Fluid-drop-flowing injection device and quantitative analysing method

Abstract

A liquid drop flow injection unit and quantitative analysis method includes constant flow pump, injector pump, peristaltic pump, negative high pressure controller high pressure controller, chemoluminescence instrument, and computer. Quantitative analysis method includes preparation reagent, preparing sample solution, standard curve and detecting sample. Said invented liquid drop flow injection unit and quantitative analysis method can be used in inorganic ions, organic molecule, medicine etc flow injection analysis.

Description

technical field [0001] The invention belongs to the technical field of testing or analyzing materials by means of determining their chemical or physical properties, and in particular relates to devices in which materials are subjected to chemical reactions, and the progress of the testing reactions or the resulting chemiluminescence. Background technique [0002] Since Ruzicka and Hansen first proposed Flow Injection Analysis (FIA) technology in 1975, flow injection technology combined with detection methods such as ultraviolet, fluorescence, chromatography, atomic spectroscopy, and mass spectrometry has been widely used in the field of analytical chemistry. application. The characteristics of fast, simple and low consumption of flow injection technology are also the goals pursued by all analytical methods. Among the analysis methods combined with flow injection technology, the luminescence analysis method shows the advantages of fast analysis speed, simple equipment, less ...

AI Technical Summary

Problems solved by technology

[0003] In the traditional flow injection analysis method, since the carrier is usually used to carry the sample zone and mix it with the reaction reagent, in the process of carrying the sample zone and mixing with the reaction reagent, the sample will be diluted due to diffusion, which makes the analysis method Sensitivity is affected

When using a sampling loop to control the injection volume, a large amount of sample solution is required to wash the sampling loop pipe before each injection, which increases the consumption of reagents or samples

In the traditional flow injection analysis process, the reagent is usually in a continuous flow state, and the reagent consumption in the interval between two injections is also very large

When the detection object of the flow injection analysis is the reaction product of the sample and the reagent, not only the sample and the reagent solution are required to be fully mixed, but also the sample and the reagent are mixed to achieve an optimal mixing ratio; in the traditional flow injection, due to the fluid The laminar flow state is in a dominant position in the pipeline. In order to realize the full mixing of the sample and the reagent solution, it is necessary to obtain turbulent flow by weaving the reactor and other means; in the sequential injection analysis, the sample and the reagent solution are realized by the reciprocating motion of the solution. However, these methods will lead to excessive dilution of the reaction product, making it difficult to ensure the optimal mixing ratio of sample-reagent

Similarly, it takes a certain amount of time to fully mix the above samples and reagents, and the analysis frequency of the analytical method is also greatly affected.

[0004] In the disclosed droplet analysis instruments, only the volume measurement of a single droplet and the analysis of the fluorescent components in a single droplet are introduced, and the coalescence of two or more droplets and the coalescence of droplets are not involved. reaction process

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