Method of united fixing desert barren sand by utilizing thallose
A combination of fixation and desert technology, applied in gardening methods, botanical equipment and methods, soil protection, etc., can solve problems such as slow growth, and achieve the effect of simple equipment, omitting operation steps, and convenient operation
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Embodiment 1
[0026] Select 10g of soil samples from the Junggar Basin, Xinjiang, my country, 2cm below the surface crust, mix them with sterile water, put them into a 90ml triangular flask with glass beads, vibrate for 20min, put the triangular flask in boiling water and heat it for 10min Made 10 -2 、10 -3 、10 -4 、10 -5 4 different dilutions of the soil suspension, 0.2ml of each dilution of the soil suspension was inoculated on the plate containing the bacterial beef extract peptone medium, and the medium plate was inverted and cultured in a 37°C incubator for 2 sky. Use the spore staining method to identify the cultured strains, dry and fix the cultivated thallus smear, and observe it under the oil microscope after dyeing and drying with the dye solution. The heat-resistant soil bacillus that is red; the classification of this soil bacillus is named: Bacillus sp, which was preserved in the "General Microbiology Center of China Microbiological Culture Collection Management Committee" on...
Embodiment 2
[0035] Select 10g of soil samples from the Junggar Basin in Xinjiang, my country, 6cm below the surface crust, mix them with sterile water, put them into a 90ml triangular flask with glass beads, vibrate for 20min, put the triangular flask in boiling water and heat it for 10min Made 10 -2 、10 -3 、10 -4 、10 -5 4 different dilutions of the soil suspension, 0.2ml of each dilution of the soil suspension was drawn and inoculated on the plate containing the bacterial beef extract peptone medium, and the medium plate was inverted and cultivated in a 37°C incubator for 3 sky. Use the spore staining method to identify the cultured strains, dry and fix the cultivated thallus smear, and observe it under the oil microscope after dyeing and drying with the dye solution. A red heat-tolerant Agrobacterium.
[0036] The above-mentioned Agrobacillus isolated from the soil crust was fermented on a large scale using optimized cheap liquid medium I-2. The composition of this liquid medium I-2...
Embodiment 3
[0041] Select 10g of soil samples from 10cm below the surface crust of the Junggar Basin in Xinjiang, my country, mix them with sterile water, put them into a 90ml triangular flask with glass beads, vibrate for 20min, put the triangular flask in boiling water and heat it for 10min Made 10 -2 、10 -3 、10 -4 、10 -5 4 different dilutions of the soil suspension, 0.2ml of each dilution of the soil suspension was drawn and inoculated on the plate containing the bacterial beef extract peptone medium, and the medium plate was inverted and cultivated in a 37°C incubator for 3 sky. Use the spore staining method to identify the cultured strains, dry and fix the cultivated thallus smear, and observe it under the oil microscope after dyeing and drying with the dye solution. A red heat-tolerant Agrobacterium.
[0042] The above-mentioned soil bacillus isolated from the soil crust is fermented on a large scale using optimized cheap liquid medium I-3, the composition of this liquid medium ...
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