3 times, and the sea cucumber and abalone zinc-sulfur protein crystals are ex...

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PUM

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Abstract

This invention belonging to metallothionin extraction field is about the extraction procedure of active zinc-sulfur protein from sea cucumber and abalone. The whole procedure involves feedstuff inducing, cleaning the raw material, squashing, filtering, homogenate, crystallization and extraction. With the procedure we reported here, most of sea cucumber and abalone'effective efficiency is remained, the purity of our product is up to 90%, and the procedure is easy to manipulate. We could extract 300g zinc-sulfur protein from 500g dried sea-cucumber or extract 300g active zinc-sulfur protein from 500g alive sea-cucumber.

Description

1. Technical field: [0001] The invention relates to a production process for extracting active zinc-sulfur protein from sea cucumber and abalone, and belongs to the technical field of metallothionein extraction methods. 2. Background technology: [0002] In 1957, when Margoshes and Vallee were studying the biological effects of metals, a new protein was isolated from animal organs. It contains rich sulfhydryl groups and can chelate a large amount of metal ions. This substance is called metallothionein (Metallothionein in English, Abbreviated as MT). Human body, animals, plants and microorganisms all contain MT, and their physical and chemical properties are basically the same. The molecular weight is 6500-7000 Daltons, the diameter is 30-50A, it is divided into two structural domains, each molecule contains 7-12 metal atoms, and has special light absorption. It contains 61 amino acids, 21 of which are cysteine, so that each MT molecule can bind up to seven metal atoms. MT...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K14/435
Inventor 黄世宽
Owner YANTAI HAICHENG BIONT
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