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Use of gingko endogenous fungus metabolite in preventing and treating plant fungus disease

A plant fungal disease, endophytic fungus technology, applied in the application, plant growth regulator, botanical equipment and methods, etc., to achieve the effect of improving the success rate and efficiency

Inactive Publication Date: 2006-07-19
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the isolation and screening of endophytic fungi from Ginkgo biloba and the application of their metabolites in the prevention and treatment of plant fungal diseases have not been reported at home and abroad.

Method used

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  • Use of gingko endogenous fungus metabolite in preventing and treating plant fungus disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Ginkgo stem tissues were collected from the Ginkgo Corridor in Changxing, Zhejiang, and the surface was disinfected according to the following procedures: rinse with tap water, rinse with 75% alcohol for 5 minutes, rinse with sterile water for 5 times, soak in mercuric chloride for 4 minutes, and rinse with sterile water for 3 times; The sterilized stem tissue is directly planted on a PDA medium plate, and cultured in a 28°C incubator, from which the stem tissue without any bacterial growth on the surface is selected;

[0020] The aseptically tested stem (phloem) tissue was cut into small pieces of about 0.5 cm × 0.5 cm under ultra-clean workbench conditions, and then the small pieces were planted on the PDA medium plate and cultivated in a 28°C incubator; When mycelium grows obviously around the sample, the tip mycelia picking method is used to pick colonies with different shapes, and after purification, they are transferred to PDA slant medium for later use;

[0021] ...

Embodiment 2

[0027] Take ginkgo leaves and disinfect the surface according to the following procedures: rinse with tap water, rinse with 75% alcohol for 5 minutes, rinse with sterile water for 5 times, soak in mercuric chloride for 4 minutes, rinse with sterile water for 3 times; the leaves after surface disinfection are directly planted on PDA Put it on the medium plate and culture it in a 27°C incubator, and select the tissue without any bacteria growing on the surface;

[0028] Cut the leaves tested for sterility into small pieces of about 0.5cm×0.5cm under ultra-clean workbench conditions, then plant the small pieces on PDA plates, and place them in an incubator at 27°C for cultivation; when mycelium grows obviously around the sample , using the tip mycelia picking method to pick colonies with different shapes, and transfer them to PDA slant medium after purification, and set aside;

[0029] Each bacterial strain of the above-mentioned isolated ginkgo endophytic fungus is carried out l...

Embodiment 3

[0033] Get the ginkgo pulp (exotesta), and disinfect the surface according to the following procedures: rinse with tap water, rinse with 75% alcohol for 5 minutes, rinse with sterile water for 5 times, then soak with mercuric chloride for 4 minutes, rinse with sterile water for 3 times; The pulp tissue is directly planted on a PDA medium plate, cultured in a 26°C incubator, and the fruit tissue without any bacterial growth on the surface is selected;

[0034] Cut the pulp that has been tested for sterility into small pieces of about 0.5cm×0.5cm under ultra-clean workbench conditions, and then plant the small pieces on a PDA plate and place them in a 26°C incubator for cultivation; when hyphae grow obviously around the sample , using the tip mycelia picking method, pick colonies with different shapes, after purification, transfer to PDA slant medium for future use.

[0035] Each bacterial strain of the above-mentioned isolated ginkgo endophytic fungus is carried out liquid ferm...

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Abstract

The invention discloses an application of ginko endogenetic fungi metabolite in preventing plant mycosis, belonging to microbiological pesticide technology, which comprises the following steps: preparing ginko tissue, surface sterilizing, separating and culturing the endogenetic fungi, sifting effective strain, fermenting and preparing bactericide. The invention provides a new way for developing agricultural bactericide.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to the application of ginkgo biloba endophytic fungus metabolites in the prevention and treatment of plant fungal diseases. Background technique [0002] Ginkgo biloba L., also known as ginkgo, is a rare and precious tree species in the world unique to my country. It is a relic plant left over from glacier movement and is called a living fossil. It belongs to the ginkgo family and is the only existing species of the genus Ginkgo. Now it is a widely cultivated tree species with high research and development value. Each tissue of Ginkgo biloba contains antibacterial substances. According to reports, the extracts of Ginkgo biloba roots, stems, leaves, pulp (exotesta) and nuts have inhibitory effect on the tested plant pathogenic bacteria. However, the amount of plant resources is one of the main factors limiting the development scale of botanica...

Claims

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Application Information

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IPC IPC(8): A01N63/00
Inventor 申屠旭萍陈列忠俞晓平
Owner CHINA JILIANG UNIV
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