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Prolan enzyme bacterial and preparation process

A technology of pullulanase and producing bacteria, applied in the field of pullulanase producing bacteria and preparation, and can solve problems such as enzyme inactivation

Inactive Publication Date: 2006-06-28
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the acid-resistant and heat-resistant pullulanase strains reported at home and abroad are only the above three strains, but they have a common deficiency. When the temperature exceeds 65 ° C, or the pH value is lower than 3.6, the enzyme quickly Inactivate

Method used

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  • Prolan enzyme bacterial and preparation process
  • Prolan enzyme bacterial and preparation process
  • Prolan enzyme bacterial and preparation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1. Isolation of strains:

[0035] Water samples from Tengchong Hot Spring in Yunnan Province and soil samples around the hot spring were collected. During the experiment, 5g of the soil sample was weighed into 45ml of normal saline, and glass beads were added to fully shake and then allowed to stand for 1 hour. Take 100 μl of the supernatant of the soil sample in a test tube, do gradient dilution, take an appropriate dilution to smear on a plate, and incubate in an electric constant temperature incubator at 50°C for 48hrs. Drop an appropriate amount of iodine solution with a concentration of 1% on the separation plate, pick the strains with blue transparent circles, separate and purify by streaking, culture at 50°C for 24 hours, and then transfer the strains with blue transparent circles into the identification medium. After culturing in the incubator for 48 hours, select the strains that can produce white decomposition circles on the red pullulan plate and continue to ...

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PUM

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Abstract

The invention relates to pullulanase producing strain and manufacturing method. It is named alieycloeacillus. And its preservation register number is CGMCC No.1504. The formed strain D-1 is one new species of alieycloeacillus. Its pullulanase pH is 3.5-4.5. If pH is higher then 5.0 or lower than 3.0, enzyme is inactive; if it is 4.0, residue enzyme energy is 88%; the optimum temperature is 60 centigrade degree; enzyme reaction is not need metal ion while temperature is 55-65 centigrade degree. Thus the enzyme can be used in starch processing saccharification stage. Compared with acidity pullulanase, the enzyme has better heat resistance and pH stability.

Description

technical field [0001] The invention relates to a strain producing pullulanase and a preparation method thereof. Background technique [0002] Pullulanase (Pullululanase, EC.3.2.1.41) is a debranching enzyme that can specifically cut the α-1,6 glycosidic bond in the branch point of amylopectin, thereby cutting off the entire side branch to form amylose . It has a very important use in the starch processing industry. When pullulanase and glucoamylase are used together, while reducing the amount of glucoamylase by more than half, the DE value can be as high as 98%, thereby greatly increasing the yield of glucose; when used in the alcohol industry, it can greatly increase the conversion rate of starch and reduce Cost of production. When it is mixed with β-amylase, almost all starch can be converted into maltose, so that more than 80% of ultra-high maltose syrup can be produced, and under the action of α-glucosidase, ultra-high maltose syrup can be converted into functional ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/44C12N15/31C12N15/56C12R1/07
Inventor 黄遵锡陈金全杨云娟唐湘华
Owner YUNNAN NORMAL UNIV
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